Presentation on theme: "Unit 1 Cell and Molecular Biology"— Presentation transcript:
1Unit 1 Cell and Molecular Biology Section 4Molecules
2Structure and function of cell components CarbohydratesLipidsProteinsNucleic Acids
3CarbohydratesCarbohydrates are chemical structures containing C, H, and O in a ratio of 1:2:1The general formula is(CH2O)n
4Facts about monosaccharides Monosaccharides are molecules with the general formula (CH2O)n.The main example is glucose.Monosaccharides such as glucose are allof low molecular weightsweetsolublecrystalline.Monosaccharides such as glucose are used as sources of energy.
5Glucose chain structure Glucose is an example of an aldose sugar as its terminal group C1 is an aldehyde (CHO).Glucose is a reducing sugar due to the presence of the carbonyl group CO which can donate electrons.It is possible for the atoms in a 6-carbon sugar to take up different positions on the carbon chain. This leads to structural isomers
6Optical isomers of glucose Since molecules are 3-dimensional in shape optical isomers can be formed which are structurally identical but are mirror images of each other.D-glucose with OH on right of C6.L-glucose with OH on left of C6.
7Ring structure of Glucose Since glucose is a relatively long molecule, groups within it can react and change the shape of the molecule to form a pyranose ring structure.b-Da-D
8Glucose Reacting to form disaccharides The monomer glucose reacts by condensation (or dehydration) to form disaccharides.Water is removed in the process.
9Maltose is linked by an a (1-4) glycosidic bond. In the example below two molecules of a-D glucose react together to form maltose.The bond holding the glucose molecules together (highlighted in red) is known as a glycosidic bond.Maltose is linked by an a (1-4) glycosidic bond.glycosidic bondIf two β-D glucose join the result is cellobiose and the bond is at an angle (top to bottom)
10Structure and function of polysaccharides Polysaccharides are complex carbohydrates made up linked monosaccharide units.When a polysaccharide is made up of one type of monosaccharide unit it is called a homopolysaccharide.Starch and glycogen are polysaccharides used for energy storage.Other polysaccharides such as cellulose and chitin may be structural in function.
11StarchStarch is a storage compound in plants, being insoluble in water.It is a homopolysaccharide made up of two components: amylose and amylopectin.
12Amylose – a straight chain structure formed by 1,4 glycosidic bonds between a-D-glucose molecules. HOCH2OHOHC4Structure of Amylose Fraction of Starch
13The amylose chain forms a helix. This causes the blue/black colour change on reaction with iodine.
14The structure of the Amylopectin Fraction of Starch Amylopectin is a branched structure due to the formation of 1,6 glycosidic bonds.COC1HHOC6H2OHOHC4CH2OHThe cross linkages are formed by dehydration reactions between carbon 1 of one chain and carbon 6 of a parallel chainStart of chain 2End of chain 1
15Amylopectin causes a red-violet colour change on reaction with iodine. This change is usually masked by the much darker reaction of amylose to iodine.Starch therefore consists of amylose helices entangled on branches of amylopectin.Shows branching of amylopectin
16GlycogenGlycogen is a homopolysaccharide made from repeating a-D-glucose units and is very similar in structure to amylopectin, i.e. it has a highly branched structure.Glycogen is a storage compound in animals; including humans.It causes a red-violet colour on addition of iodine (similar to amylopectin).
17Cellulose Cellulose is the most abundant organic material on earth. Most animals however lack the enzyme cellulase required to break it down to its component monomers.Cellulose is made up of long straight chains of b-glucose molecules.
18The b-glucose molecules are joined by condensation, i. e The b-glucose molecules are joined by condensation, i.e. the removal of water, forming b(1,4) glycosidic linkages.Note however that every second b-glucose molecule has to flip over to allow the bond to form. This produces a “heads-tails-heads” sequence.
19The glucose units are linked into straight chains each 100-1000 units long. Weak hydrogen bonds form between parallel chains binding them into cellulose microfibrils.Cellulose microfibrils arrange themselves into thicker bundles called macrofibrils. (These are usually referred to as fibres.)The cellulose fibres are often “glued” together by other compounds such as hemicelluloses and calcium pectate to form complex structures such as plant cell walls.
20Other Polysaccharides Chitin is the main structural component of the exoskeleton of arthropods (e.g. spiders, insects and crustaceans) and the walls of fungi such as yeast.Chitin is structurally similar to cellulose but the monomer is an amino sugar called glucosamine.Glucosaminoglycans are complex heteropolysaccharides found in the connective tisues and skin of vertebrates.
21ActivityRead Dart Pg 25-31Scholar 4.2 carbohydratesPractice drawing different molecular structures
22LipidsLipids have a varied structure but all have the following properties in common:-Insoluble in waterSoluble in organic solventsThe three main groups of lipids are:-TriglyceridesPhospholipidsSteroidsLipids are important in cell membrane structure and also as energy storage molecules and hormones.
