Presentation on theme: "Calcium-butyrate SINTOBUTYL Product and specifications"— Presentation transcript:
1Calcium-butyrate SINTOBUTYL Product and specifications
2Calcium-butyrate : Product and specifications Sintobutyl- Chemically pure calciumbutyrate (slight surplus of Ca) – fine powderDoesn’t smell if not touched with humid surfaces (hands, ...).May partly dissociate in contact with humidity and release unpleasant smellMin % butyrate-ANION and approximately 17% Calcium
3 Fat components from vegetable origin SintobutylCoating: incapsulates the active compound (Ca-butyrate) Fat components from vegetable origin( Very good nutritional properties)Fatty acids with high melting pointHydrogenated fatty acids :C14 and lower (%) 1 %C16 palmitic acid (%) 45 %C18 stearic acid (%) 53 %C18’oleic acid (%) 0.5 %C20 and higher %
4How Sintobutyl ranks between other claimed alternatives ? Sintobutyl (calcium butyrate) and Antibiotic Growth PromotersHow Sintobutyl ranks between other claimed alternatives ?
5Sintobutyl versus Other “Gut Modifiers” “Gut Flora Stabilisers” Sintobutyl versus Other “Gut Modifiers” “Gut Flora Stabilisers” Are they all the same ?Encapsulated Yeast Components Botanical extractsFeature / Effect Calcium-butyrate (MOS, hydrolysed yeast) (herbs, roots,fruits)Probiotics,Prebiotics, enzymes acidifiers “coated”)GUT Morphology& Functioning(villi, epithelium, mucosa)IN-DIRECT EFFECT(some even stimulate butyric acid, -producing bacteria)DIRECTAGENT / NUTRIENT+ and -GUT Micro floraBacterial Load Metabolism, Pathogens (C-Cl-Salm)IN-DIRECT EFFECTAttachmentVFA –production (BA)Substrate interactionDIRECT INHIBITIONLow MIC-value’s at intestinal pH = In vivo flora studiesDIRECT / INDIRECT EFFECT+ and -
6More Feed Additives claim, support BUTYRATE effects Protection and regeneration of intestinal villi (mucosa integrity)Non Digestible OligosaccharidesF.O.S. (inuline, profeed)M.O.S. (yeast cell walls)Bacterial shift (LAB, Bifido)Fermentation to VFA and more Butyric (Megasphera LA -> BA)Selective bacterial inhibition at intestinal, ceacal level : Coli, Clostridium, SalmonellaButyrate(butyric acid)Immuno-stimulantStimulation of enzyme synthesisBacteria attachment (lectins)Some induce BA production ()Herbal ExtractsStimulation of immunityZootechical effects : FI, DWG, FCR, Egg Shell strengthPart is converted at intestinal level to ButyricMCFA’sSo, why not adding butyrate (Sintobutyl) directly !!??!!
7Mode of action of PROGUT (Vuorenmaa J & Vaahtovua J. (2006) PROGUT = Hydrolysed brewery yeast product;Prebiotic effects + Yeast Cell Wall effects are claimedStabilize gut floraSupport natural immunityPrevent attachment of E.Coli, Salmonella to mucusIncreases the MBI with piglets and poultry !!!Bifidobacteria + butyric acid producing bacteriaMICROBIAL BALANCE INDEX =Enteric group bacteria + bacteroidesSignificant correlation with DWG and FCRMeans more Butyrate in the gut, so just give straight Sintobutyl !!!MBI
9Comparative mode of action and benefits of : ABGP ButyProl Probiotics ButicalGut integrityEnzyme synthesisImmunityMicrobial Shift (beneficial)Metabolic activity floraInhibition - Coli- Clostridium- Salmonella- / /-/-indirect direct indirect++ / /+- - / /+Digestibility of nutrientsAbsorptionFCRIntestinal disordersPathogensLitter qualityindirect/+- - / /+- /
10Sintobutyl versus Aromabiotic (medium chain fatty acids) in rabbits
11Totally untrue !!!!!!!!!!! Pure Marketing !!!!! Sept 06 : so far limited use in 2-3 countries of EU-25 and on the way back (“fashion”)
12Ca-butyrate (Sintobutyl) versus MCFA’s CLAIM-ACTIVITY MCFA CalciumbutyrateMCFA soluble both in water and lipids, which would influence the speed of penetration of the product through the bacterial cell wall. Molecules have medium length C10-C14 and may be quite big to penetrate.Bacterial InhibitionE. ColiClostridiumVilli integrityPerformance (dwg,fcr)Butyric acid is also soluble in water and in fat, both lipofylic and hydrofylic. On top the molecule is smaller (C-4), which normally could be easier to penetrate.log reduction (10-15 days) at 0.2 % - MIC = 0.5% !!log reduction (25-50 mmol) – J . Decuyper 2003-U.G.MCFA would inhibit lipase production by bacteria, which seems needed for bacteria to attach to the gutwall. So attachment may be hindered and bacteria lessivate !? INDIRECT effectCalciumbutyrate has a DIRECT inhibition effect at pH=6 on all pathogenic clostridium strains (A.Decostere). Inhibtion of anaerobes by 0.5 log (J. Decuyper)MCFA would Increase villi length and vili/crypt ratio, but this is again INDIRECT (as more additives may do by fermentation or transformation to butyric acid)Butyrate is the FUEL (nutrient) of the gutwallcell (villi), having a DIRECT effect on the gutwall integrity and regeneration. Besides systemic effect, also the topical effect (local)Effects seems limited, nihil to negative (on feed intake). Few data !Significant Positive Effect (higher feed intake -attractant). Full data base !
