Presentation on theme: "Clinical pathology: SEROMARKER FOR TB"— Presentation transcript:
1Clinical pathology: SEROMARKER FOR TB GENERAL OBJECTIVE:After finishing lab activity of Seromarker TB, the student will be able to describe the Seromarker TB in the DMS case problemSPECIFIC OBJECTIVE:At the end of lab activity of Seromarker TB, the student will be able to interprete:- the normal of Seromarker TB- the abnormal of Seromarker TB
2Introduction Tuberculosis : Chronic infectious disease M. tuberculosis involves multiple organs>> lungIndonesia: number 3 in the worldafter India and ChinaWest Java Health Profile 2000:The caused of morbidity (no 4) in the hospitalNumber 2 the caused of mortality
4Diagnosis:- Direct smear:Ziehl Nielson: Acid fast bacilli- Culture: GOLD STANDARD- Tuberculin test- Chest X ray (Pulmonary TB)
5Mycobacterium tuberculosis Problem?Culture: 4-6 weeksNot available in all center/laboratoryExtra pulmonary TBSmear negative Pulmonary TBChildrenSEROMARKER TB/Immunological testDetection of antibodies againts TB
6Seromarker TBAnti Myc. TB ELISAICT TB- A chromatographic immunoassay for qualitative detection of antibodies againts TB in human serum or plasma- The test employing recombinant antigen38kD, 16 kD and 6 kD (ESAT-6),lipoarabinomannan- The use of a collection of antigen improves the sensitivity of serological test
7Seromarker TBMycotec TB( recombinant) employs the complexes conjungate with gold colloidal particle passes over the immobilized multiple of recombinant TB ag precoated on the test area (T) once the patient sample is added to the sample well.
8Principle of the testIf there are some ab to TB are present in patient sample then the TB recombinant ag capture them, a pink/purple band is produced on the test area.
9The migration of remaining complexes which were not bound by those TB ab, will be then immobilized to the control area (C).A pink/purple band is appeared on control area, the band on control performs a properly test
10Prosedur Pengujian Interpretasi Hasil 1. Pipetkan 100 μl serum atau plasma2. Baca hasil 5-20 menit setelah penetesan spesimenInterpretasi HasilPositif Tampak 2 garis warna merah muda atau ungu di area Test (T) dan Kontrol (C)Negatif Tampak 1 garis warna merah muda atau ungu di area Kontrol (C)Invalid Tidak tampak garis di area Kontrol (C)