Presentation on theme: "ANTIGEN ANTIBODY REACTION"— Presentation transcript:
1ANTIGEN ANTIBODY REACTION ByDr. Emad AbdElhameed MoradLecturer of Medical Microbiology and Immunology
2ELISA It is an abbreviation of enzyme linked immunosorbent assay. Types:IndirectDirectFor the antigenFor the antibody
3Indirect ELISAA known antigen is fixed to a solid phase (microplate wells).The serum of the patient is then added to the wells and incubated.Washing of the wells is done.Antihuman gamma globulin labeled with a suitable enzyme is added to the wells and incubated.A suitable substrate is added and incubated.Color development in the wells means positive serum.
6ImmunofluorescenceIt is another form of immunoassays in which fluorescein labeled antibodies are used.Fluorescein is a dye which emits green fluorescence under ultraviolet light.Types:Indirect IFDirect IFFor the antigenFor the antibody
7Indirect IF A known antigen is fixed to a solid phase (slide). The serum of the patient is layered on the slide and incubated.Washing of the slide is done.Antihuman gamma globulin labeled with fluorescein dye is added to the slide and incubated.The slide is then examined under the fluorescent microscope.Positive fluorescence under the microscope means positive serum.