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What is Bio-technology? Why is it important to understand your genetic make up? How does this information affect your life? Society? http://www.komonews.com/news/con sumer/17071161.html Advancement of technology for research associated with life science. Especially Genetic Engineering (changing genes and recombinant DNA (changing DNA sequences)

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Eliminating crime at EHS by incorporating electrophoresis into our discipline policy. Would you like to help? Eliminating crime at EHS by incorporating electrophoresis into our discipline policy. Would you like to help? Tools you need to learn to use… Tools you need to learn to use… Micro pipettes… Electrophoresis… Before we can use these tools we will need training on how they work…

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What is the metric system?. How big is a Liter? How big is a Liter? How big is a mili Liter? How big is a mili Liter? mL(1/1000 L) mili means one thousanth mL(1/1000 L) mili means one thousanth How big is a micro liter compared to a L and a mL? How big is a micro liter compared to a L and a mL? (1/1000 of a mL) (1/1,000,000 of a L) (1/1000 of a mL) (1/1,000,000 of a L) Which of these units do you think would be the best for looking at pieces of DNA? Which of these units do you think would be the best for looking at pieces of DNA? Why? Why? Standard measurement system used through out the world

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The MICRO LITER!! DNA IS SO SMALL THAT IT NEEDS TO BE LOOKED AT IN VERY TINY PIECES DNA IS SO SMALL THAT IT NEEDS TO BE LOOKED AT IN VERY TINY PIECES What tool should we use to measure DNA? What tool should we use to measure DNA? Micro-pipets allow us to take samples as small as.5 µL and as large as 1000 µL (1 mL) Micro-pipets allow us to take samples as small as.5 µL and as large as 1000 µL (1 mL)

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Are you a metric system master? When I convert from L to mL will the number get smaller or larger? When I convert from L to mL will the number get smaller or larger? Larger, I will move my decimal place 3 spaces to the right because it is a thousand times smaller. Larger, I will move my decimal place 3 spaces to the right because it is a thousand times smaller. When I convert from mL to µL will the number get smaller or larger? When I convert from mL to µL will the number get smaller or larger? Larger, I will move my decimal place 3 places to the right. Larger, I will move my decimal place 3 places to the right.

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Metric System Master? When I convert from µL to mL will the number get smaller or larger? When I convert from µL to mL will the number get smaller or larger? Smaller, I will move my decimal place 3 places to the left. For example 2 µL =.002 mL Smaller, I will move my decimal place 3 places to the left. For example 2 µL =.002 mL When I convert from mL to L will the number get smaller or larger? When I convert from mL to L will the number get smaller or larger? Smaller. I will move my decimal place 3 spaces to the left. Smaller. I will move my decimal place 3 spaces to the left. 2.0 mL =.002 L 2.0 mL =.002 L

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Factor Label Method A great way to set up conversions A great way to set up conversions Start the conversion with what you know and make a relationship that relates to what you want to know Start the conversion with what you know and make a relationship that relates to what you want to know For example when determining how many For example when determining how many µL are in 20 L set up this conversion µL are in 20 L set up this conversion 20L 1,000,000 µL 1 L 1 L This means 20 X 1,000,000 =20,000,000 µL

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Metric System Master??? If I have L and want µL what should I do? If I have L and want µL what should I do? Move the decimal place 6 places to the right Move the decimal place 6 places to the right Or set a conversion using factor label method Or set a conversion using factor label method

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I will give you a measurement and you must tell me how many µL I have… Use Factor Label method I will give you a measurement and you must tell me how many µL I have… Use Factor Label method 2 µL = how many L? 2 µL = how many L? 2 µL 1 L 2 µL 1 L 1,000,000 µL 2µL X 1L /1,000,000µL =.000002L.000002L 87 mL = how many µL? 87 mL = how many µL? 87mL 1,000 µL 1 mL 1 mL 87mLx 1000 µL/ 1mL 87,000 µL 87,000 µL

