Presentation on theme: "Setting up a Molecular Lab in a Developing Country"— Presentation transcript:
1Setting up a Molecular Lab in a Developing Country Dr. Azhar HussainResearch AssociateMolecular Oncology Unit.King Fahad National Center for Children’sCancer and Research.King Faisal Specialist Hospital &Research center.Riyadh, Saudi Arabia.
2Translational Research Since the emergence of Translational Research (TR), the term, “from bench to bedside” has taken a new meaning in the management of cancerTranslational research (TR) involves both, the development of novel diagnostics and therapeutics.
3Translational Research Novel Diagnostics Results achieved in research laboratories have direct implications on clinical diagnosis, treatment and prognosis of cancer todayMonitoring of patients for minimal residual disease during and after treatment using molecular markersDetection of chromosomal abnormalities using PCR techniques
4Translational Research Novel Therapeutics New Drug DiscoveryGleevic (STI571) for the treatment of CMLRadio labeled Monoclonal antibodies for metastatic medullary thyroid cancerTrastuzumab (Herceptin) for the treatment of HER- 2’ (ErbB-2)-over-expressing metastatic breast cancer
5Translational Research Novel Therapeutics On-going trials with drugs or naturally occurringcompounds.PI3’-kinase pathway inhibitor (LY294002)Jak-Stat pathway inhibitor (Ag490)Curcumin (PI3’-kinase and Jak-Stat pathway inhibitor)
6Why is a Molecular biology lab needed in a developing country? Advances in the understanding of the biology of cancerhave increased the cure rate of selected cancers indeveloped countries.Developing countries continue to lack far behind.Better understanding of the biology of cancers is neededA functional molecular biology laboratory is vitalin developing countries to ensure accurate diagnosisand appropriate treatment.
7Three essential components for establishing a molecular biology lab. 1: Adequate and well organized laboratoryspace2: Essential and specialized labequipment with proper storage capacity3: Well-trained staff including lab chief,supervisors, post-docs and technicians
8Adequate and well organized laboratory space Long hallways can be utilized for storage as well as forwork benches for different procedures and experimentsThe flow of a lab should ALWAYS be in one direction
9One way road. No U-turn allowed!!! Pre-PCR areaTemplate areaPCR area
10One way road. No U-turn allowed!!! Pre-PCR areaPCR areaTemplate areaGel running area
18Machines cannot replace the human mind. Machines and instruments do not run on their ownMachines and instruments do not run researchers and scientistsResearchers and scientists run these machines
19Molecular biology lab in a developing country The same Quality of work can be achieved with essential lab equipment in a developing countryProper planningClear goalsExpertise in the molecular biology fieldAdequate fundingDedication of lab personnel
20All that glitters is not Gold!!! Third and most important component of establishing a good molecular biology labWell-trained staffHead of the labSupervisorspost-docstechnicians.
21Head of the Lab Should be an experienced scientist (MD or PhD) Up to date in molecular techniques and latest scientific literatureHas a strong track record for funding and publicationsHas creative ideas for researchDiplomatic and patientHas good rapport with the staff
22Supervisor and lab manager Responsible for standardization of molecular biology techniques and protocolsConforms to international standards for all techniques e.g.DNA isolation, RNA isolation and protein extractionSets up projects for post-docs and techniciansSupervises staffOrders reagents and consumables for the labMay have an independent project of his/her own
23Post-docsManage and execute independent projects in the lab with minimal supervisionTake care of the daily bench workrunning experimentscollecting dataSearch relevant scientific literatureWrite abstracts and papers on results achieved in the lab
24TechniciansHelp the post-docs and scientists in different projects and experimentsResponsible for all the standard work in the lab such DNA, RNA and protein isolationCan also be trained to run more involved experiments e.g.western blots, PCRHelp in collecting data that is generated by these experiments
25Training Requirements for all lab personnel Universal precautionsLab safety courseRadiation safety courseMolecular biology updatesHuman subject training
26ConclusionsShould a Molecular lab be established in a developing country?YesHow?private and governmental fundingInternational, national and regional collaborationsDedicated and qualified personnelBasic lab equipmentCOMMITMENT TO RESEARCH!
27King Fahad National Center for Children’s Cancer & Research