Presentation is loading. Please wait.

Presentation is loading. Please wait.

Nikko Bacalzo Douglas Dy Jeremy Ly France Tan. Instrument Used: Ion Chromatography.

Similar presentations


Presentation on theme: "Nikko Bacalzo Douglas Dy Jeremy Ly France Tan. Instrument Used: Ion Chromatography."— Presentation transcript:

1 Nikko Bacalzo Douglas Dy Jeremy Ly France Tan

2 Instrument Used: Ion Chromatography

3 http://toxicfreekidsblog.org/2011/07/toxic-toothpaste/ http://www.webmd.com/oral-health/image-collection-oral-health#phototake_rm_photo_of_tooth_illustration.jpg

4 http://www.skepticnorth.com/2010/10/fluoridiated-water-debate-returns-nothing-new-is-said/ http://www.fluoridation.com/teeth.htm

5

6 http://www.separations.us.tosohbioscience.com/ServiceSupport/TechSupport/ResourceCenter/PrinciplesofChromatography/IonExchange

7 Injection valve Readout Mobile phase/ solvent reservoir Pump Column Detector Excess/ waste Filter

8

9 Fluoride calibration curve

10 Chloride calibration curve

11 Brand Ionn %(w/w) mean ± std dev%RSD Sensodyne F3-- Cl2 a 0.00923 ± 0.000020.270 Colgate F3-- b -- Cl3-- Hapee F3-- Cl 2 a 0.016 ± 0.00215.3 Close-Up F3-- Cl3--

12 conc (ppm) spiked (mg) unspiked (mg) added (mg) %recov ery SPIKE40.622.0310.020521.344149.6

13 Four toothpaste brands were analyzed for their fluoride and chloride contents (Sensodyne, Colgate, Close-Up and Hapee). Standards were run in the ion chromatograph, and the retention times and the concentration-peak area relationships of each were drawn from the data. Out of which only Sensodyne and Hapee showed quantifiable amounts of chloride in the toothpaste formulation. Calculation yielded concentrations of 0.00923% and 0.016% chloride in both toothpastes, respectively. Other samples had no sufficiently quantifiable amounts of both anions, suspected to be consequence of matrix effect.

14 Use other MP: carbonate/bicarbonate buffer, KOH Use gradient elution Standard addition instead of calibration curve Homogenization of samples Analyze other anions (MFP, benzoate, sulfate, phosphate) Method validation

15 Takahara, Y, et al. Photooxidation of organic compounds in a solution containing hydrogen peroxide and TiO 2 particles under visible light. Journal of Applied Chemistry. Vol.35, 793-797. Shimadzu Corporation. The UV-2401PC: A Superbly Engineered Instrument. Accessed on May 21, 2012.

16


Download ppt "Nikko Bacalzo Douglas Dy Jeremy Ly France Tan. Instrument Used: Ion Chromatography."

Similar presentations


Ads by Google