Presentation on theme: "AP Lab 7: Genetics of Drosophila (fruit flies) Carolina."— Presentation transcript:
AP Lab 7: Genetics of Drosophila (fruit flies) Carolina
Purpose Use fruit flies to do genetic crosses. Learn to determine the sex of fruit flies and recognize contrasting phenotypes. Collect data from F 1 and F 2 generations and analyze the results of a monohybrid, dihybrid, or sex-linked cross.
Activity A In part A you will learn to: observe wild-type flies recognize male and female flies compare the mutant phenotypes with the wild type. http://www.phschool.com/science/biology_ place/labbench/index.html http://www.phschool.com/science/biology_ place/labbench/index.html
Procedure Anesthetizing flies will be demonstrated. Flies will be placed in petri dishes to be observed. Draw and document important differences between phenotypes of: Males and females Wild types and mutants
Procedure After you have observed the flies, return them to the correctly labeled morgue. Make sure that if flies begin moving they are returned to the morgue quickly. The anesthetizing agent lasts approximately 50 min.
Activity B Setting up vials: Obtain one vial and add 1 level measure cup of Formula 4-24 to the vial. Add 1 level measure cup of tap water to the vial. After it solidifies, sprinkle 4-7 grains of yeast to the culture
Procedure Setting up F 1 cross: Record the code for the cross that you were given. Collect 5-6 male/female pairs. (10-12 flies total) Using Table 2: F 1 Data, record sex and phenotypes for the flies you were given. Place these flies in your vial and label with information from the lab. Return remaining flies to the morgue.