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1 13 Molecular Genetics of Cell Cycle and Cancer.

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1 1 13 Molecular Genetics of Cell Cycle and Cancer

2 2 There are two major parts in the cell cycle: Interphase: G 1 = gap1S = DNA synthesis G 2 = gap2 Mitosis: M There are two essential functions of the cell cycle: To ensure that each chromosomal DNA molecule is replicated only once per cycle To ensure that the identical replicas of each chromosome are distributed equally to the two daughter cells The Cell Cycle

3 3 Fig. 13.1

4 4 The Cell Cycle The cell cycle is under genetic control A fundamental feature of the cell cycle is that it is a true cycle: it is not reversible Many genes are transcribed during the cell cycle just before their products are needed Mutations affecting the cell cycle have helped to identified the key regulatory pathways

5 5 The Cell Cycle Progression from one phase to the next is propelled by characteristic protein complexes, which are composed of Cyclins and cyclin-dependent protein kinases (CDK) Expression of mitotic cyclins E, A, and B are periodic, whereas cyclin D is expressed throughout the cell cycle in response to mitosis stimulating drugs (mitogens) The cyclin-CDK complexes phosphorylate targeted proteins, changing dramatically their activity

6 6 Fig. 13.8

7 7 The Retinoblastoma Protein The retinoblastoma (RB) protein controls the initiation of DNA synthesis. RB maintains cells at a point in G1 called the G1 restriction point or start by binding to the transcription factor E2F, until the cell has attained proper size If the cycling cell is growing properly and becomes committed to DNA synthesis, several cyclin-CDK complexes inactivate RB by phosphorylation After cell enters S phase E2F becomes phosphorylated as well and loses its ability to bind DNA

8 8 Fig

9 9 The Cell Cycle The progression from G2 to M is controlled by a cyclin B-CDK2 complex know as maturation- promoting complex Protein degradation (proteolysis) also helps regulate the cell cycle. The anaphase-promoting complex (APC/C), which is a ubiquitin–protein ligase responsible for adding the 76-amino-acid protein ubiquitin to its target proteins and marking them for destruction in the proteasome Proteolysis eliminates proteins used in the preceding phase as well as proteins that would inhibit progression into the next

10 10 Checkpoints Cells monitor their external environments and internal state and functions Checkpoints in the cell cycle serve to maintain the correct order of steps as the cycle progresses; they do this by causing the cell cycle to pause while defects are corrected or repaired Checkpoints in the cell cycle allow damaged cells to repair themselves or to self-destruct

11 11 Three Main Checkpoints A DNA damage checkpoint A centrosome duplication checkpoint A spindle checkpoint

12 12 Fig

13 13 A DNA Damage Checkpoint A DNA damage checkpoint arrests the cell cycle when DNA is damaged or replication is not completed. In animal cells, a DNA damage checkpoint acts at three stages in the cell cycle: at the G1/S transition, in the S period and at the G2/M boundary The p53 transcription factor is a key player in the DNA damage checkpoint.

14 14 A DNA Damage Checkpoint In normal cells, level of activated p53 is very low Protein Mdm2 keeps p53 inactivated by preventing phosphorylation and acetylation of p53 and by exporting p53 from the nucleus Damaged DNA leads to activation of p53 and its release from Mdm2

15 15 A DNA Damage Checkpoint Activated p53 triggers transcription of a number of genes - p21, s, Bax, Apaf1, Maspin,GADD45 DNA damage detected in G1 blocks cell G1/S transition DNA damage in S phase reduces processivity of DNA polymerase and gives the cell time for repair Processivity: number of consecutive nucleotides that replicate before polymerase detaches from template DNA damage detected in S or G1 arrests cells at G2/M transition

16 16 Fig

17 17 A DNA Damage Checkpoint DNA damage also triggers activation of a pathway for apoptosis = programmed cell death When the apoptotic pathway is activated, a cascade of proteolysis is initiated that culminates in cell suicide The proteases involved are called caspases

18 18 Centrosome Duplication Checkpoint Monitors spindle formation Functions to maintain the normal complement of chromosomes Sometimes coordinates with the spindle checkpoint and the exit from mitosis