13Butyrate – MCFA’s : similarities & differences !? Property, advantage MCFA’s Butyrate (butyric acid) Specific CharacteristicsChain length caproic C6, Caprylic C-8, Capric C C4 (longest SCFA)pK-valueSolubility fat and water soluble fat and water solubleME kJ/gr kJ/grHuman application as functional lipid (“slimming”) as anti-cancer (colon)Absorption enhancing (paracellular permeability) enhancing (idem +emulsifyer)Bacterial Inhibition mg/mlE. Coli > < 5.0 (UG-AF)Salmonella > > < 5.0 (UG-VF)Clostridium > (UG-VF)C-12 =
14Butyrate – MCFA’s : similarities & differences !? Property, advantage MCFA’s Butyrate (butyric acid) Specific CharacteristicsKetosis effects (negative !!) Risk : C6-C8 in neonatals (piglets) not (only in ruminants)Association with C-4, VFA Antagonist (drop in C-4, VFA) Synergistic (= or >)=>Compatible with FOS,YCW, NOT YESSome Probiotics, ....Risk of lipolysis in feed Real NOT(lipase)Required antimicrobial gr/l pure, stomach, gut concentrationFI piglet 300 g/d => kg per ton pureX 2 = kg per ton of feed kg/tonAccumulation in fat at high dosages (4 %) NOT
15MCFA’s = Butyrate antagonist !!!!! – Conflicting – Worrying ??? J. Goris (2006), ILVO : C8 or caprylic acid changes fermentation profile in caecum of piglets : increase lactate, decrease butyrate and propionate (reversible)F.Boyen & Pasmans (2006) – Effect on salmonella invasion of caprylic and caproic acid + butryic acid is antagonistic. Butyric alone decreases invasion !Leeson & Antongiovanni (2005) – Triglycerides of mcfa + butyric less effective in broilers than Triglycerides of butyric acid alone.Van Oeckel (2005) - ILVO – Piglets trial : performance of combination of mcfa-product + butyrate less good than mcfa, butyrate, but also than negative control.Maertens L (2006) – ILVO – Rabbits : mcfa-product decreased significantly VFA’s and Butyric concentration in caecum. Unbalancing cecal flora !!!
16Salmonella invasion through epithelial cells of broiler chickens after treatment with butyric acid or MCFA (caproic acid)IN VITRO !!“MCFA seem to decrease invasion at least to the same extent as butyric acid but at lower concentrations”F. Van Immerseel et All (2004) – Appl. Environ.Microbiol. 6/2004 :In Vitro data : what happens in intestine with additive ? How much caproic acid is liberated from coconut-oil ?Related only to eventual accumulation in liver and spleen, not in caecum (see in vivo test)
17a b a’ a’ a” a” a b a’ b’ a” a” Salmonella in different organs of broiler chickens after treatment with butyric acid or MCFA (caproic acid)Log cfu/grIN VIVO !!-1.66 log-2.2 loga b a’ a’ a” a” a b a’ b’ a” a”= 3 kg Butical 60= 6-30 kg MCFA-product ??Inoculation with 10exp3 Salmonella Enteritidis on day 5, counts on day 8 (Van Immerseel - University Ghent)
18Composition of a commercial MCFA product Method : saponification BF3/MeOH (hot) BF3/MEOH (cold)+ BF3/MeOH (hot)Fatty Acid % rel g/100 gram % rel g/100 gram % rel g/100 gramCaproic C6:Caprylic C8:Capric C10:Undecanoic C11:Lauric C 12:Sample from Belgium – 2004 ; Independent industrial laboratory, analysis in 2005