Presentation on theme: "Enzymatic Determination of Creatine Kinase (CK) Activity"— Presentation transcript:
1 Enzymatic Determination of Creatine Kinase (CK) Activity
2 So… what does that mean?CK is an enzyme that everyone has in their bodies.It is mainly located in the heart & skeletal muscles.Damage to these causes CK to be released into the bloodstream.Therefore, if you test the blood & find a heightened level of CK, it can be determined that the body has received damage.
3 However…There is more than one type of CK; therefore, more tests are required.CK2 comes from the heart while CK3 comes from the muscles.-More on that from Justin & Kendra.
4 How it’s doneFirst, you take the CK Reagent (2.4ml; this contains everything needed to react with the CK in the samples).Then, you add your sample (0.1ml) & mix with the vortex-genie.Next, you put it into the prepared spectrophotometer & read the absorbance reading provided by the machine at given times.
5 What is a spectrophotometer, & why did we use one? A spectrophotometer is a device that shines light into a sample & measures how much light was absorbed by the sample.We used one because the products of the CK & CK Reagent reaction are light absorbent.Therefore, the samples with more CK will have more products which will absorb more light.
6 What we learned…How to properly use lab equipment including spectrophotometers, pipettes, pipetters, capillaries, & etc.What CK is & how it relates to our heartHow to measure CKHow not to get CK Reagent on ourselves