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Analysis of Phenotypic Data Using PM Software File Management Parametric Analysis v. 1.2.02 Michael Ziman, Ph.D. Director, PM Services.

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Presentation on theme: "Analysis of Phenotypic Data Using PM Software File Management Parametric Analysis v. 1.2.02 Michael Ziman, Ph.D. Director, PM Services."— Presentation transcript:

1 Analysis of Phenotypic Data Using PM Software File Management Parametric Analysis v Michael Ziman, Ph.D. Director, PM Services

2 Overview File Management Version/Replicate Analysis –Organize OmniLog data –View replicate and average graphs –Export raw data replicates Parametric Version/Pairwise Comparison –View replicate and average graphs –Create Pairwise Comparison –Export average parameters

3 File Management/Kinetic Plot

4 Data Organization: by assay or filter

5 PM1.okaPM11.oka PM2.okaPM12.oka PM3.okaPM13.oka PM4.okaPM14.oka PM5.okaPM15.oka PM6.okaPM16.oka PM7.okaPM17.oka PM8.okaPM18.oka PM9.okaPM19.oka PM10.okaPM20.oka PM1.okaPM11.oka PM2.okaPM12.oka PM3.okaPM13.oka PM4.okaPM14.oka PM5.okaPM15.oka PM6.okaPM16.oka PM7.okaPM17.oka PM8.okaPM18.oka PM9.okaPM19.oka PM10.okaPM20.oka File Management Reproducibility Analysis Flowchart Average Replicates Display and Analyze Reproducibility

6 Selected Files in Data List

7 Reproducibility Analysis

8 Export Kinetic Data

9 Raw Data Replicates

10 PM1.okaPM11.oka PM2.okaPM12.oka PM3.okaPM13.oka PM4.okaPM14.oka PM5.okaPM15.oka PM6.okaPM16.oka PM7.okaPM17.oka PM8.okaPM18.oka PM9.okaPM19.oka PM10.okaPM20.oka PM1.okaPM11.oka PM2.okaPM12.oka PM3.okaPM13.oka PM4.okaPM14.oka PM5.okaPM15.oka PM6.okaPM16.oka PM7.okaPM17.oka PM8.okaPM18.oka PM9.okaPM19.oka PM10.okaPM20.oka MUT.dlb WT.dlb File Management Parametric Analysis PairwiseComparisonPairwiseComparison Pairwise Comparison Flowchart

11 Parametric Version: Load Data List(s)

12 Calculate Parameters

13 Lag Average Height = sum of reads divided by number of reads dX dY

14 TEST vs REFERENCE ~2000 Kinetic Curves Defects in Tryptophan Metabolism

15 Report

16 Export Parameters

17 Average Parameters or Paramter Difference Values


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