3 Remember what it means to be a plant… Need to produce all organic molecules necessary for growthcarbohydrates, lipids, proteins, nucleic acidsNeed to store chemical energy (ATP) produced from light reactionsin a more stable formthat can be moved around plantsaved for a rainy day+ water + energy glucose + oxygencarbondioxide6CO26H2OC6H12O66O2lightenergy+
4 build stuff !! photosynthesis Light reactionsConvert solar energy to chemical energyATPNADPHWhat can we do now?ATP energy reducing power build stuff !!photosynthesis
5 CO2 C6H12O6 How is that helpful? Want to make C6H12O6 synthesis How? From what? What raw materials are available?CO2CO2 contains little energy because it is fully oxidizedReduce CO2 in a series of steps to synthesize a stable energy storage moleculeNADPHreduces CO2carbon fixationNADPNADPC6H12O6
6 From CO2 C6H12O6 CO2 has very little chemical energy fully oxidizedC6H12O6 contains a lot of chemical energyhighly reducedSynthesis = endergonic processput in a lot of energyReduction of CO2 C6H12O6 proceeds in many small uphill stepseach catalyzed by a specific enzymeusing energy stored in ATP & NADPH
7 From Light reactions to Calvin cycle chloroplast stromaNeed products of light reactions to drive synthesis reactionsATPNADPHstromaATPthylakoid
8 Calvin cycle 1C 5C 6C 5C 3C 3C 3C CO2 RuBP RuBisCo 3 ATP 3 ADP used 1. Carbon fixationC3. Regeneration of RuBPC5CCRuBPRuBisCoCribulose bisphosphate6Cstarch, sucrose, cellulose & more3 ADP3 ATPCribulose bisphosphate carboxylase5CCusedto make glucoseCglyceraldehyde-3-PC1. A five-carbon sugar molecule called ribulose bisphosphate, or RuBP, is the acceptor that binds CO2 dissolved in the stroma. This process, called CO2 fixation, is catalyzed by the enzyme RuBP carboxylase, forming an unstable six-carbon molecule. This molecule quickly breaks down to give two molecules of the three-carbon 3-phosphoglycerate (3PG), also called phosphoglyceric acid (PGA).2. The two 3PG molecules are converted into glyceraldehyde 3-phosphate (G3P, a.k.a. phosphoglyceraldehyde, PGAL) molecules, a three-carbon sugar phosphate, by adding a high-energy phosphate group from ATP, then breaking the phosphate bond and adding hydrogen from NADH + H+.3. Three turns of the cycle, using three molecules of CO2, produces six molecules of G3P. However, only one of the six molecules exits the cycle as an output, while the remaining five enter a complex process that regenerates more RuBP to continue the cycle. Two molecules of G3P, produced by a total of six turns of the cycle, combine to form one molecule of glucose.3CPGAG3PCphosphoglycerateCCC3CC=CCC6 ADP6 ATP2. ReductionC|HC–C6 NADP6 NADPH3CC
9 glyceraldehyde 3-phosphate glucoseC-C-C-C-C-CRemember G3P?ATP2ADP2glycolysisfructose-1,6bPP-C-C-C-C-C-C-Pglyceraldehyde 3-phosphateDHAPP-C-C-CG3PC-C-C-PNAD+2Gluconeogenesis == making new glucose2ADP4ATP4pyruvateC-C-CPhotosynthesis
10 To G3P and Beyond! Glyceraldehyde-3-P G3P is an important intermediate end product of Calvin cycleenergy rich 3 carbon sugar“C3 photosynthesis”G3P is an important intermediateG3P glucose carbohydrates lipids phospholipids, fats, waxes amino acids proteins nucleic acids DNA, RNA
11 It’s not easy being green! RuBisCoEnzyme which fixes carbon from airribulose bisphosphate carboxylasethe most important enzyme in the world!it makes life out of air!definitely the most abundant enzymeIt’s not easy being green!I’m green with envy!
12 Accounting The accounting is complicated 3 turns of Calvin cycle = 1 G3P3 CO2 1 G3P (3C)6 turns of Calvin cycle = 1 C6H12O6 (6C)6 CO2 1 C6H12O6 (6C)18 ATP + 12 NADPH 1 C6H12O6any ATP left over from light reactions will be used elsewhere by the cell
14 Light Reactions produces ATP produces NADPH H2OATPO2lightenergy+NADPHH2Osunlightproduces ATPproduces NADPHreleases O2 as a waste productEnergy BuildingReactionsNADPHATPO2
15 Calvin Cycle builds sugars uses ATP & NADPH recycles ADP & NADP CO2 C6H12O6+NADPATPNADPHADPCO2builds sugarsuses ATP & NADPHrecycles ADP & NADPback to make more ATP & NADPHADPNADPSugar BuildingReactionsNADPHATPsugars
16 Putting it all together CO2H2OC6H12O6O2lightenergy+H2OCO2Plants make both:energyATP & NADPHsugarssunlightADPNADPEnergy BuildingReactionsSugar BuildingReactionsNADPHATPO2sugars
17 Energy cycle ATP Photosynthesis Cellular Respiration sun CO2 O2 H2O even though this equation is a bit of a lie… it makes abetter storysunEnergy cyclePhotosynthesisCO2H2OC6H12O6O2lightenergy+H2OplantsCO2glucoseO2animals, plantsCO2H2OC6H12O6O2ATPenergy+Cellular RespirationATPThe Great Circle of Life,Mufasa!
18 Summary of photosynthesis 6CO26H2OC6H12O66O2lightenergy+Where did the CO2 come from?Where did the CO2 go?Where did the H2O come from?Where did the H2O go?Where did the energy come from?What’s the energy used for?What will the C6H12O6 be used for?Where did the O2 come from?Where will the O2 go?What else is involved…not listed in this equation?Where did the CO2 come from? airWhere did the CO2 go? carbohydrate (G3P)Where did the H2O come from? soil (xylem)Where did the H2O go? split to donate e- & H+Where did the energy come from? sunWhat’s the energy used for? to make ATP in light reactionsWhat will the C6H12O6 be used for? the work of plant lifeWhere did the O2 come from? splitting of H2OWhere will the O2 go? air or respirationWhat else is involved…not listed in this equation?ATP, ADP, NADP, NADPH
19 Supporting a biosphere On global scale, photosynthesis is the most important process for the continuation of life on Eartheach year photosynthesis…captures 121 billion tons of CO2synthesizes 160 billion tons of carbohydrateheterotrophs are dependent on plants as food source for fuel & raw materials
20 The poetic perspective… All the solid material of every plant was built by sunlight out of thin airAll the solid material of every animal was built from plant materialsunairThen all the plants, cats, dogs, elephants & people … are really particles of air woven together by strands of sunlight!
21 If plants can do it… You can learn it! Ask Questions!!