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Derrick R. J. Kolling March 16, 2010 Potential Algal Biofuel Applications in West Virginia Derrick R. J. Kolling Department of Chemistry STaR Symposium.

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Presentation on theme: "Derrick R. J. Kolling March 16, 2010 Potential Algal Biofuel Applications in West Virginia Derrick R. J. Kolling Department of Chemistry STaR Symposium."— Presentation transcript:

1 Derrick R. J. Kolling March 16, 2010 Potential Algal Biofuel Applications in West Virginia Derrick R. J. Kolling Department of Chemistry STaR Symposium October 23, 2013

2 Derrick R. J. Kolling October 23, 2013 Talk Overview Biofuels overview –Why do we need biofuels? The light reactions of photosynthesis 2H 2 O + 4h  4H + + 4e - + O 2 Tools of the trade –O 2 electrode –Fluorometer –EPR spectrometer –GC-MS Research projects –OEC photoassembly –Temperature dependence of molecular photosynthesis –Algal lipidomics –Biofuels from invasive algal species

3 Renewable Energy Biofuels Solar power Wind power Tidal power Hydropower Geothermal futurefarmers.com/survey/algae epmb.berkeley.edu

4 Derrick R. J. Kolling October 23, 2013 Dismukes et al. (2008)

5 Derrick R. J. Kolling October 23, 2013

6 Photosynthesis Derrick R. J. Kolling March 16, 2010 Carbon Fixation Electrons (as NADH, NADPH, Reduced Ferredoxin) Biomass CO 2 H2OH2O O2O2 Protein Synthesis e-e- ATP Source: Damian Carrieri anaerobic Fermentation O2O2 H2H2 lipid protein carbohydrate biodiesel animal feed ethanol CO 2, organic acids ATP Lipid Synthesis H2OH2O

7 Derrick R. J. Kolling October 23, 2013 Dismantling photosynthesis from the organism to the molecules…. Light Reactions

8 Derrick R. J. Kolling October 23, 2013 Barber & Iwata (2004) Science …from the molecular to the atomic level Umena et al. (2011) Nature Light Reactions

9 Derrick R. J. Kolling October 23, 2013 Research Tools Oximetry Fluorescence GC-MS Electron Paramagnetic Resonance

10 Temperature dependence of oxygen evolution PSII PSII w/DCBQ PSII normalized OEC Photoassembly

11 Temperature dependence of oxygen evolution vs. photoassembly Photoassembled PSII at 28°C Intact PSII - Share a rate-limiting step

12 OEC Photoassembly -Protease activity explains dip

13 OEC Photoassembly Findings Oxygen evolution and OEC photoassembly share a 28°C maximum OEC photoassembly and PSII oxygen evolution appear to share a rate-limiting step Thermal inactivation of PSII and apo-PSII occurs at temperatures greater than 28°C Thermal inactivation is partially due to protease activity (Deg and/or FtsH?)

14 OEC Photoassembly Application www3.imperial.ac.uk Artificial Leaf Bioinspired/biomimicked System

15 Algal Lipidomics Comparison of lipid accumulation in photomixotrophically and heterotrophically grown Chlorella vulgaris cultures

16 Algal Lipidomics [[ [ [ [ [[[ [ Culture Measured: -[Chl a]-turbidity -dry weight-lipid dry weight

17 Algal Lipidomics Cell Density 750nm Chl a Expression Derrick R. J. Kolling October 23, 2013

18 Algal Lipidomics Dry Weight Growth Curves and Corresponding Dry Lipid Weights C 18:2(n-6): linoleic acid C 18: stearic acid C 18:1(n-9): oleic acid C 16: palmitic acid

19 Algal Lipidomics Photomixotrophically grown cells produce 2X as many lipids as do heterotrophically grown cells Photomixotrophically grown cells reach stationary phase and higher biomass sooner Cells produce palmitic, stearic, oleic, and linoleic acids Findings

20 Algal Lipidomics Application Chisti (2007) Biotech. Advances

21 Derrick R. J. Kolling October 23, 2013 Temperature Dependence of PSII -James Board, Hope Cook, Matt Thompson, Shane Kagen, Jordan Hilgeford, Justin Erwin OEC photoassembly -James Board, Hope Cook, Ben Blodgett, Matt Thompson, Shane Kagen, Jordan Hilgeford, Chase Turner, Ben Weiner Algal lipidomics -Ben Woodworth, Tony Stephenson, Rebecca Mead, Courtney Nichols, Prof. Leslie Frost (MU) Bioethanol from invasive algal species -Kevin Militello, Shaheed Elhamdani Acknowledgements AcknowledgementsAcknowledgements Funding This material is based upon work supported by the National Science Foundation under Cooperative Agreement Award number EPS and CHE


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