1. MICROBIOLOGIC DX. 3 FORMS : ANTIGENS ANTIBODIES SUGESTIVES TESTS

2. ANTIGENS MICROSCOPIC OBSERVATION salin solution a) wet prep dark field indian ink

3. Ags. gram stain acid fast stain b) fix prep giemsa hematoxilin argentic stain

4. Ags.: Bacterian identification. A) cultures: B.A., EMB, SS, LOWENSTEIN B) tissues cultures C) animal inoculations

5. ags Molecular studies: DNA, probes, Pcr, western-blot, chemioluminiscency

6. antibodies A) specific tests B) inespecific tests

7. ANTIBODIES Meanings: has, had, o cross reaction. fever reaction: Widal, Hudlesson and Weil-Felix ASLO: ANTISTREPTOLISINS VDRL: VENERAL DISEASE REACTION LABORARATORY

8. ABS IG M: TOXOPLASMOSIS (SABIN – FELDMAN) IN NEW BORNE = INFECTION IG G: TOXOPLASMOSIS IS MOTHER’S IG G

9. INESPECIFIC EXAMPLES WEIL-FELIX VDRL EB TEST

10. RUTIN TESTS GENERAL TESTS C.B.C. RED CELLS W.B.C. INCREASE: ACUTE Number bacterial INFECTION NORMAL O DECREASE: chronic INFECTION Platelets: decrease gram negative infection.

11. WBC DIFERENTIAL COUNT NEUTROPHILS INCREASE: ACUTE INFECTION LYNPHOCYTES INCREASE: CHRONIC o VIRAL INFECTION

12. OTHERS LIKE: CSF o UA. Pleocytosis (leucocytosis spinal fluid) = infection. PMN = ACUTE INF. MN = CHRONIC or VIRAL INF. Leucocyturia = UTI

13. Clinical case NURSE 30 YEARS OLD. SUPERVISOR ( NIGHT ) “ RAMON GARIBAY “ HOSPITAL, FEVER ( 39 ) HEADACHE DURING 2 WEEKS. Fever reaction (+ )‏ 1:640 (WEIL-FELIX). TX. AMPICILIN

14. Normal flora 1.- Why flora = most of microorganisms are bacterías. 13 2.- How many cells are in the body?:10 14 bacterias : 10

15. flora Skin, nose,mouth intestinal, urogenital