Presentation on theme: "Part II. Tandem MS. Mass filter; complete spectrum is obtained by scanning whole range Ions are lost Mass range 10- 4,000 Da Mass Analyzer (2) – Quadrupole."— Presentation transcript:
Sample Preparation tissue fraction gel peptides Add trypsin MPSER …… GTDIMR PAK …… HPLC To MS/MS
Tandem Mass Spectrometer Quadrupole mass analyzer collision parent ionsfragment ions MPSER SG… + PAK + + P + AK PAK + + PA + K AK + P K + PA P + K + + AK + PAK + + peptide sequencing … TOF mass analyzer ions detector ESI QTOF
LGSSEVEQVQLVVDGVKpeptide sequence: tandem mass spectrometry: MS/MS spectrum de novo sequencing: LGSSEVEQVQLVVDGVK database
Database Search Methods Mascot – matrix sciences – General software Sequest – John Yates et. al. – Distributed by Thermo Finnigan. – Works for Thermo’s LTQ. PEAKS – Bin Ma et. Al. – Distributed by Bioinformatics Solutions Inc. – General software
De Novo Sequencing (Dancik et al., JCB 6:327-342.) – Given a spectrum, a mass value M, compute a sequence P, s.t. m(P)=M, and the matching score is maximized. We consider the matching score of P is the sum of the scores of the matched peaks. De Novo Sequencing
Spectrum Graph Approach Convert the peak list to a graph. A peptide sequence corresponds to a path in the graph. Bartels (1990), Biomed. Environ. Mass Spectrom 19:363-368. Taylor and Johnson (1997). Rapid Comm. Mass Spec. 11:1067-1075. (Lutefisk) Dancik et al. (1999), JCB 6:327-342. Chen et al. (2001), JCB 8:325-337. ……
Difficulties Spectrum graph approach has difficulties to handle errors: – Missing of ions – break a path. – Too many peaks in a small error tolerance – too many edges connecting to the same peak. (reduce efficiency) – Error accumulation. – A peak is used as both a y-ion and a b-ion. It is still possible to solve these problems under the spectrum graph schema – E.g. The y-b overlap problem had been addressed by Dancik et al (1999) and Chen et al. (2001). – But things are getting complicated. – A reliable signal preprocessing is required.
PEAKS’ approach It is more natural and easier to handle the errors and noises. – Less dependent to the signal preprocessing. – Solved the missing ions and y-b overlap problems naturally. – Showed great success on real-life lab data. – Has been licensed by tens of research labs in public and private sectors.
Comparison LCQ data (Iontrap instrument): – Generously provided by Dr. Richard Johnson. 144 spectra. Micromass Q-Tof data: – Measured in UWO’s Protein ID lab. 61 spectra Sciex Q-Star data: – Provided by U. Victoria’s Genome BC Proteomics Centre. 13 good/okay spectra.
PEAKS v.s. Lutefisk completely correct sequences: –38/144 v.s. 15/144 correct amino acids: –1067/1702 v.s. 767/1702 v.s. partially correct sequences with 5 or more contiguous correct amino acids: –94/144 v.s. 64/144
PEAKS v.s. Micromass PLGS completely correct sequences: –23/61 v.s. 7/61 correct amino acids: –559/764 v.s. 232/764 partially correct sequences with 5 or more contiguous correct amino acids: –50/61 v.s. 24/61
PEAKS v.s. Sciex BioAnalyst completely correct sequences: –7/13 v.s. 1/13 correct amino acids: –115/150 v.s. 86/150 partially correct sequences with 5 or more contiguous correct amino acids: –12/61 v.s. 7/61
PTM PTMs are important to the functions of proteins. There are more than 500 types of PTMs included in the unimod PTM database. For example: Reversible phosphorylation of proteins is an important regulatory mechanism. Many enzymes are switched "on" or "off" by phosphorylation and dephosphorylation. This is done by the structural change caused by the PTM.
Phosphorylation pSpTpY HH H S TY Monoisotopic mass change: PO 3 H = 79.966
PTM increases complexity Most protein databases do not have the PTM information. Therefore, when PTM is present, one has to try different PTM possibilities to match a peptide with a spectrum. For peptide LGSSEVTMVYLK, if only phosphorylation is considered, there are 16 possibilities. – What if there are 10 possible PTM sites? This type of PTMs are called variable PTMs.
Fixed PTM Some PTMs are know to present all the time. – These are called fixed PTM. Oxidation of M. Mass +16. – It happens automatically in the air. So people often make sure that all of the M are oxidized. carboxyamidomethyl cysteine (CamC). Mass +57.02 – These are added intentionally to break the disulphide bonds. Fixed PTMs are easier.
Variable PTM in DB Search and DeNovo For DB search, have to try different combinations. For De Novo, each variable PTM is like adding a new amino acid. – For example, if pS, pT, pY are variable, then instead of having 20 characters in alphabet, we have 23. – But too many variable PTMs will reduce the accuracy of the de novo sequencing.
Peptide Identification v.s. Protein Identification
Peptide sequencing Peptide sequencing digestion Proteins Peptides …… >protein A PAKGTIRHIHGCDKRGAPWPAS… >protein B MSERNHLREIIGNEVR…… >protein C LSIMQDKDYSASFIS…… >protein A PAKGTIRHIHGCDKRGAPWPAS… >protein B MSERNHLREIIGNEVR…… >protein C LSIMQDKDYSASFIS…… Proteins MS/MS Protein ID Peptides PAK MSER LSIMQDK HIHGCDK EIIGNEVR SIMQMDYSASFIS...... PAK MSER LSIMQDK HIHGCDK EIIGNEVR SIMQMDYSASFIS...... Peptides Common procedure for protein ID
Problems A peptide appears in several proteins. A protein family may share many peptides. – Usually only one of them is true. A protein may have only one peptide or two weak peptides, is it true or false positive? – The “one hit wonder”.
