Presentation on theme: "Outline the different patterns of inheritance associated with X linked genes. Where possible give a molecular explanation for the pattern. 6th October."— Presentation transcript:
1 Outline the different patterns of inheritance associated with X linked genes. Where possible give a molecular explanation for the pattern.6th October 2008
2 X Linked InheritanceRefers to the pattern of inheritance of a condition caused by a mutations in a gene located on the X chromosome.Males have one X chromosome and a Y chromosome and females have two X chromosomes.Because of this fundamental genetic difference, diseases caused by genes located on the X chromosome affect males and females differently and thus present unusual inheritance patterns.Mutations in X linked genes will always affect males.If the mutation is recessive, females will generally be unaffected while in a dominant condition they will generally be affected.
3 X linked genes The X chromosome spans about 155 million base pairs It represents approximately 5 percent of the total DNA in cells.It is estimated to contain approximately 1400 genes (including non-coding RNA)About 308 "disease genes" have been found on the X chromosome
4 Conditions Related To Genes On The X Chromosome Allan-Herndon-Dudley SyndromeAlport SyndromeAmelogenesis ImperfectaAndrogenetic AlopeciaAndrogen Insensitivity SyndromeAarskog-Scott syndromeCharcot-Marie-Tooth DiseaseChoroideremiaCoffin-Lowry SyndromeColour Vision DeficiencyCongenital Hemidysplasia With Ichthyosiform Erythroderma And Limb Defects (CHILD)Cornelia De Lange SyndromeDuchenne And Becker Muscular DystrophyEmery-Dreifuss Muscular DystrophyFabry DiseaseFragile X SyndromeFrontometaphyseal DysplasiaGlucose-6-Phosphate Dehydrogenase DeficiencyHaemophiliaHypohidrotic Ectodermal DysplasiaIncontinentia PigmentiKallmann SyndromeL1 SyndromeLenz Microphthalmia SyndromeLesch-Nyhan SyndromeLowe SyndromeMcleod Neuroacanthocytosis SyndromeMelnick-Needles SyndromeMenkes Syndrome3-Methylglutaconic AciduriaNonsyndromic DeafnessNorrie DiseaseOcular AlbinismOculofaciocardiodental SyndromeOpitz G/BBB SyndromeOrnithine Transcarbamylase DeficiencyOtopalatodigital Syndrome Type 1Otopalatodigital Syndrome Type 2Paroxysmal Nocturnal HemoglobinuriaPelizaeus-Merzbacher DiseasePeriventricular HeterotopiaRett SyndromeSimpson-Golabi-Behmel SyndromeSpastic Paraplegia Type 2Spinal And Bulbar Muscular AtrophyTurner SyndromeX-Linked Adrenal Hypoplasia CongenitaX-Linked AdrenoleukodystrophyX-Linked AgammaglobulinemiaX-Linked Juvenile RetinoschisisX-Linked LissencephalyX-Linked Severe Combined ImmunodeficiencyX-Linked Sideroblastic AnemiaX-Linked Spondyloepiphyseal Dysplasia Tarda
5 X Chromosome Inactivation Restores equal dosage of expression from genes on the X chromosome between males and females.Early in embryonic development in females, one of the two X chromosomes is randomly and permanently inactivated in somatic cells.Females are mosaics for two cell populations, one with the maternal and one with the paternal X as the active chromosome.In a female heterozygous for an X linked condition -each cell will express either the normal or abnormal allele but not both.X inactivation “blurs” the distinction between dominant and recessive X linked conditions.
6 Skewed X Chromosome Inactivation X-linked disorders are generally rare in females and are usually attributable to advantageous silencing of the X chromosome that carries the mutant allele.Initial X-inactivation choice is presumed to be random.During proliferation, cells that have chosen the mutant X chromosome to be active can have a significant or total selective growth disadvantage - underrepresented in the adult carrier (negative cell selection).Postinactivation cell selection has been documented in carriers for a number of X-linked diseases e.g. Aarskog-Scott syndrome, X linked SCIDIn contrast, the positive growth advantage of cells in which the mutant X is primarily active has been reported in a few conditions.
7 Female Carriers of X linked conditions In general the boundaries between X linked dominant and recessive diseases might not be well defined for a number of cases displaying intermediate disease penetrance in heterozygous females.When the phenotype depends on a circulating product e.g. in haemophilia, there is an averaging effect between the normal and the abnormal cells.And so female carriers may have an intermediate phenotype and are usually clinically unaffected but biochemically abnormalIncreased CK levels and muscle weakness in carriers of DMD.Many “carrier” females can display classical disease symptoms of Fabry disease.
