2Lab #1 – Diffusion & Osmosis 4 Parts1. Diffusion – Qualitative2. Osmosis – Quantitative & Graph3. Molar Concentration – Quantitative, Graph, and Inference4. Water Potential – Calculation4b. Plasmolysis – if you have not scared or scarred me.
3Lab #1 – Diffusion & Osmosis Should get good resultsSimpleUnderstand what you are doing, don’t just follow the steps!!Think of this lab as intro to AP Labs
4Lab #1 – Part 1You will put two substances in a bag made of dialysis tubing.You will determine whether they exit/enter the dialysis tubing bag.To do this, you will use either indicator strips or IKI (potassium Iodide or Lugol’s solution)Data (color) will be recorded in a table
5Lab #1 – Part 2Fill dialysis bags with different concentrations of sucrose solution, place bags in beaker with water.0.0 M0.2 M0.4 M0.6 M0.8 M1.0 MWeigh each (dialysis bag + sucrose solution) BEFORE and AFTER to calculate Percent Change in MassMake line graph
6Lab#1 – Part 2 Why do we use sucrose solution? What would change if we used a salt solution?Which bags will increase in mass?Which bags will decrease in mass?What is responsible for the increase/decrease in mass?
7Lab #1 – Part 3Pieces of potato (or other vegetable) placed in various sucrose solutionsWhat sucrose concentrations we will use?Measure mass of potato before being placed in solutions to calculate Percent Change in MassMust be left Overnight.
8Lab#1 – Part 3 Why do we use sucrose solution? Which potato samples will increase in mass?Which potato samples will decrease in mass?What is responsible for the increase/decrease in mass?
9Lab #1 – Part 4 Calculate Water Potential Water potential = Solute potential + Pressure potentialΨ = Ψs + ΨpΨs = -iCRTi = 1C = Molar ConcentrationR = (liter*bar)/(mole*K)T = temperature in Kelvin
10What to do next? Organize yourselves into groups of 3-4 Each group is responsible for getting the following:12 clear or translucent plastic cups (10oz or more)Sharpie Permanent MarkerLabel CupsName of group + Period2 sets of (0.0M, 0.2M, 0.4M, 0.6M 0.8M 1.0M)
12Lab #2 – Enzyme Kinetics We will do a modified version of Lab #2 ToothpickaseSelf-Contained Lab ExerciseSeparate yourself into groups of 3-4Need:Approximately 600 flat toothpicksApproximately 100 round or reinforced toothpicksOnly need to be harder to break than the flat toothpicks
13Lab #3 – Mitosis & Crossing Over Virtual Lab ExerciseData Group Assignment (1-8) by teacher.First, identify cells in various stages of Mitosis (including interphase)Second, calculate map units and draw conclusions
14Lab #4 - Photosynthesis 2nd Actual Lab 2 Parts Fingers Crossed? Chromatography (Semi-FAIL)Part that fails miserablyFingers Crossed?IF worse comes to worse, you will just analyze given data