8Muscle endurance / Muscle recovery Improves Muscle EnduranceAstaxanthin protects muscle at the cellular level from damage of reactive oxygen species (ROS) generated by physical activity. Astaxanthin improves blood flow and modulates vasorelaxation, protecting the endothelium dependent NO effect and vessel elastin band structure from oxidative damageAccelerates Body Fat ReductionAstaxanthin enhances fat metabolism by activating mitochondrial enzymes such as 3-hydroxyacyl CoA dehydrogenase (3-HAD)Astaxanthin protects carnitine palmitoyl transferase I (CPT I) from oxidative modification caused by ROSReduces Lactic Acid Accumulation-Astaxanthin promotes fatty acid utilization vs glucose metabolism for Mitochondrial ATP production-Astaxanthin reduces peroxidation damage during anaerobic exercise resulting in muscle recovery time improvement
9Eye Fatigue results Our 10 Clinical Studies Demonstrate: • Increased Retinal Blood Flow• Improved Accommodation• Reduced Asthenopia• Increased Visual Acuity• Ocular Inflammation ReductionVDT Subjects with Eye Strain Symptoms before and after astaxanthin supplementation (Nagaki et al.,2002)
10AstaREAL® astaxanthin patents No. 6,245,818Muscle and Physical Endurance - increased physical endurance / reduced muscle damageNo. 6,262,316Gastric Health - Reduces infection/inflammation of Helicobacter pyloriNo. 6,335,015Mastitis - Prophylactic treatment for mastitisNo. 6,410,602Fertility - Method of increasing the production and improving the quality of semenNo. 6,773,708Immune System - Immunomodulation - autoimmune diseases and chronic viral and intracellular bacterial infectionsNo. 6,923,967Dyspeptic Symptoms - Alleviates dyspeptic symptomsNo. 7,078,040Anti-Inflammation - Cytokine regulation - inhibiting the expression of inflammatory cytokines and chemokinesPatent PendingEye Fatigue - Reduced eye fatigue / improved visual acuityCardiovascular – Alleviating vascular failureDiabetic Nephropathy - Gene expression modulator –suppression of development of diabetic nephropathy
11Member of the Carotenoid Family 732 known carotenoids*Humans cannot synthesise carotenoids.Need to obtain carotenoids from food.Basic carotene structureXanthophyll Class- Lutein- Astaxanthinisolated and identified fromlobster Astacus gammarusin 1938 by Kuhn-CaroteneOHLuteinAstaxanthinMolecular Formula: C40H52O4Molecular Weight:CAS No:*G. Britton et al., Carotenoids Handbook, 2004
12Astaxanthin is a Totally Unique Antioxidant Powerful & Safe Antioxidant- Singlet oxygen quenchingLipid peroxidation suppressingWithout any pro-oxidative natureSuperior Position in Cell Membrane- Protection both hydrophillic & lipophillic area
13Astaxanthin is the Most Powerful Quencher of Singlet Oxygen in Nature 36 Times Stronger than Beta-Carotene (1)75 Stronger than a-Lipoic Acid (2)550 Times Stronger than Vitamin E (1)560 Times Stronger than Green Tea (2)794 Times Stronger than CoQ10 (2)6000 Times Stronger than Vitamin C (2)Astaxanthin is the Most Powerful Quencher of Singlet Oxygen in Nature1. Shimizu et al., Carotenoids as singlet oxygen quenchers in marine organisms, Fisheries Science, 62, (1996).2. Nishida et al., Quenching Activities of Common Hydrophilic and Lipophilic Antioxidants against Singlet Oxygen Using Chemiluminescence Detection System, Carotenoid Science.,Vol.11: (2007)13
14Why is Astaxanthin Superior? Astaxanthin does not Become Pro-Oxidant Even under Enormous Oxidative StressH.D.Martin, et al. : Chemistry of carotenoid oxidation and free radical reactions, Pure Appl. Chem., 71(12), (1999).
15Superior Position in Membrane Beta-caroteneVitamin C
17Production Haematococcus Pluvialis Haemato (Blood) Coccus (Seed) Pluvialis (Fresh Water)Vegetative cell Immature Aplanospore Aplanospore４０－８０μｍProductionHaematococcus pluvialis cultivationMicroalgae family – includes Chlorella and SpirulinaSingle cell fresh water Microalgae (Plant source)Found in standing pools and ponds world wide (non-GMO)During harsh environment conditions – Hibernates
18Open Pond System Closed System Mass Production of Haematococcus pluvialisOpen Pond System- Outdoor, susceptible to environmental contaminants- Difficult to control unwanted foreign algae- Inconsistent production and yieldClosed System- AstaREAL Indoor closed tank systemconsistently has the highest yield withthe longest history of productionConc.→ MatureChlorophyllAstaxanthin
19Closed Tank SystemMost cost effective cultivationTotal environmental controlHighest quality, cleanest systemFirst in the world to commercially cultivate Haematococcus pluvialis
2030 Safety Studies. Submitted for GRAS. Health and SafetyFull Acute & Subchronic TestsNO ADVERSE SIDE EFFECTS REPORTEDAmes Test & Gene ToxicityWorldwide Literature Search30 Safety Studies. Submitted for GRAS.1/6/2010 received FDA non-objection letter.