2 What is an enzyme?A protein found in organisms with a specific function.Examples: Protease breaks down proteinsLipase breaks down lipidsAmylase breaks down carbsWhere do you think these enzymes are found?What is similar between the 3 enzymes?
3 Function of EnzymesReduce the amount of activation energy needed to start a chemical reaction. As a result the enzyme acts as a catalyst by speeding up the reaction.Ex. Digestion would take a lot longer w/o enzymes!Control very specific reactions (see lock and key model)Breakdown reactions, ex. DigestionSynthesis reactions, ex. Protein Synthesis (making of proteins from ?????)
4 Progress of the reaction Course ofreactionwithoutenzymeEAwithoutenzymeEA withenzymeis lowerReactantsCourse ofreactionwith enzymeFree energyChange in free energy(∆G ) is unaffectedby enzymeProductsProgress of the reaction
5 Lock & Key Model Enzymes are substrate specific! Substrate Observe the picture above. Provide evidencethat supports the claim that enzymes aresubstrate specific.Enzyme
7 Purpose of this lab! (See NTK Box, p. 370-71) Your team will investigate the role of amylase in breaking down starch into smaller molecules called maltose and glucose.How do different variables affect enzyme reactions?
9 Guidelines You will be observing: The amount of glucose produced. The presence of starch.You will collect data:0 minutes4 minutesYou must use both amylase and starch, but change only 1 variable!Note: You are doing this for all 3 concentrations, temperatures, or pH’s