Origin of Spliced-Leader trans-splicing C. elegans SL1 RNA U1 snRNA TMG SM
SL trans-splicing in C. elegans 70% of C. elegans genes are SL1 trans-spliced Loss of SL1 RNA gene is embryonic lethal Many questions remain – how are trans-splicing and cis-splicing coordinated in the same cell? – How is the SL RNA brought to the 5’end of the mRNA? – How are SM proteins recycled from the SL introns?
An in vivo assay for SL trans-splicing GFP SL1 GFP ATG outron SL trans-splicing
Screening for molecules required for SL trans-splicing GFP ATG outron Control mRNA
Summary Developed in vivo assay to visualise defects in SL trans-splicing First ever systematic genetic screens for molecules required for SL trans-splicing in any organism SL trans-splicing an ideal target for the development of novel anti-helminthics: – Not present in plants or vertebrates