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Intro to Labs 1-4 Labs n stuff

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Slide 2 of 20 Lab #1 – Diffusion & Osmosis 4 Parts 1. Diffusion – Qualitative 2. Osmosis – Quantitative & Graph 3. Molar Concentration – Quantitative, Graph, and Inference 4. Water Potential – Calculation 4b. Plasmolysis – if you have not scared or scarred me.

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Slide 3 of 20 Lab #1 – Diffusion & Osmosis Should get good results Simple Understand what you are doing, dont just follow the steps!! Think of this lab as intro to AP Labs

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Slide 4 of 20 Lab #1 – Part 1 You will put two substances in a bag made of dialysis tubing. You will determine whether they exit/enter the dialysis tubing bag. To do this, you will use either indicator strips or IKI (potassium Iodide or Lugols solution) Data (color) will be recorded in a table

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Slide 5 of 20 Lab #1 – Part 2 Fill dialysis bags with different concentrations of sucrose solution, place bags in beaker with water. 0.0 M 0.2 M 0.4 M 0.6 M 0.8 M 1.0 M Weigh each (dialysis bag + sucrose solution) BEFORE and AFTER to calculate Percent Change in Mass Make line graph

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Slide 6 of 20 Lab#1 – Part 2 Why do we use sucrose solution? What would change if we used a salt solution? Which bags will increase in mass? Which bags will decrease in mass? What is responsible for the increase/decrease in mass?

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Slide 7 of 20 Lab #1 – Part 3 Pieces of potato (or other vegetable) placed in various sucrose solutions What sucrose concentrations we will use? Measure mass of potato before being placed in solutions to calculate Percent Change in Mass Must be left Overnight.

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Slide 8 of 20 Lab#1 – Part 3 Why do we use sucrose solution? Which potato samples will increase in mass? Which potato samples will decrease in mass? What is responsible for the increase/decrease in mass?

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Slide 9 of 20 Lab #1 – Part 4 Calculate Water Potential Water potential = Solute potential + Pressure potential Ψ = Ψs + Ψp Ψs = -iCRT i = 1 C = Molar Concentration R = (liter*bar)/(mole*K) T = temperature in Kelvin

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Slide 10 of 20 What to do next? Organize yourselves into groups of 3-4 Each group is responsible for getting the following: 12 clear or translucent plastic cups (10oz or more) Sharpie Permanent Marker Label Cups Name of group + Period 2 sets of (0.0M, 0.2M, 0.4M, 0.6M 0.8M 1.0M)

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Slide 11 of 20 When? Friday 12/2 Lab #2 Activity (Toothpickase) Lab #3 Virtual Lab Monday 12/5 Start Lab #1 & Pre-Lab Quiz (12/5) Tuesday 12/6 Finish Lab #1 Start Lab #4 Pre-Lab Quiz – Lab #4 TEST: Labs 1-4 on Monday (12/12)

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Slide 12 of 20 Lab #2 – Enzyme Kinetics We will do a modified version of Lab #2 Toothpickase Self-Contained Lab Exercise Separate yourself into groups of 3-4 Need: Approximately 600 flat toothpicks Approximately 100 round or reinforced toothpicks Only need to be harder to break than the flat toothpicks

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Slide 13 of 20 Lab #3 – Mitosis & Crossing Over Virtual Lab Exercise Data Group Assignment (1-8) by teacher. First, identify cells in various stages of Mitosis (including interphase) Second, calculate map units and draw conclusions

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Slide 14 of 20 Lab #4 - Photosynthesis 2 nd Actual Lab 2 Parts Chromatography (Semi-FAIL) Part that fails miserably Fingers Crossed? IF worse comes to worse, you will just analyze given data

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Slide 15 of 20 Lab #4 – Part 1

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Slide 16 of 20 Lab #4 – Calculate Rf values

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Slide 17 of 20 Chromatography ench/lab4/quiz1.html ench/lab4/quiz1.html Use Online Animations: ench/lab4/concepts2.html ench/lab4/concepts2.html

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Slide 18 of 20 Part that goes well

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Slide 19 of 20 Part that does not go well

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Slide 20 of 20 Results Analysis

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