We think you have liked this presentation. If you wish to download it, please recommend it to your friends in any social system. Share buttons are a little bit lower. Thank you!
Presentation is loading. Please wait.
Published byKennedy Stoakes
Modified over 2 years ago
De androgeenreceptor is een transcriptiefactor
Androgens AR Δ Gene expression A A
Androgens affect: - Embryologic development - Bone and muscle - Male puberty - Behaviour - Reproduction - Prostate & PrCa - Osteoporosis and frailty Androgens Testosterone Dihydrotestosterone
Androgens AR Δ Gene expression A A
Gene regulation by testosterone Testosteron binds the androgen receptor The receptor binds DNA The receptor activates gene expression Therapies
DNA binding Ligand-bindingGene activation AF1 LBD AF2 AR: DBD The receptor binds testosterone
Testosterone binds the receptor
Effect of testosterone binding H1 H2 H3 H4 H5 6 H7 H8 H9 H10 H11 H12 S1 S2 no ligand H9 H1 H2 H3 H4 H5 H7 H8 H10 H11 H12 6 T + ligand LxxLL
Gene regulation by testosterone Testosteron activates the androgen receptor The receptor binds DNA The receptor activates gene expression Therapies
The DNA-binding domain of the AR AF1 LBD AF2 AR: DBD
DNA binding Ligand-binding How does testosterone affect gene expression? Gene activation AF1 LBD AF2 AR: DBD
Gene regulation by testosterone Testosteron binds the androgen receptor The receptor binds DNA The receptor activates gene expression
Histon code recognition erasing TIF1β Histon code and testosteron action CBP, P/CAF CARM1 Histon code writing p160 RNApol II recruitment expression TFIIF Tau-1 LBD AF2 AR: DBD Tau-5 Chromatin-remodeling complex
-CRC -TIF1b Testosteron effects on the histon code: a cartoon AR -p160 RNA polII -TFIIF
DNA binding Ligand-binding The DBD and LBD communicate Transactivation Tau-1 LBD AF2 AR: DBD Tau-5 Cycling partners Cycling histon code
Gene regulation by testosterone Testosteron binds the androgen receptor The receptor binds DNA The receptor activates gene expression Prostate cancer
6,6 x 10 9 bp: A,C,G or T 10 7 Single nucleotide polymorphisms Gene fusions New markers in prostate cancer
Gene fusions in PCa CTRL : Oncogenes 50% !
Two SNPs in the TM-enhancer SNP2 SNP1 T A TM-ARE2SNP1SNP2 Chr 21: TMPRSS2 gene
Two SNPs in TM-ARE2 WTSNP1SNP2 TM-ARE2 SNP2 SNP1 T A Chr 21
Relevance of TM-ARE2 SNPs?
Mol Endo Lab AR stuctuur en functie PrCa resistentie tegen AR antagonisten Nieuwe AR antagonisten PrCa genomen en diagnostische merkers SNPs Frailty: osteoporosis
Guidelines for Field Veterinarians and Para-professionals on the Surveillance of Avian Influenza KOIKE I. (Technical Consultant) 2007.
Copyright © 2009 Pearson Education, Inc. Regulation of Gene Expression in Eukaryotes Chapter 17 Lecture Concepts of Genetics Tenth Edition.
Tutorial on 2D-NMR: Using COSY & HSQC to Assist in Assigning 1 H & 13 C NMR Spectra.
Mutational analysis of 35 bladder cancer cell lines using RNA-seq.
Copyright © 2009 Pearson Education, Inc. Art and Photos in PowerPoint ® Concepts of Genetics Ninth Edition Klug, Cummings, Spencer, Palladino Chapter 18.
HOSTEL ALUMNI TEAM STEWARDSHIP HATS A Grass Roots Effort CONNECT WITH YOUR HOSTEL & STUDENTS.
ENGG1100 Ch7: Introduction To Engineering Design (Digital Logic) Part 3 of digital logic KH WONG ENGG1100. Ch7-Digital Logic (part 3) v3h1.
MCB 317 Genetics and Genomics MCB 317 Topic 10, part 3 A Story of Transcription.
HEALTH AT WORK NETWORK STOCKTAKE 21 October 2014 Geoff Dessent Deputy Director, Health and Wellbeing.
Introduction to Molecular Cell Biology Transcription Regulation Dr. Fridoon Jawad Ahmad HEC Foreign Professor King Edward Medical University Visiting Professor.
Estrogen Receptor in Fibromatosis 1 Andrea T. Deyrup, M.D., Ph.D., 2 Maria Tretiakova, M.D., Ph.D. and 2 Anthony G. Montag, M.D. 1 Emory University,
1 and 3 November, 2006 Chapter 17 Regulation in Eukaryotes.
Lecture12 - Based on Chapter 18 - Regulation of Gene Expression in Eukaryotes I Copyright © 2010 Pearson Education Inc.
Gene expression in eukaryotes 1. Eukaryotic RNA polymerases 2. Regulation of eukaryotic RNP 3. Hormonal regulation 4. Histone acetylation.