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Try these on your own…. 100 µL = How many L 100 µL = How many L 2,300,000 L = How many mL 2,300,000 L = How many mL 45 L = How many µL? 45 L = How many µL? 1..0001 2.2,300,000,000mL 3.45,000,000 µL

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EQUIPMENT!!! You must show DeNA locator competentence before you can join the EHS crime fighting team!! You must show DeNA locator competentence before you can join the EHS crime fighting team!! P-10range of.5-10 µL P-10range of.5-10 µL P-20 range of 2- 20 µL P-20 range of 2- 20 µL P-200range of 20-200 µL P-200range of 20-200 µL P 1000range of 200-1000 µL P 1000range of 200-1000 µL

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IF YOU USE THE WRONG PIPETTE IT WILL MAKE THEM LESS ACCURATE AND MAY DESTROY THEM..AND THEY MIGHT EXPLODE! If I want to measure 1.2 µL which pipette would I choose? If I want to measure 1.2 µL which pipette would I choose? P-10 P-10 If I want to measure 178 µL which pipette would I choose? If I want to measure 178 µL which pipette would I choose? P-200 P-200 If I want to measure 778 µL which pipette would I choose? If I want to measure 778 µL which pipette would I choose? P-1000 P-1000

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Try these on your own. Tell me the pipet for the specific measurement. Tell me the pipet for the specific measurement. 600 µL25 µL 600 µL25 µL 225 µL90 µL 225 µL90 µL 1 µL 18 µL 1 µL 18 µL 1.P-10004. p-200 2.P-10005. P-200 3.P-106. P-20

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Use Factor Label to solve the following How many micro Liters are in 27 L? How many micro Liters are in 27 L? How many mL are in 23.4 micro Liters? How many mL are in 23.4 micro Liters? How many micro Liters are in.234 L? How many micro Liters are in.234 L?

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Measure for Measure: You need Pipettes- May have to share p-10 and p- 1000 Pipettes- May have to share p-10 and p- 1000 Set of Tips for p-20/p-200 and p-10s will share p-1000 Set of Tips for p-20/p-200 and p-10s will share p-1000 Parafilm Parafilm Microtubes (3) Microtubes (3) Sample of water Sample of water

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How could we determine the components of a mystery dye mixture using electrophoreis?

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Purpose: To determine the dyes in two mystery mixtures: XXX and ZZZ Purpose: To determine the dyes in two mystery mixtures: XXX and ZZZ

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Procedure: Materials electrophoresis apparatus (BRL Horizon 58 gel box) Power Supply (shared by two groups) beaker for used tips 1% agarose gel 1X TAE buffer (enough to cover gel) 6 known dyes 2 mystery mixtures (XXX and ZZZ) micropipet tips micropipets blot paper (1 per person) ziplock bag acetate sheets (1 per person) plastic wrap marker pen ruler (mm) microtube rack microtubes

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Steps See Handout:

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Data: Distance traveled (in mm) Distance traveled (in mm) To which pole +/- To which pole +/- Comments & Observations Comments & Observations Picture of Gel Before and After

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For Tommorrow: Finish Stuff to know before you Go: In reading notebook Finish Stuff to know before you Go: In reading notebook Pre-Lab- Purpose,Procedure,Data Section Pre-Lab- Purpose,Procedure,Data Section

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How should I take data? Look at get on light box Look at get on light box Record DNA distances on acetate Record DNA distances on acetate Compare # of bands and distance of bands Compare # of bands and distance of bands

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Work on Conclusion after youre done! If you will not be here Monday/Tuesday or Wednesday you must keep up! If you will not be here Monday/Tuesday or Wednesday you must keep up! We will quiz Monday/Tuesday after break as well as have a GATTACA discussion We will quiz Monday/Tuesday after break as well as have a GATTACA discussion

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Gel Electrophoresis of DNA

Gel Electrophoresis of DNA

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