Presentation on theme: "Calcium-butyrate SINTOBUTYL Product and specifications."— Presentation transcript:
Calcium-butyrate SINTOBUTYL Product and specifications
Calcium-butyrate : Product and specifications - Chemically pure calciumbutyrate (slight surplus of Ca) – fine powder - Doesnt smell if not touched with humid surfaces (hands,...). - May partly dissociate in contact with humidity and release unpleasant smell - Min % butyrate-ANION and approximately 17% Calcium Sintobutyl
C oating: incapsulates the active compound (Ca-butyrate) Fat components from vegetable origin ( Very good nutritional properties) Fatty acids with high melting point Hydrogenated fatty acids : C14 and lower (%) 1 % C16 palmitic acid (%)45 % C18 stearic acid (%)53 % C18oleic acid (%)0.5 % C20 and higher 0.4%
Sintobutyl (calcium butyrate) and Antibiotic Growth Promoters How Sintobutyl ranks between other claimed alternatives ?
Sintobutyl versus Other Gut Modifiers Gut Flora Stabilisers. Are they all the same ? GUT Morphology & Functioning (villi, epithelium, mucosa) IN-DIRECT EFFECT (some even stimulate butyric acid, -producing bacteria) DIRECT AGENT / NUTRIENT IN-DIRECT EFFECT + and - GUT Micro flora - Bacterial Load - Metabolism, - Pathogens (C-Cl-Salm) IN-DIRECT EFFECT - Attachment - VFA –production (BA) - Substrate interaction DIRECT INHIBITION Low MIC-values at intestinal pH = 6 In vivo flora studies DIRECT / INDIRECT EFFECT + and - Encapsulated Yeast Components Botanical extracts Feature / Effect Calcium-butyrate (MOS, hydrolysed yeast) (herbs, roots,fruits) Probiotics,Prebiotics, enzymes acidifiers coated)
More Feed Additives claim, support BUTYRATE effects Butyrate (butyric acid) Protection and regeneration of intestinal villi (mucosa integrity) So, why not adding butyrate (Sintobutyl) directly !!??!! Selective bacterial inhibition at intestinal, ceacal level : Coli, Clostridium, Salmonella Stimulation of immunity Stimulation of enzyme synthesis Zootechical effects : FI, DWG, FCR, Egg Shell strength Non Digestible Oligosaccharides Herbal Extracts MCFAs F.O.S. (inuline, profeed) M.O.S. (yeast cell walls) Bacterial shift (LAB, Bifido) Fermentation to VFA and more Butyric (Megasphera LA -> BA) Immuno-stimulant Bacteria attachment (lectins) Some induce BA production () Part is converted at intestinal level to Butyric
Mode of action of PROGUT (Vuorenmaa J & Vaahtovua J. (2006) PROGUT = Hydrolysed brewery yeast product; Prebiotic effects + Yeast Cell Wall effects are claimed - Stabilize gut flora - Support natural immunity - Prevent attachment of E.Coli, Salmonella to mucus Increases the MBI with piglets and poultry !!! MICROBIAL BALANCE INDEX = Bifidobacteria + butyric acid producing bacteria Enteric group bacteria + bacteroides Significant correlation with DWG and FCR MBI Means more Butyrate in the gut, so just give straight Sintobutyl !!!
