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Ca 2+ release mechanisms in cardiac & skeletal muscle OBJECTIVES Methodology:Methodology: Chimeric proteinsChimeric proteins FRETFRET Differences in Ca.

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Presentation on theme: "Ca 2+ release mechanisms in cardiac & skeletal muscle OBJECTIVES Methodology:Methodology: Chimeric proteinsChimeric proteins FRETFRET Differences in Ca."— Presentation transcript:

1 Ca 2+ release mechanisms in cardiac & skeletal muscle OBJECTIVES Methodology:Methodology: Chimeric proteinsChimeric proteins FRETFRET Differences in Ca 2+ release between cardiac and skeletal muscleDifferences in Ca 2+ release between cardiac and skeletal muscle

2 INTRODUCTION

3 Ca 2+ channel characteristics

4 Voltage sensitive Ca 2+ channel molecular structure

5 Mammalian Ca 2+ channel genes

6 SR Ca 2+ release channel – Ryanodine receptor (RyR)

7 RyR molecular structure

8 Mammalian ligand-gated Ca 2+ channel genes

9 DHPR-RyR molecular arrangment

10 Ca 2+ release in skeletal muscle

11 Ca 2+ release in cardiac muscle

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13 DISCUSSION

14 1.5 nA 15 nA 10 nA Cardiac sequence Skeletal muscle sequence pCac6 (Sk) pCARD1

15 pCac6 (Sk) pCARD1 Ca / Cd 2+ CELL SHORTENING Cardiac sequence Skeletal muscle sequence

16 I II III IV II-III LOOP

17 Serine 687 to alanine (S687A) Serine – PKA (only in Sk)

18 I II III IV II-III LOOP

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20 Dyspedic myotubes Region with RyR-2 cDNA injection Normal myotubes Dyspedic myotubes with RyR-2 Ca 2+ transient during spontaneous contractions

21 Normal myotubesDyspedic myotubes with RyR-2

22 Ca 2+ current RyR-2 expressing Dyspedic myotubes Ca 2+ current & [Ca 2+ ] i RyR-2 expressing Dyspedic myotubes

23 Ca 2+ current CSk3 expressing Dyspedic myotubes Ca 2+ transient Dyspedic myotubes Electrically evoked Spontaneous Failure (electrical)

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