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Rapid CE-UV Evaluation of Polymer-coated Magnetic Nanoparticles for Selective Binding of Endocrine Disrupting Compounds and Pharmaceuticals in Water by.

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Presentation on theme: "Rapid CE-UV Evaluation of Polymer-coated Magnetic Nanoparticles for Selective Binding of Endocrine Disrupting Compounds and Pharmaceuticals in Water by."— Presentation transcript:

1 Rapid CE-UV Evaluation of Polymer-coated Magnetic Nanoparticles for Selective Binding of Endocrine Disrupting Compounds and Pharmaceuticals in Water by Aromatic Interactions Prepared by Musharraf Miah Department of Chemistry Carleton University September 12,

2 Some Abbreviations 2 CE-UVCapillary electrophoresis with ultra-violet detector EDCsEndocrine disrupting compounds PPCPsPharmaceutical and personal care products BPABisphenol A MFMetformin PF Phenformin NAANaphthalene acetic acid TCTriclosan QS Quinine sulfate MNPsMagnetic nanoparticles Polypyrrole-coated magnetic nanoparticles magnetic nanoparticles FTIRFourier transform infrared spectroscopy SEM Scanning electron microscopy TGA Thermogravimetric analysis XRD X-ray diffraction

3 Outline  Introduction  Research objectives  Experimental  Results and Discussion  Conclusion  Future work  Acknowledgement 3

4 Introduction  EDCs alter the normal functions of the endocrine system in humans and animals  They mimic the body's own hormones and lead to negative health effects  BPA is widely used in plastics (toxic)  NAA is a plant hormone used in plant rooting horticultural products, pesticides on fruits and vegetables (also toxic)  TC is used in soaps, toothpastes, detergents, hand sanitizers, mouth and dish washes 4

5 Introduction(continue)  PPCPs- wide class of chemical contaminants originate from human usage and veterinary applications  Three PPCPs analyzed MF, PF, and QS  Long-term exposure to low levels of PPCP residues could have adverse effects on aquatic ecosystem and human health (Environment Canada)  EDCs and PPCPs are found in aquatic environment from sewage treatment plant effluent, agricultural runoff, concentrated animal feed, landfill leachates, and urban runoff 5

6 Introduction(continue)  MNPs are currently attracting a wide range of applications in water treatment (not target selective in complex water matrices )  Target selective MNPs 6

7 Chemical structures of target compounds 7 BPA MF NAA PF TC QS

8 Polypyrrole and polydopamine 8

9 Research objectives  Synthesize MNPs, and nanoparicles  Characterize the particles by FTIR, SEM, TGA and XRD  Evaluate these magnetic nanoparticles for selective binding with BPA, NAA, MF, PF, TC, and QS in water by CE-UV  Study the adsorption kinetics and adsorption isotherms of BPA, TC and PF  Utilize these particles as magnetic sorbents for the preconcentration of target compounds in water analysis 9

10 Experimental 10  Experimental setup for coating MNPs with PPy or PDA  Illustration of Fe 3 O 4 and Fe 3 O syntheses Ref: X. Wang, L. Wang, X. He, Y. Zhang and L. Chen, Talanta. 2009, 78, J. Meng, J. Bu, C. Deng and X. Zhang, J. Chromatogr. A., 2011, 1218, H. Z. Wen, H. L. Chun, C. G. Xiu, R. C. Fa, H. Y. Huang, and R. W. Xiao, J. Mater. Chem., 2010, 20,

11 FTIR spectra of MNPs, PPy and particles 11 Ref: T. Yao, T. Cui, J. Wu, Q. Chen, S. Lu, and K. Sun, Polymer chemistry, 2011, 2, 2893 Y. Wang, W. Chen, D. Zhou and G. Xue, Macromol. Chem. Phys., 2009, 210,

12 FTIR spectra of MNPs and particles 12 Ref: L. P. Zhu, J. H. Jiang, B. K. Zhu, and Y.Y. Xu, Colloid. Surface. B., 2011, 86,

13 SEM images of MNPs, 13  Average MNPs = nm, = nm, = nm  PPy coating = nm, PDA coating = nm

14 XRD spectra of MNPs and particles 14  Characteristic peaks for MNPs: 31.7°, 37.1°, 44.8°, 55.3°, 58.9° and 64.4°  New peaks for 14.6 ° and 22 ° Ref: A. Hrdina, E.P.C. Lai, C.S. Li, B. Sadi and G. Kramer, J. Magn. Magn. Mater., 2010, 322, T. Yao, T. Cui, J. Wu, Q. Chen, S. Lu, and K. Sun, Polymer chemistry, 2011, 2, 2893.