Estimate False Positives Suppose you have a score for each identified protein. You want to choose a score threshold T. – Score >T positive (keep) – Score <=T negative (discard) It is important to estimate the false positive rate for each given result. False Positive Rate – In statistics, FPR= #false positives/#negative results. – We care more about FPR = #false positives/#results reported as positives. Positive (prediction) Negative (prediction) Positive (reality) TPFN Negative (reality) FPTN The two definitions are different!
Decoy Database Method Choose a decoy database: – for example, reverse the database. Anything from this database is false. Search in a real database and a decoy database separately – For same T, if there are x proteins in the decoy database >T, then perhaps there are x false proteins in the real database with score >T. Threshold T, – real db has 497 proteins >T, – decoy db has 7 proteins >T, – False positive rate is 7/497 = 1.4%
Problems Only works for large dataset. – Not statistically significant when dataset is small. Does not care how many proteins are actually kept. – Keeping only the true results is not our only goal, we also want to keep as many as true results as possible. Decoy database is only good for validation and cannot substitute a good scoring method.
SPIDER – listen to both parties! The solution when there is no protein database and no perfect MS/MS. 兼听则明，偏听则暗
de novo sequencing EISGNEVR protein DB ESIGNEVR database search protein DB homology search ESIGSEVR PEAKS: Ma et. al, Rapid Comm. Mass Spec. 2003 SI PatternHunter: Ma, Tromp and Li, Bioinformatics. 2002 SPIDER: Han, Ma and Zhang, JBCB. 2005
Two purposes of our research 1.Given de novo sequence with errors, find homolog of the real sequence. (searching) 2.Using the de novo sequence and the homolog as input, compute the real sequence. (sequencing)
LSCFAV “Listen to both sides and you will be enlightened; Heed only one side you will be benighted.” EACFAV de novo DACFKAV homolog
Homology mutations Sequence alignment Also called edit distance EACF-AVQR DACFKAV-R
Common de novo sequencing errors same mass replacement AN? NA? GAG?
More formally Let Sequencing: Given de novo sequence X, homolog Z, find Y such that is minimized. Let Searching: search a database for Z such that d(X,Z) is minimized. X Y Z seqError editDist
How to compute d s (X,Y) Easily align X and Y together (according to mass). For each erroneous mass block with mass m i, define the cost to be Define X Y Z seqError editDist (denovo)X: LSCFAV (real) Y: EACFAV
How to compute d(X,Z) A multiple alignment can be built from alignments (X,Y) and (Y,Z). Lemma: Dynamic Programming! Let X Y Z seqError editDist (denovo) X: LSCF-AV (real) Y: EACF-AV (homolog)Z: DACFKAV
Four cases of the last Block (A)(B)(C) no sequencing error D(i,j) is the minimum of the four cases.
The algorithm for computing 1. for m from 0 to m(X) step Δ for i from 0 to |Z| for j from i to |Z| Time complexity:
The algorithm for computing d(X,Z) and Y 1. for i from 1 to |X| for j from 1 to |Z| 2. output D(|X|,|Z|) as d(X,Z). 3. backtracking to get the best middle sequence Y. Time complexity: Total time complexity:
Experiment 28 spectra from ALBU_BOVIN. PEAKS de novo sequencing gives 13 correct and 15 partially correct sequences SPIDER found good peptide homologues in human protein DB for all. 24 constructed correct peptide sequences. PEAKS EAEGNEVR human DB SPIDER ESIGSEVR ESIGNEVR ALBU_BOVIN 28 13+15 28 24+4
Two exemplary results (denovo) X: CCQ[W ]DAEAC[AF] K (real) Y: CCK AD DAEAC FA VE GP K (homolog)Z: CCK[AD]DKETC[FA] K (denovo) X: FVE LVTD[TL]K (real) Y: FVE VTK LVTD LT K (homolog)Z: FAE LVTD[LT]K homology mutations sequencing errors
Four modes in SPIDER Homology mode Non-gapped homology mode – Assume sequencing error and homology mutations do not overlap. Segment match mode – Assume no homology mutations. Exact match mode – Assume no sequencing errors or homology mutations.
Experiment 144 ion trap MS/MS spectra, lower quality spectra. The proteins are all in Swissprot but not in human database. PEAKS 2.0 was used to de novo sequence. SPIDER searches Swissprot and human databases, respectively.
People like SPIDER Best Paper Award at CSB2004 Some random emails we received – “I'm a big SPIDER fan!” Shinichi Iwamoto, Shimadzu Corporation – “The results I've been getting have been consistently very good. Thank you for this great piece of software!” Jason W. H. Wong, University of Oxford – “Your software is by far the fastest and more user-friendly I have found.” Juan Luis, University of Georgia – …… – I plan to teach SPIDER in my Advanced Bioinformatics class. I wonder if your powerpoint slides are available?” Pavel Pevzner, Ronald R. Taylor Professor of Computer Science, UCSD Included in PEAKS as both a separate tool and an intermediate step in protein candidates generation. The best is yet to come – People just started using the de novo + homology approach.