AreaSurvey SourceNumber of items Cognitive Absorption (CA)Agarwal and Karahanna, 2000)20 questions Computer Self-Efficacy (CSE)Compeau and Higgings (1995)10.
Lecture 3. Felsenfeld & Groudine, Nature 2003 H2A, H2B, H3 and H4.
NATIONAL RESILIENCE UPDATE NATIONAL RESILIENCE UPDATE 2.
DNA, genes and chromosomes Revise your BI1505/7 notes Textbook - Purves chapters on Chromosomes… and DNA…, Lehninger chapter 24 These 3 lectures will cover.
1 Eukaryotic Gene Regulation April 24, 2015 Richard D. Howells, PhD MSB E-643
An overview of the National Performance Measures 118 NOVEMBER 2011 THE CORPORATION FOR NATIONAL & COMMUNITY SERVICE.
Caption N-terminal domain A/B domain: ligand-independent domain AF1: Activation Function 1 DBD: DNA Binding Domain LBD: Ligand Binding Domain: ligand-dependent.
Molecular Basis for Relationship between Genotype and Phenotype DNA RNA protein genotype function organism phenotype DNA sequence amino acid sequence transcription.
Control of Gene Expression Chapter 16. Contolling Gene Expression What does that mean? Regulating which genes are being expressed transcribed/translated.
Regulation of transcription in eukaryotes. Regulation of transcription is usually complex in eukaryotes.
Biochemistry Sixth Edition Chapter 31 The Control of Gene Expression Part II: Eukaryotes (cis vs. trans) Copyright © 2007 by W. H. Freeman and Company.
Exon selection factor Exon selection factor U2 snRNPU1 snRNP Intron 1 Overview of mRNA Splicing Exon 1 AGGU Exon 2 A AGG Factors such as U1 and U2 snRNP.
Chapter 11 Review. Explain the difference between each of the following 1. Operator, promoter -Operator: DNA segment where an inhibitor protein binds.
Sigma-aldrich.com/cellsignaling Modular Structure of Transcription Factors.
20,000 GENES IN HUMAN GENOME; WHAT WOULD HAPPEN IF ALL THESE GENES WERE EXPRESSED IN EVERY CELL IN YOUR BODY? WHAT WOULD HAPPEN IF THEY WERE EXPRESSED.
Different Strategies for Activating Transcription Factors.
The Code of Life: Topic 4 Regulation of gene expression.
Mechanism of activation. Models for mechansim of activation Direct contact between an activator and RNA polymerase or GTF Indirect interactions –Adaptor.
Cell Cycle & Cell Cycle Control. 6. Cell Cycle and Cell Cycle Control 6.1 Phases of Cell Cycle 6.2 Special Apparatus of Mitosis Chromatin/Chromosome.
Introduction to Bioinformatics II Lecture 8 By Shumaila Azam.
BIO409/509 Cell and Molecular Biology. You don’t need to hand in corrected answers for Exam #2.
Gene Expression. Cell Differentiation Cell types are different because genes are expressed differently in them. Causes: Changes in chromatin structure.
IN CANCER MOLECULAR DETECTION. WHAT DO THEY DETECT? Specific proteins Expression of certain genes Mutations Epigenetic Changes.
Hormone Action Brian Feldman, MD, PhD Department of Pediatrics/ Endocrinology
Gene regulation in cancer 11/14/07. Overview The hallmark of cancer is uncontrolled cell proliferation. Oncogenes code for proteins that help to regulate.
Inquiry into Life Twelfth Edition Chapter 25 Lecture PowerPoint to accompany Sylvia S. Mader Copyright © The McGraw-Hill Companies, Inc. Permission required.
Jeopardy Heading1Heading2Heading3Heading4 Heading5 Q $100 Q $200 Q $300 Q $400 Q $500 Q $100 Q $200 Q $300 Q $400 Q $500 Final Jeopardy.
Chapter 11 Molecular Mechanisms of Gene regulation Jones and Bartlett Publishers © 2005.
Recombination breakpoints Family Inheritance Me vs. my brother My dad (my Y)Mom’s dad (uncle’s Y) Human ancestry Disease risk Genomics: Regions mechanisms.
Integrative Genomics. Double-helix DNA strands are separated in the gene coding region Which enzyme detects the beginning of a gene ? RNA Polymerase (multi-subunit.
The Efficacy of Synthetic Steroids to Inhibit Hormonal Receptors Malik D. Lewis Howard University Department of Chemistry
Lecture #8Date _________ n Chapter 19~ The Organization and Control of Eukaryotic Genomes.
Regulation of gene expression Fall, Gene Expression Regulation in Prokaryotes it includes : Control of transcription, little on translation How.
1 Control of eukaryotic gene expression As usual, much more complicated than in prokaryotes. –Increased amount of DNA –Tight packing into nucleosomes –Physical.
Tutorial 10 Question 2a, 2b, 2c by Kapilesh Ramesh.
© 2017 SlidePlayer.com Inc. All rights reserved.