23Structure of glycerolGlycerol is a three carbon alcohol that contains 3 –OH (hydroxyl) groups
24Structure of Fatty Acids Fatty acids are hydrocarbon chains ending in a carboxyl group (COOH)R is an abbreviation for any organic groupAbout 30 different fatty acids are commonly found in lipids (they nearly always have an even number of carbon atoms).HO – C – RO
25Saturated fatty acids All available bonds are occupied by hydrogens E.gPalmitic acid CH3(CH2)14COOHStearic acid CH3(CH2)16COOHOH – C – C – C – C – C – C – C – C – C – C – C – C – C – C – C – CH3O
26Unsaturated fatty acids Some carbon atoms are double bonded with one another, therefore they are not fully saturated with hydrogenE.g. Oleic Acid CH3(CH2)7 CH = (CH2)7COOHNote - this is monounsaturated (1 double bond)
27E.g. Linoleic acid CH3(CH2CH=CH)3(CH2)7COOH Note – this is polyunsaturated (more than 1 double bond)
28Formation of Ester Linkages Glycerol and fatty acids are joined together by dehydration (condensation) reactionsThe bond linking glycerol and fatty acids is called an ester bond
31TriglyceridesTriglycerides consist of a single glycerol molecule and three fatty acids.Glycerol
32Glycerol (blue) is an alcohol derivative of glyceraldehyde and has three hydroxyl groups. It acts as the backbone of the structure.Fatty acids (red) – there are more than 70 types of fatty acid but they all have long hydrocarbon tails and a terminal carboxyl group (COOH). The variety of fatty acids determine the properties of each triglyceride.
33Formation of Triglycerides Triglycerides form by condensation (dehydration) reactions between the hydroxyl (OH) groups of the glycerol and the carboxyl (COOH) group of three fatty acids.Triglycerides are esters being derived from an alcohol and a fat.
35Triglycerides in plants Plants store their energy in triglycerides with low melting points which are liquid at room temperature.These triglyceridesare referred to as oilsresult from reaction between glycerol and an unsaturated fatty acid e.g. oleic acid.
36Triglycerides in Animals Animals store their energy in triglycerides with high melting points which are solid at room temperature.These triglyceridesare referred to as fats.result from reaction between glycerol and a saturated fatty acid e.g. stearic acid.
37Triglycerides in cells Triglycerides are insoluble in water because they have no charge i.e. they have covalent bonds. This causes them to form droplets in the cytoplasm
38Functions of triglycerides Energy storage - triglycerides contain twice the energy/gram of carbohydrates or proteins. During aerobic respiration triglyceride is broken into 2C portions which are fed into the Krebs cycle.Source of metabolic water. water is released on the breakdown of triglycerides and this property is used efficiently is by desert mammals.
39Insulation – triglycerides are found in the blubber of whales and other aquatic animals. Buoyancy – aquatic animals use triglycerides to help them float as they are less dense than water.
40PhospholipidsThe structure of phospholipids is based on the structure of triglycerides but the third hydroxyl group of the glycerol is linked to phosphoric acid which is often linked to a large polar group.
41The fatty acids which make up phospholipids have a consistent length of between 16 and 18 carbons. This allows them to form neat bilayers.Phospholipids are said to be amphipathic, having two very different sides to their nature.
42Hydrophilic portionHydrophobic portionThe ‘head’ containing the polar group and the phosphate group has polar covalent bonds. It is slightly charged and attracts water, i.e. it is hydrophilic.The ‘tail’ containing the long hydrocarbon group which is non-polar covalent. It is not charged and repels water, i.e. it is hydrophobic.
43The amphipathic nature of phospholipids is important in the formation of bilayers such as cell membranes.The hydrophilic groups line up on the outside faces of the membrane. The hydrophobic portions are arranged within the membrane.
44Phospholipids may have fatty acids which are saturated or unsaturated Phospholipids may have fatty acids which are saturated or unsaturated. This affects the properties of the resulting bilayer/cell membrane:Most membranes have phospholipids derived from unsaturated fatty acids.Unsaturated fatty acids add fluidity to a bilayer since ‘kinked’ tails do not pack tightly together.Phospholipids derived from unsaturated phospholipids allow faster transport of substances across the bilayer.
45Membranes exposed to the cold have a very high percentage of unsaturates e.g. bacteria grown at low temperature or the membranes of reindeer ears – remember unsaturates are liquid at much lower temperatures.
46Membranes which are stiffer such as those in nerve cells contain a much higher percentage of phospholipids derived from saturated fatty acids.They also contain high levels of cholesterol which stiffens membrane structure further.
47Steroids Steroids have a common four ring structure. Each unit within the four-ring structure is known as an isoprene unit (C5H8).
48Different steroids vary in the side chains attached to the rings. Notice that cholesterol and testosterone are almost identical except for the side groups on C3 and C17.
49Steroids are classified as lipids since they are soluble in organic compounds but not in water. They have a very powerful effect because of this as they can pass through cell membranes.Steroids are hormonal in function and have a wide variety of functions.Other examples of steroids are oestrogen, progesterone, cortisol, cholesterol and aldosterone.
