H075D08 H044O16 H075D08 H075D08- H044O16 -confirm (397bp) H044O16 confirm (427bp) GGCCGTTCAACTTGCTCTTA LEFT PRIMER GGCACAAACATGTCAAATGC RIGHT PRIMER GGCCGTTCAACTTGCTCTTA LEFT PRIMER CTGAATTCGCGAACCAATCT RIGHT PRIMER
H125L12 (12,174bp) 1bp E011K05 (91,579bp) 671bp6,462bp 5,798bp H125L12 (12,174bp) T0493 (48.0 cM) Expect 11,466bp E011K05 (91,579bp) H125L12 E011K05 Sequence result Dot-Matrix result Two sequence alignment result 2_G Case 5
Summary NameGenomeEnzyme siteNameGenomeEnzyme site Mbo108P14XOHBa075D08XX Mbo008E03XOMbo049G16OX HBa323A14OXHBa044O16XX HBa059M17OX
10 20 30 40 50 60 70 80 90 100 110 140 120 130 0 142 13 Probably, chimeric BAC clones Were selected as “Next BAC “ clones during extension process 1.Chimeric BAC clone itself exist in libraries but not in genome. 2. Not all strange BAC clones have enzyme site.
What is best way to speed up sequencing progress? Increase seed BAC 64 Seed BACs 74 Extended BACs 28 contigs 16 single BAC + increase extending chance - Increase overlap length
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