1/22/20142 ENZYMES Globular Proteins Active Site One Substrate only Induced Fit: Changes occur when meet with substrate
1/22/20143 ENZYME CATALYSIS Enzymes Catalysis - Activation Energy - Faster Reaction without change of form. Factors affecting change: pH, temperature
1/22/20144 BEFORE THE LAB Find out rate - enzyme catalase- substrate product Hydrogen peroxide = substrate amount of hydrogen peroxide remain after reaction titration with KMnO 4 Titration - add KMnO 4 - color change until target color
1/22/20145 LAB 1. Add 10ml of HOto each of 7 beakers. 2. Add 1ml of catalase to the first beaker at 0 second. 3. Observe the reaction until the time labeled on the beaker. 4. Stop the reaction by adding 10ml of H 2 SO 4 5. Repeat the process for other remaining beakers.
1/22/20146 TITRATION 1. Remove a 5ml sample for titration and move the sample to a clean flask. 2. Record initial burette reading. 3. Add KMnO 4 (purple) until faint brown color persists (it is the endpoint of process.) 4. Record final burette reading. 5. Calculate the ml of KMnO 4 used to reach the endpoint. 6. Repeat the process for other remaining beakers.
1/22/20147 * KMnO 4 to the flask - mix - lose color - all peroxide reacted with KMnO 4 additional KMnO 4 light brown or pinkish * KMnO 4 - peroxide We calculate the rate of a reaction by measuring or observing the disappearance of substrate or the appearance of product. This lab figured out the rate at which the enzyme catalase converts substrate to product by observing the disappearance of substrate.
1/22/20148 Bibliography 1.LabBench http://www.phschool.com/science/biology_place/labb ench/lab2/active.html 2. Drug Strategies to Target HIV http://www.chemistry.wustl.edu/~edudev/LabTu torials/HIV/DrugStrategies.html http://www.chemistry.wustl.edu/~edudev/LabTu torials/HIV/DrugStrategies.html