Hydrolysis DNA replication Recombinant DNA formation Genetic screening
Recombinant DNA formation
The bacterial cell to produce a human protein The cloning of the human that donated that DNA fragment Humans to become immune to an infection by this type of bacteria The cloning of this type of bacteria
The bacterial cell to produce a human protein
Using radiation to trigger mutations Genetic mapping of bacterial DNA to activate the gene for insulin production Deleting many DNA segments from bacterial DNA Inserting a portion of human DNA into the ring-shaped DNA of bacteria
Act as a template for repeated replication
There are many ethical problems involved in cloning humans The technology to clone humans has not been explored Human reproduction is very different from that of other mammals Cloning humans would take too long.
Change the pH of the soil Plant seeds of a different kind of plant Move the tomato plants to an area with less sunlight Reduce the amount of water available to the plant
Change the pH of the soil
Field of vision is smaller under low power than under high power Field of vision is larger under low power than under high power Specimen does not need to be stained for observation under low power but must be stained for observation under high power Portion of the specimen that can be observed under low power is less than the portion that can be observed under high power.
Field of vision is larger under low power than under high power
Transformation enzyme Restriction enzyme DNA ligase DNA polymerase Nuclease enzyme
Is ready for clinical testing to begin. Is being produced on a large scale by the manufacturing department Has been determined safe and effective Needs to be reviewed regularly with a comprehensive product development plan.
Needs to be reviewed regularly with a comprehensive product development plan
Universities Medical clinics Companies Government agencies
Engage in “pure science” Cure all the diseases of the world Publish results in scientific journals Provide a product of service that is useful to society and results in earnings
Different cell lines (CHO & HeLa cells) are more suited to producing certain kinds of recombinant proteins. Prokaryotic cells specialize in certain tasks and can therefore grow more rapidly than eukaryotic cells. There is great cell variety in multicellular organisms in which different kinds of cells have different jobs to do. All of the above
There is great cell variety in multicellular organisms in which different kinds of cells have different jobs to do
µg/µL mg/mL µM All of these
The proportion of solute to solvent increases The proportion of solvent to solute increases The normality of the solution shifts Re-ionization begins to occur
Break the phosphodiester bonds between nucleotides Break open the nuclear membrane Break apart the cellulose in the plant cells. Break apart the cell membrane.
Break apart the cellulose surrounding plant cells.
Bacteria Yeast Chinese hamster ovary cells All of the above
P-1000 P-200 P-20
DNA ligase DNA helicase DNA emporase Restriction enzymes
14X 400X 40X 4X
Water Gravity Salt Electricity
Kill the bacteria. Make the DNA glow. Separate the DNA strands. Move the DNA in the gel.
Separate the DNA strands
The strands cross each other The strands run parallel to each other but run in opposite directions The strands run the same directions and are parallel Strands run at right angles to each other
The strands run parallel to each other but run in opposite directions
Restriction endonuclease RNA polymerase DNA polymerase DNA ligase