Sintobutyl versus Aromabiotic (medium chain fatty acids) in rabbits
Totally untrue !!!!!!!!!!! Pure Marketing !!!!! Sept 06 : so far limited use in 2-3 countries of EU-25 and on the way back (fashion)
Ca-butyrate (Sintobutyl) versus MCFAs CLAIM-ACTIVITY MCFA Calciumbutyrate Bacterial Inhibition E. Coli Clostridium Villi integrity Performance (dwg,fcr) MCFA soluble both in water and lipids, which would influence the speed of penetration of the product through the bacterial cell wall. Molecules have medium length C10-C14 and may be quite big to penetrate. Butyric acid is also soluble in water and in fat, both lipofylic and hydrofylic. On top the molecule is smaller (C-4), which normally could be easier to penetrate log reduction (10-15 days) at 0.2 % - MIC = 0.5% !! log reduction (25-50 mmol) – J. Decuyper 2003-U.G. MCFA would inhibit lipase production by bacteria, which seems needed for bacteria to attach to the gutwall. So attachment may be hindered and bacteria lessivate !? INDIRECT effect Calciumbutyrate has a DIRECT inhibition effect at pH=6 on all pathogenic clostridium strains (A.Decostere). Inhibtion of anaerobes by 0.5 log (J. Decuyper) MCFA would Increase villi length and vili/crypt ratio, but this is again INDIRECT (as more additives may do by fermentation or transformation to butyric acid) Butyrate is the FUEL (nutrient) of the gutwallcell (villi), having a DIRECT effect on the gutwall integrity and regeneration. Besides systemic effect, also the topical effect (local) Effects seems limited, nihil to negative (on feed intake). Few data ! Significant Positive Effect (higher feed intake -attractant). Full data base !
Butyrate – MCFAs : similarities & differences !? Property, advantage MCFAs Butyrate (butyric acid) Specific Characteristics Chain length caproic C6, Caprylic C-8, Capric C10 C4 (longest SCFA) pK-value Solubility fat and water soluble fat and water soluble ME 26.6 kJ/gr 17.4 kJ/gr Human application as functional lipid (slimming) as anti-cancer (colon) Absorption enhancing (paracellular permeability) enhancing (idem +emulsifyer) Bacterial Inhibition mg/ml E. Coli > < 5.0 (UG-AF) Salmonella > 5 3 > 5 < 5.0 (UG-VF) Clostridium > (UG-VF) C-12 =
Butyrate – MCFAs : similarities & differences !? Property, advantage MCFAs Butyrate (butyric acid) Specific Characteristics Ketosis effects (negative !!) Risk : C6-C8 in neonatals (piglets) not (only in ruminants) Association with C-4, VFA Antagonist (drop in C-4, VFA) Synergistic (= or >) =>C ompatible with FOS,YCW, NOT YES Some Probiotics,.... Risk of lipolysis in feed Real NOT (lipase) Required antimicrobial 3-5 gr/l pure, stomach, gut concentration FI piglet 300 g/d => kg per ton pure X 2 = kg per ton of feed kg/ton Accumulation in fat at high dosages (4 %) NOT
MCFAs = Butyrate antagonist !!!!! – Conflicting – Worrying ??? 1)J. Goris (2006), ILVO : C8 or caprylic acid changes fermentation profile in caecum of piglets : increase lactate, decrease butyrate and propionate (reversible) 2)F.Boyen & Pasmans (2006) – Effect on salmonella invasion of caprylic and caproic acid + butryic acid is antagonistic. Butyric alone decreases invasion ! 3)Leeson & Antongiovanni (2005) – Triglycerides of mcfa + butyric less effective in broilers than Triglycerides of butyric acid alone. 4)Van Oeckel (2005) - ILVO – Piglets trial : performance of combination of mcfa- product + butyrate less good than mcfa, butyrate, but also than negative control. 5)Maertens L (2006) – ILVO – Rabbits : mcfa-product decreased significantly VFAs and Butyric concentration in caecum. Unbalancing cecal flora !!!
Salmonella invasion through epithelial cells of broiler chickens after treatment with butyric acid or MCFA (caproic acid) IN VITRO !! MCFA seem to decrease invasion at least to the same extent as butyric acid but at lower concentrations F. Van Immerseel et All (2004) – Appl. Environ.Microbiol. 6/2004 : In Vitro data : what happens in intestine with additive ? How much caproic acid is liberated from coconut-oil ? - Related only to eventual accumulation in liver and spleen, not in caecum (see in vivo test)
Salmonella in different organs of broiler chickens after treatment with butyric acid or MCFA (caproic acid) Log cfu/gr a b a a a a a b a b a a Inoculation with 10exp3 Salmonella Enteritidis on day 5, counts on day 8 (Van Immerseel - University Ghent) -2.2 log log = 6-30 kg MCFA-product ?? = 3 kg Butical 60 IN VIVO !!