Presentation is loading. Please wait.

Presentation is loading. Please wait.

Sample & Assay Technologies - 1 - QIAgility ─ Pure Precision Andrea Tesoriero Application Specialist QIAGEN 2009.

Similar presentations


Presentation on theme: "Sample & Assay Technologies - 1 - QIAgility ─ Pure Precision Andrea Tesoriero Application Specialist QIAGEN 2009."— Presentation transcript:

1 Sample & Assay Technologies QIAgility ─ Pure Precision Andrea Tesoriero Application Specialist QIAGEN 2009

2 Sample & Assay Technologies First we automated manual spin column preps… You Have More Important Things To Do…. Now we are able to automate all manual pipetting in the lab… Now QIAGEN takes the next step and enables another convenience shift

3 Sample & Assay Technologies From Manual to Automation Higher Precision and more time every day for… Preparing & designing new experiments Data analysis Literature search Writing scientific articles And much more… Eliminating tedious pipetting in the lab!

4 Sample & Assay Technologies Precision Results achieved exceed the best hand pipetting Versatility Supports all different cycler formats in the market Ease-of-use What-you-see-is-what-you-get software interface Speed Streamlined workflow by Developed For PCR Setup QIAgility Main Advantages

5 Sample & Assay Technologies QIAgility performs all “tricks” an experienced technician uses while pipetting Dynamic liquid level sensing limiting tip immersion  Tracking the meniscus in each tube to minimize tip retention Retracting the tip while dispensing  Minimizing Tip/Liquid contact Slowing down pipetting speed for viscous liquids  Avoiding formation of bubbles in tip Selecting automatically which tip (50µl or 200µl) to select  maximizing precision High Precision Meets Pipetting Skills Precision Aspects Precision

6 Sample & Assay Technologies No matter what real-time instrument you use, QIAgility is compatible The system is pre-configured to work with all PCR tube types and all plate formats, including varieties of 96- well, 384-well and Rotor-Disc Beside any PCR setup the system can perform  Set-up of quantification standards (dilution series)  Normalization of sample concentration  Sample pooling  Transfer of samples from one tube format to another  Automatic set up for multiple variables  Post PCR setup The Hallmark Of QIAgility Is Its Versatility Versatility Aspects Versatility

7 Sample & Assay Technologies Versatility QIAgility supports all real-time PCR formats LightCycler™ Capillaries Rotor-Gene™ 0.2 mL, 0.1 mL and Gene-Disc™ formats SmartCycler™ Tubes Roche COBAS ™ 96-well and 384-well (Incl. all ABI, Bio-Rad, Stratagene, Eppendorf etc. instruments)

8 Sample & Assay Technologies Versatility 96 and 384-well support Compatible with plates, tubes, tube racks, cooling blocks etc

9 Sample & Assay Technologies Versatility Unusual formats are also supported Including: MALDI plates, ELISA plates, reservoir plates, etc, etc

10 Sample & Assay Technologies The Intuitive Software Is Easy To Use Software Features The software mirrors the actual deck setup Intuitive layout—simple to learn Calculation of reagent needs according to the number of reactions and replicates specified Tip re-use options Import/Export of sample names Pre- and Post-run reports including worktable setup guide Virtual mode aids new protocol testing Wizards for speedy PCR setup and optimization Ease of Use

11 Sample & Assay Technologies You Have More Important Things To Do…. QIAgility Means Time Savings “If you worked once with the QIAgility, you don’t remember how you have managed without…” Staff can be attending to other duties while the QIAgility is running  Simple Transfer Actions 1:1 –96 samples 17 minutes 6 minutes with tip reuse  Transfer Actions with pre-MM –96 samples PCR set-up ~25 minutes  Transfer Actions with pre-MM –192 samples PCR set-up ~46 minutes Speed

12 Sample & Assay Technologies Unrivaled Pipetting Precision RotorGene Probe RT PCR Kit Data presented in brochure table 1 Precision

13 Sample & Assay Technologies Exceptional Reproducibility and Accurate Results RotorGene Probe PCR – Template dilutions Setup Details  Target: IL1 R2  Sample: Hs gDNA  10 twofold dilutions (30ng – 60pg)  5 replicates  Rotorgene 6000 Data presented in brochure figure 1 Precision

14 Sample & Assay Technologies Automated Workflow for Accurate Results RotorGene Probe RT PCR Kit Setup Details  Target: IL1 2RB1  Sample: Leuko RNA  Standard row in triplicates  5 tenfold dilutions (10 pg – 100 ng)  Rotorgene 6000 Data presented in brochure fig 3 Precision

15 Sample & Assay Technologies Worktable setup example Worktable Positions 1.Master Mix Block 2.Reagent Block 3.Tips 4.Tips 5.Tips or Rotor-Gene Q Loading Block 6.Tips 7.Sample Plate or Rotor-Gene Q Loading Block 8.Reaction Plate

16 Sample & Assay Technologies Guides you through creating a run file with master mix, standard, Sample and final reaction! Life Can Be So Simple…. QIAgility PCR Setup Wizard

17 Sample & Assay Technologies Just a few simple steps Define Plate/Tube Format Sample Bank (number of samples) Reagents (primer, buffer, taq, etc.) Master Mix (pre-mix, setup) Standards Reactions  Add Controls  Add Standard  Add Samples GO! Set Up Your Own Run Software Explanation

18 Sample & Assay Technologies ̣ View the software


Download ppt "Sample & Assay Technologies - 1 - QIAgility ─ Pure Precision Andrea Tesoriero Application Specialist QIAGEN 2009."

Similar presentations


Ads by Google