Presentation on theme: "The 6420 Triple Quad LC/MS System"— Presentation transcript:
1 The 6420 Triple Quad LC/MS System Routine, Robust and Reliable Quantitation1
2 Agilent has the Newest Fleet of LC Triple Quads A New Agilent LC Triple QuadLaunchedEvery YrFor last4 yrs6460TripleQuad6430 Triple Quad6420 Triple Quad64906460 Triple QuadPerformance2
3 6420 Enhancements over Earlier 6410 New Capillary front end: faster polarity switching better ion transmissionNew electronics, faster MRMsNew Agilent rough pumpUpgradeable to a 6460 with Agilent Jet Stream Technology6420 Triple Quad LC/MSWaters requires a 10 msec interscan delay to achieve zero cross-talk.This argument has been successfully used in a few sales situations.
4 6420: Fast Electronics for High Sample Throughput The Agilent 6420 Triple Quad features fast and rugged electronics;Making it a workhorse for high throughput labsFast MRMs , dwell time of: 1 msecFast polarity switching : 30 msecFast Triggered MRM(for ten ion transitions): msec
6 6420 Upgrade Possibilities S/N SpecProductUpgradeOptionsInstalledin yourLab6430to 6460AgilentJet Stream& High Mass QuadsYes6420to 6430SecondTurbo8X6460TripleQuad6430 Triple Quad2X6420 Triple Quad1X
7 New 6420 LC Triple Quad Supports: Agilent Upgrade Kits to 6430 and 6460 LC Triple QuadsAgilent Dynamic MRMAgilent Fast Track Kits and Data BasesAgilent Triggered MRM
8 Upgradeable by Design – 6400 Upgrades 6420 to 6430 UpgradeImproves Signal-to-Noise up to two-fold for some compoundsThe 6420 is preconfigured to accept a second turbo pump for enhanced sensitivity.Higher vacuum in Q3 reduces noise and allows greater ion conductance in the collision cell.
9 6430 to 6460 Add: Agilent Jet Stream Technology thermal confinement of ESI spray cone 6430 to 6460 UpgradeSensitivity Spec: 4X higher25 0C350 0C after 8 min
10 6430 to 6460 Upgrade: High Mass Quads – 3K The 6460 mass range is 3,000 m/z:Pegylated peptidesAcylated peptidesLipopolysaccharidesGlycansNatural Products
11 Dynamic MRM groups MRMs in Retention Windows Dynamic MRM creates powerful quantitative methods by grouping MRMs in retention time windows instead of time segments.Dynamic MRM methods are created by importing compound specific MRMs and retention times from a Fast Track Data Base or worksheetDynamic MRM supports up to 4,000 MRMS for all 6400 Series Triple QuadsDynamic MRM also supports polarity switching
12 Dynamic MRM for the new 6420 Triple Quad 12345678910111213141516Retention Time Windows with overlapping MRMs
13 Agilent Fast Track Kit for Forensics Includes a Dynamic MRM method,200 compound data base withoptimal settings and RTsFORENSIC/TOXICOLOGY TEST MIX – 24 compoundsCompound Name Precursor Ion Product Ion Collision Energy Ret TimeHydrocodone methamphetamineMDMAStrychnineMDEA……
14 Forensics Test Mixture on the new 6420 25 pg of each on-columnAcquisition Time (min)18.104.22.168.822.214.171.124.8126.96.36.199.844.2AmphetamineMetham-phetamineTrazodonePCPNorver-apamilVerapamilMethadoneProadifenBenzoyl-ecgonineCocaine
15 Triggered MRM (tMRM) – a Data Dependent Scan Data dependent execution of additional, confirmatory MRMs152.177.0105.0219.0m/z60100140180220209.1165.1triggered MRM Spectra2-Ethylanthraquinone
17 tMRM Product Ion Spectrum on any 6400 Triple A Re-Constructed Product Ion Spectrum that is Library Searchable334 > 76334 > 91334 > 105334 > 117334 > 119x103x103x103x103x10387654321876543218765432187654321117.08765432176.091.1105.1119.080145.0334 > 171x103334 > 132x103334 > 145x103334 > 147x10387654321876543218765432187654321147.0132.1171.1tMRM Product Ion SpectrumTebufenpyradx103145.0117.087654321This is an illustration of how tMRM acquisition generates the product ion spectrum for each triggered analyte.Up to ten MRM transitions are acquired for each target (the primary transitions are typically the transitions with the highest product ion abundance).The tMRM software then compiles the primary and secondary MRM spectra into a single, library searchable, product ion spectrumWith up to ten product ions present in each tMRM product ion spectrum for each target analyte, there is sufficient data to confidently identify or confirm the analyte of interest with library confirmation. The biggest advantage of using tMRM to generate these product ion spectra (rather than conventional product ion scanning instruments), is that acquiring all ten of the MRM transitions which make up this spectrum takes about 20 ms, where a typical product ion scan on a trap or TOF instrument takes about 200 ms. The faster cycle time for qualitative analysis using tMRM acquisition means that more data points are acquired across each peak, and the quantitative data acquired is more sensitive, robust, and accurate.76.091.1105.1132.1171.1334.2
18 Benefits of triggered MRM More than ten times more sensitive than full scan MS/MS dataSearchable with NIST MS/MS 2.0 LibrarySearchable with Agilent Accurate Mass MS/MS LibraryCompatible with custom, user created librariesMassHunter automatically creates custom triggered MRM library from standards
19 Forensics Mixture with 6420 and tMRM Method Triggered MRM actuated on red peakAcquisition Time (min)188.8.131.52.8184.108.40.206.8220.127.116.11.844.2AmphetamineMetham-phetamineTrazodonePCPNorver-apamilVerapamilMethadoneProadifenBenzoyl-ecgonineCocaine
20 Triggered MRM for Compound Confirmation 6420 tMRM: Benzoylecgonine, 25 pg on columnMass-to-Charge (m/z)100150200250300-1.2-1-0.8-0.6-0.4-0.20.20.40.60.81168.177.0105.0290.1Ref. Library Match Score = 95.1Peak Product Ion SpectraLibrary ReferenceProduct IonSpectra@ 5ppb level:RSD=4.9% (N=3)
21 6420 SummaryDENVER (American Society for Mass Spectrometry conference), June 6, 2011The new Agilent 6420 Triple Quad LC/MS System is a workhorse triple quad designed to be readily upgradable to the higher-performance 6430 and 6460 instruments.The new 6420 is more productive than the previous 6410 model with faster electronics and new software features. Our popular dynamic MRM solution for grouping ion transitions by retention time has been enhanced with the addition of triggered MRM for simultaneous compound confirmation.The Agilent 6420 is well suited for both routine and complex analyses such as toxicology and drugs of abuse testing. It is designed for round-the-clock operation to support labs running a large number of samples.The 6420 replaces Agilent's popular Model 6410 triple quad. The 6420 maintains method and data compatibility with existing 6410 methods. The 6420 can be easily incorporated into high-productivity laboratories with multiple 6410 systems.
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