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SPERM HYLITER Introduction to SPERM HY-LITER TM Tests for Forensic Laboratories Monoclonal Antibody-Based Fluorescent Detection of Human Sperm from Sexual.

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Presentation on theme: "SPERM HYLITER Introduction to SPERM HY-LITER TM Tests for Forensic Laboratories Monoclonal Antibody-Based Fluorescent Detection of Human Sperm from Sexual."— Presentation transcript:

1 SPERM HYLITER Introduction to SPERM HY-LITER TM Tests for Forensic Laboratories Monoclonal Antibody-Based Fluorescent Detection of Human Sperm from Sexual Assault Evidence SPERM HY-LITER TM SPERM HY-LITER PLUS TM

2 SPERM HYLITER SPERM HY-LITER TM SPERM HY LITER - Test Format: Microscopic Screening Method for Sperm Antigen Detected: Human Sperm Heads Antibodies: Mouse monoclonal Labeling:Sperm: Alexa 488 / Nuclei: DAPI Sensitivity:Single Sperm Head Cross-reaction:None detected Test Read-out:Visual Preparation Time:120 minutes – 10 minutes ‘hands-on’

3 SPERM HYLITER SPERM HY-LITER TM SPERM HY LITER - SPERM HY-LITER TM staining Sensitivity & Specificity Buccal, Urine & Blood Extracts Down to a Single Sperm Phase Contrast DAPI FilterFITC Filter

4 SPERM HYLITER SPERM HY LITER - SPERM HY-LITER TM staining Species Specificity Animal Semen (various) without Human Semen Phase Contrast DAPI FilterFITC Filter SPERM HY-LITER PLUS TM

5 SPERM HYLITER SPERM HY-LITER TM SPERM HY LITER - SPERM HY-LITER TM staining Species Specificity Animal Semen (various) with Human Semen Phase Contrast DAPI Filter FITC Filter

6 SPERM HYLITER SPERM HY-LITER TM SPERM HY LITER - First Positive Identification for Human Sperm First Specific Identification for Human Sperm Sensitivity down to a single sperm Fully Compatible with Automated Methods SPERM HY-LITER PLUS TM Scan slides at lower magnification: 40X

7 SPERM HYLITER Jennifer Old, Ph.D. Brett Schweers, Ph.D. P.W. Boonlayangoor, Ph.D. Chicago Liz Graffy, MS Marisa Farhner, M.S Acknowledgements : SPERM HY-LITER PLUS TM SPERM HY-LITER TM

8 SPERM HYLITER Questions CommentsSamplesGripes Karl Reich Tel 708.234.1200 Fax 708.978.5115 karl@IFI-test.com


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