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Analysis of Phenotypic Data Using PM Software

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Presentation on theme: "Analysis of Phenotypic Data Using PM Software"— Presentation transcript:

1 Analysis of Phenotypic Data Using PM Software
File Management Parametric Analysis v Michael Ziman, Ph.D. Director, PM Services

2 Overview File Management Version/Replicate Analysis
Organize OmniLog data View replicate and average graphs Export raw data replicates Parametric Version/Pairwise Comparison Create Pairwise Comparison Export average parameters

3 File Management/Kinetic Plot

4 Data Organization: by assay or filter

5 Reproducibility Analysis Flowchart
PM1.oka PM11.oka PM2.oka PM12.oka PM3.oka PM13.oka PM4.oka PM14.oka PM5.oka PM15.oka PM6.oka PM16.oka PM7.oka PM17.oka PM8.oka PM18.oka PM9.oka PM19.oka PM10.oka PM20.oka Display and Analyze Reproducibility File Management Average Replicates PM1.oka PM11.oka PM2.oka PM12.oka PM3.oka PM13.oka PM4.oka PM14.oka PM5.oka PM15.oka PM6.oka PM16.oka PM7.oka PM17.oka PM8.oka PM18.oka PM9.oka PM19.oka PM10.oka PM20.oka

6 Selected Files in Data List

7 Reproducibility Analysis

8 Export Kinetic Data

9 Raw Data Replicates

10 Pairwise Comparison Flowchart
PM1.oka PM11.oka PM2.oka PM12.oka PM3.oka PM13.oka PM4.oka PM14.oka PM5.oka PM15.oka PM6.oka PM16.oka PM7.oka PM17.oka PM8.oka PM18.oka PM9.oka PM19.oka PM10.oka PM20.oka MUT.dlb File Management Parametric Analysis Pairwise Comparison PM1.oka PM11.oka PM2.oka PM12.oka PM3.oka PM13.oka PM4.oka PM14.oka PM5.oka PM15.oka PM6.oka PM16.oka PM7.oka PM17.oka PM8.oka PM18.oka PM9.oka PM19.oka PM10.oka PM20.oka WT.dlb

11 Parametric Version: Load Data List(s)

12 Calculate Parameters

13 dX dY Average Height = sum of reads divided by number of reads Lag

14 TEST vs REFERENCE ~2000 Kinetic Curves
Defects in Tryptophan Metabolism

15 Report

16 Export Parameters

17 Average Parameters or Paramter Difference Values


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