2What is an enzyme?A protein found in organisms with a specific function.Examples: Protease breaks down proteinsLipase breaks down lipidsAmylase breaks down carbsWhere do you think these enzymes are found?What is similar between the 3 enzymes?
3Function of EnzymesReduce the amount of activation energy needed to start a chemical reaction. As a result the enzyme acts as a catalyst by speeding up the reaction.Ex. Digestion would take a lot longer w/o enzymes!Control very specific reactions (see lock and key model)Breakdown reactions, ex. DigestionSynthesis reactions, ex. Protein Synthesis (making of proteins from ?????)
4Progress of the reaction Course ofreactionwithoutenzymeEAwithoutenzymeEA withenzymeis lowerReactantsCourse ofreactionwith enzymeFree energyChange in free energy(∆G ) is unaffectedby enzymeProductsProgress of the reaction
5Lock & Key Model Enzymes are substrate specific! Substrate Observe the picture above. Provide evidencethat supports the claim that enzymes aresubstrate specific.Enzyme
7Purpose of this lab! (See NTK Box, p. 370-71) Your team will investigate the role of amylase in breaking down starch into smaller molecules called maltose and glucose.How do different variables affect enzyme reactions?
8Variables (circle one) Amylase ConcentrationStarch ConcentrationTemperaturepH (acidic or basic environment)
9Guidelines You will be observing: The amount of glucose produced. The presence of starch.You will collect data:0 minutes4 minutesYou must use both amylase and starch, but change only 1 variable!Note: You are doing this for all 3 concentrations, temperatures, or pH’s