15 Capillary electrophoresis 15 Capillary Background electrolyte (BGE)

16 Results and Discussions 16 Electrophoretic mobility values of analytes, MNPs, and Mep = Mapp – Meo = [(Ld/ t) / (V/ Lt)] - [(Ld/ t neutral ) / V/Lt)] Migration Time (t in min) Molecular Weight (g.mol -1 ) Electrophoretic Mobility (m 2 V -1 s -1 ) Metformin (MF) 3.4 ± x10 -8 Phenformin (PF) 3.7 ± x10 -9 Quinine sulfate (QS) 4.0 ± x10 -9 Mesityl Oxide (MO) 4.2 ± Bisphenol A (BPA) 4.5 ± x10 -9 Triclosan (TC) 5.5 ± x10 -8 Naphthalene acetic acid (NAA) 6.9 ± x10 -8 MNPs 7.8 ± x ± x10 -8 ± x10 -8

17 CE-UV electropherogram for a mixture of BPA, MF, NAA, PF, TC, and QS (200 µg.mL -1 ) in 20 mM Na 2 HPO 4 BGE 17

18 In-capillary binding test results 18 Analytes (200 µg.mL - 1 ) % Binding with MNPs (10 mg. mL -1 ) % Binding with (10 mg. mL -1 ) % Binding with (10 mg. mL -1 ) Bisphenol A (BPA) 5 ± 365 ± 599 ± 1 Metformin (MF) 2 ± 214 ± 634 ± 4 Naphthalene acetic acid (NAA) 7 ± 321 ± 439 ± 6 Phenformin (PF) 5 ± 399 ± 1 Triclosan (TC) 6 ± 392 ± 399 ± 1 Quinine sulfate (QS) 8 ± 294 ± 298 ± 2

19 19 4 mAU detector signal 2 mAU detector signal Electropherograms of Standard BPA, PF, TC and NAA

20 In-capillary binding test 20 Electropherograms: Binding of BPA, PF, TC and NAA with

21 In-vitro binding test results 21 Analytes (200 µg. mL -1 ) % Binding with MNPs (9 mg. mL -1 ) % Binding with (9 mg.mL -1 ) % Binding with (9 mg.mL -1 ) Bisphenol A (BPA)10 ± 371 ± 594 ± 2 Metformin (MF)5 ± 243 ± 330 ± 4 Naphthalene acetic acid (NAA) 8 ± 222 ± 268 ± 4 Phenformin (PF)6 ± 394 ± 299 ± 1 Triclosan (TC)7 ± 395 ± 199 ± 1 Quinine sulfate (QS)10 ± 295 ± 199 ± 1

22 (A) 6 analytes mixture(B) Supernatant after extraction with MNPs (C) Supernatant after extraction with (D) Supernatant after extraction with Binding selectivity (in-vitro binding test) 19

23 Elution test of particles  A mixture of EtAc and MeOH (75:25, v/v) was selected as the eluting solvent  The % recoveries of the bound analytes were found to be 85±13% MF, 87±13% PF, 54±11% BPA, 52 ± 10% QS, 39 ±11% TC, and 37 ± 10% NAA 23 Ref: J. Meng, J. Bu, C. Deng and X. Zhang, J. Chromatogr. A., 2011, 1218,

24 Regeneration of particles 24  % Binding = 94±3%  % Recovery = 53±4%

25 Conclusion  High % binding (99 ± 1%) of with BPA, PF, TC, and QS were found due to π-π and hydrogen bonding interactions between PPy and analytes  Compared with unmodified MNPs, and showed higher binding efficiency towards aromatic compounds as confirmed by in- capillary and in-vitro binding tests.  Using EtAc and MeOH (75:25,v/v) as eluting solvent, the % recoveries of target compounds are found to be between 87% and 37% 25

26 Conclusion (continue)  The higher adsorption capacity (X m ) of particles was obtained for BPA, PF, and TC  Demonstrating strong affinity and better performance of particles as adsorbents for efficient removal of these target compounds  The new coating of PPy on the used particles proved to be time saving and cost effective in recycling the used particles 26

27 Future work 27  Investigate the binding interaction of these drugs with particles by CE-UV  The loaded particles can be used for specific drug delivery to target cancer cells Chemical structures of common anticancer drugs

28 Acknowledgements  I would like to express my deepest appreciation to my supervisor, Dr. Edward Lai for his supervision, advice and guidance  I am thankful to Dr. Zafar Iqbal for his kind assistance during this study  I am thankful to all colleagues in my group for sharing their experience and knowledge  I am thankful to Anita Chun and Dr. Wendy Hao for their technical assistance  Financial support from NSERC Canada is gratefully acknowledged. 28

29 29 Thank you for listening


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