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Development of Bleaching Reagent Testing by Using Mangosteen Peel Extract Solution 1.

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Presentation on theme: "Development of Bleaching Reagent Testing by Using Mangosteen Peel Extract Solution 1."— Presentation transcript:

1 Development of Bleaching Reagent Testing by Using Mangosteen Peel Extract Solution 1

2 Members Nathida Wiriyachoke No.2 M.4/1 Chanon Pinyopawasutti No.11 M.4/1 Nuttanon Jantanapornchai No.13 M.4/1Advisor Dr. Usa Jeenjenkit 2

3 Inquiry questions How the mangosteen peel extract solution identify the concentration of bleaching reagent ? Can mangosteen peel extract identify the concentration of bleaching reagent ? 3

4 Introduction 4

5 Bleaching reagent Sodium Metabisulphite (Na 2 S 2 O 5 ) Sodium hydrosulfite (NaHSO 4 ) Sodium hypocloride (NaOCl) 5

6 The benefit of bleaching reagent Dying industry : Making new colors Food industry : making food more attractive 6

7 Disadvantage Frequently use of bleach would effect to the respiratory symptoms (Zock et. al., 2009). pain-and-lower-back-pain.html 7

8 One out of 560 larvae treated with BW [bleach solution] (97.3% of the animals were uninfected) was recovered as an adult worm (Zanini & Graeff-Teixeira, 2001). 8

9 Bleach causes a chemical reaction in the gill tissue which blocks the transfer of oxygen and causes the lobster to slowly asphyxiate ( T. Wilson, Vaughan, K. Wilson,Simon & Lockhart, 2007). 9

10 Scope Bleaching reagent : sodium metabisulphite Bleaching reagent concentration :1, 5, 50 and 100 ppm Dilution ratio of mangosteen extract solution :1:120, 1:160 and 1:200 10

11 Mixture ratio of bleaching reagent and mangosteen extract : 1:2 Wavelength of spectrophotometer : 410 nm 11

12 Materials & Method 12

13 Prepare bleaching reagent solution. Na 2 S 2 O g H 2 O 1 liter Na 2 S 2 O 5(aq) 100 ppm 13

14 Dilute bleach solution Na 2 S 2 O 5(aq) 5 ml 100 ppm H 2 O 5 ml Na 2 S 2 O 5(aq) 50 ppm 14

15 Mix the extract solution and different concentration of bleaching solution by 2:1 (v/v). 15

16 Measure the absorbance by using spectrophotometer at wavelength 410 nm. 16

17 Results 1:120 of Mangosteen peel extract : water 17

18 1:160 of Mangosteen peel extract : water (First time) 18

19 1:160 of Mangosteen peel extract : water ( Second time) 19

20 1:200 of Mangosteen peel extract : water 20

21 The effect of bleach solution with mangosteen extract Concentration of bleach solution (ppm ) Absorbance value 21

22 Conclusion Mangosteen peel extract solution can identify the concentration of bleaching reagent. 22

23 Discussion The color of mangosteen extract solutions were clearer when we put the bleaching solutions. The higher concentration of bleaching solutions the lower absorbance. 23

24 0 ppm 1 ppm 10 ppm 50 ppm 100 ppm Picture shown the mixture of bleach solution and mangosteen peel extract 24

25 The plots of all ratios between mangosteen extract solution and bleaching solution have the same trend. We use the absorbance value for drawing graph instead of the others because it is normally use. 25

26 Benefit We can use mangosteen extract solution to identify the concentration of bleach reagent by natural substance. 26

27 Future research Testing other kinds of bleach reagent such as sodium hydrosulphite instead of sodium metabisulphite. Develop paper testing for bleaching reagent like Garnier paper. 27

28 References Wilson, D. T., Vaughan, D., Wilson, S. K., Simon, C. N. & Lockhart, K. ( April 2008). Efficacy of a starch-iodine swab technique to detect the illegal use of bleach in a Spiny Lobster (Panulirus argus) fishery. Fisheries Research. Volume 90, Issues 1-3, Pages Retrieved from cle/pii/S Zanini, G.M., & Teixeira, C. G.(15 January 2001). Inactivation of infective larvae of Angiostrongylus costaricensis with short time incubations in 1.5% bleach solution, vinegar or saturated cooking salt solution. Acta Tropic. Volume 78, Issue 1, Pages Retrieved from cle/pii/S X

29 Zock, J.P. et. al. (October 2009). Domestic use of hypochlorite bleach, atopic sensitization, and respiratory symptoms in adults. Journal of Allergy and Clinical Immunology. Volume 124, Issus 4, Page Retrieved from ticle/pii/S

30 Acknowledgements Dr. Usa Jeenjenkit and our SINOS teachers Chemistry Department and Biology Department of Mahidol Wittayanusorn School 30

31 Thank you for your attention 31


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