50ActivityRead and take notes from Dart Pg 32-37Scholar section 4.3Use the internet to familiarise yourself with different ways of presenting the chemical formulae / structures
51Amino acidsAmino acids are the structural building blocks (monomers) of proteins.There are twenty different kinds of amino acids used in proteins.Proteins are referred to as heteropolymers due the variety of amino acids involved in their structure.
53Amino acids, like carbohydrates, show isomerism Amino acids, like carbohydrates, show isomerism. Proteins are only made up of amino acids which are L-isomers.L-isomerD-isomer
54At neutral pH’s amino acids exist in an ionised form and have both acidic and basic properties. This is because the carboxylic group donates hydrogen ions to the solution (acidic) whereas the amino group (NH2) attracts hydrogen ions from the solution.The repeating sequence of atoms along a proteins is referred to as the polypeptide backbone. Attached to this repetitive chain are the different amino acid side chains (R-groups) which are not involved in the peptide bond but which give each amino acid its unique property.
55acidic basic uncharged polar non polar Amino acids are grouped according to whether their side chains are:-acidicbasicuncharged polarnon polar
59Non-polar amino acids Isoleucine ile Neutral Non-polar Methionine met
60The type of side chain is very important as it affects the solubility of the amino acid. Hydrophobic features include long non-polar (uncharged) chains or complex aromatic rings.Hydrophilic features include additional carboxyl groups or amino groups not involved in peptide bonding which are ionised in solution.
62Structure of proteins Primary structure Secondary structure The sequence of amino acids in a given protein is known as its primary structure.Secondary structureSimple proteins with regularly repeating amino acids often form a secondary structure due to hydrogen bonds between the amino group ( NH) and carbonyl group ( CO ) of adjacent amino acids.This additional bonding may twist the long protein chain into a helix known as an alpha helicesThis secondary bonding gives rise to proteins which are structural e.g.Collagen – 3 alpha helices twisted togetherElastinKeratin -7 alpha helices twisted together
64A second formation resulting from hydrogen bonds between adjacent peptide bonds is known as β- pleated sheetsAn example of a protein made up of β- pleated sheets is fibroin found in spiders webs which is extremely strong.
65Tertiary structureThe third type of structure found in proteins is called tertiary protein structure.The tertiary structure is the final specific shape that a protein assumes.This final shape is determined by a variety of bonding interactions between the "side chains" on the amino acids. These bonding interactions may be stronger than the hydrogen bonds between amide groups holding the helical structure.Bonding interactions between "side chains" may cause a number of folds, bends, and loops in the protein chain. Different fragments of the same chain may become bonded together.There are four types of bonding interactions between "side chains" including:hydrogen bonding,salt bridges,disulfide bonds,non-polar hydrophobic interactions.Globular proteins such as enzymes, antibodies, and cell membrane proteins all show tertiary structure
69The hydrophobic interactions of non-polar side chains are believed to contribute significantly to the stabilizing of the tertiary structures in proteins.Non groups such as benzene rings repel water and other polar groups and results in a net attraction of the non-polar groups for each other
70Quaternary StructureThe quaternary protein structure involves the clustering of several individual peptide or protein chains into a final specific shape.A variety of bonding interactions including hydrogen bonding, salt bridges, and disulfide bonds hold the various chains into a particular geometry.There are two major categories of proteins with quaternary structure - fibrous and globular.Fibrous Proteins:Fibrous proteins such as the keratins in wool and hair are composed of coiled alpha helical protein chains with other various coils analogous to those found in a rope. Other keratins are found in skin, fur, hair, wool, claws, nails, hooves, horns, scales, beaks, feathers, actin and mysin in muscle tissues and fibrinogen needed for blood clots.
72Globular ProteinsOn the other hand, globular proteins may have a combination of various individual units of various shapes which are mostly clumped into a shape of a ball. Major examples include insulin, hemoglobin, and most enzymes.
73Nucleotide structures The building block of a nucleic acid is a nucleotide.Nucleotides consist ofA pentose sugarA nitrogenous baseA phosphate group
74Pentose SugarsDeoxyribose and ribose differ by the group attachment at the 2’C.
75Bases Purines Pyrimidines There are 5 bases These can be classified into two typesPurinesDouble ringedAdenine and GuaninePyrimidinesSingle ringedCytosine, Thymine and Uracil
76Formation of a nucleotide A condensation reaction occurs between the OH group on the 5’ C and phosphate group to form a strong phosphodiester bond.A condensation reaction occurs between the base and the 1’ C to form a strong glycosidic bond
81Enzymes DNA polymerase RNA polymerase Catalyses the linking together of DNA nucleotides during replicationDNA polymerase can only add a nucleotide to the 3’ end of the previous nucleotide.RNA polymeraseCatalyses the linking of RNA nucleotides during transcription (and in the replication of the lagging strand during DNA replication)
82DNA ligase Joins short sections of DNA together DNA replication animation:
83Activity Read DART pg 48 – 53 and take notes Scholar 4.5 Look atReplication forkDNA replicationMeselson-Stahl experimentMake notes on DNA and RNA structureMake notes on replication and transcriptionThink about how you will remember which bases are purines and which are pyrimidinesWrite a summary of all the different bonds formed between molecules by dehydration reactions.