19 19 The Spindle Checkpoint Monitors assembly of the spindle and its attachment to kinetochores The kinetochore is the spindle-fiber attachment site on the chromosome Incorrect or unbalanced attachment to the spindle activates spindle checkpoint proteins, triggers a block in the separation of the sister chromatids by preventing activation of the anaphase-promoting complex (APC/C)

20 20 Fig

21 21 Cancer Cancer cells have a small number of mutations that prevent normal checkpoint function Cancer is not one disease but rather many diseases with similar cellular attributes All cancer cells show uncontrolled growth as a result of mutations in a relatively small number of genes Cancer is a disease of somatic cells

22 22 1% of cancer cases are familial: show evidence for segregation of a gene in pedigree 99% are sporadic: the result of genetic changes in somatic cells Within an organism, tumor cells are clonal, which means that they are descendants from a single ancestral cell that became cancerous. Cancer

23 23 Cancer Cells vs. Normal Cells In normal cells, cell-to-cell contact inhibits further growth and division, a process called contact inhibition Cancer cells have lost contact inhibition: they continue to grow and divide, and they even pile on top of one another

24 24 Cancer Cells vs. Normal Cells Even in the absence of damage, normal cells cease to divide in culture after about 50 doublings = cell senescence Senescence of normal cells is associated with a loss of telomerase activity: the telomeres are no longer elongated, which contributes to the onset of senescence and cell death Cancer cells have high levels of telomerase, which help to protect them from senescence, making them immortal

25 25 Key Mutational Targets Many cancers are the result of alterations in cell cycle control, particularly in control of the G1-to-S transition These alterations also affect apoptosis through their interactions with p53 The major mutational targets for the multistep cancer progression are of two types: Proto-oncogenes Tumor-suppressor genes

26 26 Key Mutational Targets The normal function of proto-oncogenes is to promote cell division or to prevent apoptosis The normal function of tumor-suppressor genes is to prevent cell division or to promote apoptosis

27 27 Oncogenes Oncogenes are derived from normal cellular genes called proto-oncogenes Oncogenes are gain-of-function mutations associated with cancer progression Oncogenes are gain-of-function mutations because they improperly enhance the expression of genes that promote cell proliferation or inhibit apoptosis

28 28 Mdm2: Amplification of Mdm2 gene and overexpression of Mdm2 protein leads to inactivation of p53 gene Amplification of Mdm2 gene has been found in many tumors of adipose tissue, soft tissue sarcomas, osteosarcoma, and esophageal carcinoma Cyclin D and CDK4: Amplification and overexpression leads to unscheduled entry to S phase Amplification and/or overexpression has been found in many esophageal carcinomas, bladder and breast cancers Oncogenes

29 29 Oncogenes Growth-factor receptors: Cellular growth factors stimulate growth by binding to a growth-factor receptor at the cell membrane The binding activates a signal transduction pathway that acts through Ras, cyclin D, and its partner CDKs. Amplification and overexpression of the gene that encodes the receptor for epidermal growth factor (EGFR) has been found in many malignant astrocytomas, glioblastomas, breast and ovarian cancers, head and neck cancers and melanomas.

30 30 Oncogenes Ras: The Ras protein acts as a switch in stimulating cellular growth in the presence of growth factors Certain mutant Ras proteins lack GTPase activity and remain in the form of Ras–GTP. The signal for cellular growth is transmitted constitutively--unrestrained growth and division take place Fig

31 31 Tumor-Suppressor Genes Tumor-suppressor genes normally negatively control cell proliferation or activate the apoptotic pathway Loss-of-function mutations in tumor-suppressor genes contribute to cancer progression.

32 32 p53: Loss of function of p53 eliminates the DNA checkpoint that monitors DNA damage in G1 and S The damaged cells survive and proliferate and their genetic instability increases the probability of additional genetic changes and thus progression toward the cancerous state p53 proves to be nonfunctional in more than half of all cancers Mutant p53 proteins are found frequently in melanomas, lung cancers, colorectal tumors, bladder and prostate cancers, and astrocytomas. Tumor-Suppressor Genes

33 33 p21: Loss of p21 function results in renewed rounds of DNA synthesis without mitosis, and the level of ploidy of the cell increases. Mutations in the p21 gene occur in some prostate cancers. p16/p19ARF: The p16 and p19ARF proteins are products of the same gene transcribed from different promoters The p16 product can inhibit the cyclin D–Cdk4 complex and help control entry into S phase The gene is deleted in many gliomas, mesotheliomas, melanomas, nasopharyngeal carcinomas, biliary-tract and esophageal carcinomas. Tumor-Suppressor Genes

