Presentation on theme: "Winston Sanders. Mu Opioid Receptor (MOR) Stimulus Trafficking What if the unwanted side effects of a drug could be diminished while its medicinal."— Presentation transcript:
Mu Opioid Receptor (MOR) Stimulus Trafficking What if the unwanted side effects of a drug could be diminished while its medicinal effects amplified?
Bordetela pertussis toxin (PTX) -rendered PTX-sensitive Ga (subunit) proteins unresponsive to GPCR activation -allowing focused aim towards specific PTX-insensitive mutant Ga proteins -Interferes with natural communication b/w GPCR and G-protein -Ineffective Mechanism Examining the Pathways
The Experiment Inactive Active!
How could desired pathway be identified? Interaction of the Xanthine Nucleotide Binding Goa Mutant with G Protein-coupled Receptors Double Mutant GoaX The Experiment (con’t)
What were results of GoaX when compared to wild-type a-subunit? Activates in same manner! GoaX conserves natural responses The Experiment (con’t)
Use GoaX xanthine binding Ga subunits as efficient indicators Individually transfect specific X-mutant Ga subtypes with the receptor of interest (MOR) Successfully study individual activation pathways Future Hopes