Presentation is loading. Please wait.

Presentation is loading. Please wait.

ANTIGEN ANTIBODY REACTION By Dr. Emad AbdElhameed Morad Lecturer of Medical Microbiology and Immunology.

Similar presentations


Presentation on theme: "ANTIGEN ANTIBODY REACTION By Dr. Emad AbdElhameed Morad Lecturer of Medical Microbiology and Immunology."— Presentation transcript:

1

2 ANTIGEN ANTIBODY REACTION By Dr. Emad AbdElhameed Morad Lecturer of Medical Microbiology and Immunology

3 It is an abbreviation of enzyme linked immunosorbent assay. Types: ELISA Direct Indirect For the antigenFor the antibody

4 o A known antigen is fixed to a solid phase (microplate wells). o The serum of the patient is then added to the wells and incubated. o Washing of the wells is done. o Antihuman gamma globulin labeled with a suitable enzyme is added to the wells and incubated. o Washing of the wells is done. o A suitable substrate is added and incubated. o Color development in the wells means positive serum. Indirect ELISA

5

6

7 It is another form of immunoassays in which fluorescein labeled antibodies are used. Fluorescein is a dye which emits green fluorescence under ultraviolet light. Types: Immunofluorescence Direct IF Indirect IF For the antigenFor the antibody

8 o A known antigen is fixed to a solid phase (slide). o The serum of the patient is layered on the slide and incubated. o Washing of the slide is done. o Antihuman gamma globulin labeled with fluorescein dye is added to the slide and incubated. o Washing of the slide is done. o The slide is then examined under the fluorescent microscope. o Positive fluorescence under the microscope means positive serum. Indirect IF

9

10

11 It is another form of immunoassays in which radiolabeled antibodies are used. The radioactivity is measured by gamma counter. Radioimmunoassay

12 GOOD LUCK


Download ppt "ANTIGEN ANTIBODY REACTION By Dr. Emad AbdElhameed Morad Lecturer of Medical Microbiology and Immunology."

Similar presentations


Ads by Google