34 34 RB: The retinoblastoma protein controls the transition from G1 to S phase by controlling the activity of the transcription factor E2F Loss of RB function frees E2F hence excessive rounds of DNA synthesis are continuously being initiated. Loss of RB function is found in melanomas, small-cell lung carcinoma, osteosarcoma and liposarcomas Tumor-Suppressor Genes

35 35 Bax: The Bax tumor-suppressor protein promotes apoptosis Loss of Bax function is found particularly in gastric adenocarcinomas and in colorectal carcinomas associated with microsatellite instability because of defective mismatch repair. Cells that are defective in mismatch repair are prone to undergo replication slippage leading to deletions or additions of nucleotides in runs of short tandem repeats Tumor-Suppressor Genes

36 36 Bub l: Bub1 is a protein that is primarily involved in the spindle checkpoint A subset of colon cancers show chromosomal instability. Some of these unstable lines are defective in Bub1. Tumor-Suppressor Genes

37 37 Familial Cancers Mutations that predispose to cancer can be inherited through the germ line The presence of this mutation predisposes the individual to cancer, because it reduces the number of additional somatic mutations necessary for a precancerous cell to progress to malignancy

38 38 Li–Fraumeni Syndrome The Li–Fraumeni syndrome shows clear autosomal dominant inheritance. However, the affected individuals have a range of different tumors and often have more than one, including osteosarcoma, leukemia, breast cancer, lung cancer, soft- tissue sarcoma, and brain tumors A large fraction of Li–Fraumeni families show segregation for a mutation in the p53 gene. A situation analogous to the human Li–Fraumeni syndrome has been created in mice by experimental knockout (loss of function) of the p53gene via the germ-line transformation

39 39 Fig

40 40 Retinoblastoma RB protein in animal cells holds cells at restriction point by binding to and holding E2F Alfred Knudson in 1971 suggested that loss of the wildtype allele of a tumor-suppressor gene might be the triggering event at the cellular level for tumors in heterozygous genotypes

41 41 Retinoblastoma Knudson suggested that genesis of a tumor in familial cases of RB required a single hit in a somatic cell, whereas genesis of a tumor in sporadic cases required two hits RB is inherited in pedigrees as a simple Mendelian dominant. But Knudsons hypothesis implied that even in familial cases, there must be another mutational event that triggers tumor development. Analysis of genetic markers around the gene in tumor cells revealed that the triggering event is the loss of the wildtype RB1 allele

42 42 At the organismic level, mutant gene is dominant. At the cellular level, it is recessive. Several mechanisms can uncover the mutant allele: chromosome loss, mitotic recombination, deletion, and inactivating nucleotide substitutions Uncovering of the recessive allele by various mechanisms is called loss of heterozygosity Retinoblastoma (RB) is an inherited cancer syndrome associated with loss of heterozygosity in the tumor cells. Retinoblastoma

43 43 Defects in DNA Repair Genetic instability clearly contributes to the origin of tumor cells Some inherited cancer syndromes result from defects in processes of DNA repair Inherited skin cancer syndromes are called xeroderma pigmentosum Xeroderma pigmentosum cells are defective in nucleotide excision repair Individuals with this syndrome are very sensitive to ultraviolet light

44 44 Acute Leukemias Acute leukemias are malignant diseases of the bone marrow, spleen, and lymph nodes associated with uncontrolled proliferation of leukocytes and their precursors in the bone marrow Acute leukemias do not arise as a consequence of alterations in cell cycle regulation or checkpoints, nor are they familial Up to 60% of acute leukemias result from a chromosomal translocation that fuses a transcription factor with a leukocyte regulatory sequence

45 45 Acute Leukemias The translocations are of the two types: promoter fusionthe coding region for a gene that encodes transcription factor is translocated near an enhancer for an immunoglobulin heavy-chain gene or a T-cell receptor gene gene fusionfound more frequently in acute leukemia than a promoter fusion. the translocation breakpoints occur in introns of genes for transcription factors in two different chromosomes. The result is a fusion gene called a chimeric gene composed of parts of the original gene

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