9 X linked recessive inheritance The vast majority of affected individuals are male (no back-up copy).No male-to-male transmission.All daughters of affected males are obligate carriers.Female carriers pass the defective X chromosome to half their sons (affected) and half their daughters (carriers).All affected males in a family are related through their mothers.Trait or disease is typically passed from an affected grandfather, through his carrier daughters, to half of his grandsons.The trait can “skip” generations (only carrier females and unaffected males are born).e.g. Duchenne/Becker Muscular Dystrophy, Alport, Haemophilia A and B, Menkes disease, Norrie disease
10 Manifesting CarriersReasons that a female may be affected with an X-linked recessive disorderThe affected female is homozygous for the mutant allele (has an affected father and carrier mother).Cells with the normal X inactivated could be present in disproportionate numbers (skewed X inactivation).The affected female has a 45,X karyotype or have an X chromosome with deletion of a normal gene.Genetic defect in the X-inactivation process itself (reported female affected with Wiskott-Aldrich syndrome).Caused by mutations in autosomal genes (genocopies) that have the same clinical effect as a mutation in an X-chromosome gene in males.
12 X-linked dominant Inheritance Dominant X-linked mutations are rare.Affects either sex, but more females than malesInheritance of mutant X – will get the diseaseFemales are more mildly and more variably affected than malesThe child of an affected female regardless of its sex has a 50% chance of being affectedFor an affected male, all of his daughters but none of his sons will be affected.Examples include Chondrodysplasia Punctata, Rett Syndrome, Incontinentia Pigmenti, X-linked hypophosphatemia, CHILD.
13 X linked dominant inheritance with male lethality Affected males are rarely or never seen because of a lethal effect of the mutant allele in the hemizygous male.Only females (and boys with Klinefelter Syndrome) are affected The pedigree may appear to have several spontaneous abortions in the offspring of affected females.As a result the sex ratio of their live offspring is often skewed.Examples include Incontinentia Pigmenti and Aicardi Syndrome
14 X linked dominant with male sparing Epilepsy and mental retardation limited to females (EFMR) is a disorder with an X-linked mode of inheritance and an unusual expression pattern.Disorders arising from mutations on the X chromosome are typically characterized by affected males and unaffected carrier females.In contrast, EFMR spares transmitting males and affects only carrier females.All daughters of males are affected, whereas none of their sons are affected.Another example of a condition with this unusual mode of inheritance is Craniofrontonasal syndrome (CFNS).
15 Possible explanations for this paradoxical phenotypic pattern In contrast to most other known X-linked recessive or dominant disorders, the milder phenotype of the males is unexplained. Possible explanations:‘Metabolic interference’ - the interaction of a normal and a mutant allele in a multimeric protein produces more severe dysfunction than the mutant allele aloneA functional homologue on the Y chromosome may ameliorate the effect of the X chromosome mutation.A disturbance in the process of X inactivation of the mutant gene could create a condition of functional disomy in females (Males would be unaffected by this).It might be a sex-limited disorder, in which the greater severity in females is explained by different interaction of the mutant gene with sex-specific developmental pathways.
16 Pseudoautosomal region. Some genes on the X chromosome escape X-inactivation.These genes are located at the tip of the short (p) arm of the X chromosome - the pseudoautosomal region.Genes in the pseudoautosomal region are present on both chromosomes - men and women each have two functional copies of these genes.Many genes in the pseudoautosomal region are essential for normal development.one gene called SHOX -important for bone development and growth.Short stature homeobox (SHOX)-related haploinsufficiency disordersInherited in a pseudoautosomal dominant manner
17 Complications to X linked inheritance patterns Skewed X inactivation (affected females e.g Fabry)High rate of new mutations (DMD)Germinal Mosaicism (DMD)Non-penetrance (FraX described as X-linked dominant with incomplete penetrance)Anticipation (Sherman paradox)Variable Expression (Lesch Nyhan)Consanquinity (affected females and apparent male-to male inheritance)
18 ReferencesHuman Molecular Genetics 3 (Strachan and Read)Dobyns WB The pattern of inheritance of X-linked traits is not dominant or recessive, just X-linked, Acta Pædiatrica, 2006; Suppl 451: 11_/15Dibbens LM et al., X-linked protocadherin 19 mutations cause female-limited epilepsy and cognitive impairment. Nat Genet Jun;40(6):Jakub Minkset al., A skewed view of X chromosome inactivation Clin. Invest. 118:20–23 (2008).Morleo and Franco. Dosage compensation of the mammalian X of X-linked dominant male-lethal disorders chromosome influences the phenotypic variability J. Med. Genet. 2008;45; ;Parolini 0, Ressmann G, Haas OA, et at. X-linked Wiskott-Aldrich syndrome in a girl. N Engl J Med 1998;338:291-5.Plenge RM et al., Skewed X-Chromosome Inactivation Is a Common Feature of X-Linked Mental Retardation Disorders. Am J Hum Genet Sep;79(3):493-9.Puck and Willard. Editorial: X inactivation in females with X-linked disease NEJM(5) 325-Am J Hum Genet July; 71(1): 168–173.Hladnik U et al., Variable Expression of HPRT Deficiency in 5 Members of a Family With the Same Mutation Arch Neurol. 2008;65(9):