S-Sepharose Design GE Chromaflow Column Currently max D = 1200mm, H = 30cm Custom Design available for D > 1200mm
Economics and Suppliers Unit ComponentUnit Cost S-Sepharose$1000/L Annual Report601.35 L/yr Total$601 350/yr Chromatography column$241 000 Other plant direct costs$488 000 First Year TOTAL$1330350 S-Sepharose available from GE Healthcare Chromaflow supplied by GE
IEC Alternatives SP-Sepharose: Sulphopropyl –O–CH 2 CHOHCH 2 OCH 2 CH 2 CH 2 SO 3 – S-Sepharose: Sulphoethyl no longer produced –O–CH 2 –CH 2 –SO 3 – Replaced by SP-Sepharose Anion exchange resin Q-sepharose DEAE HIC Columns SEC/Gel Filtration
IEC Alternatives DEAE: in accordance with German Patent 26 29 568 S-sepharose: in accordance with US Patent 5 101 013 ExchangerDEAE anion exchanger S-Sepharose cation exchanger MatrixVinyl polymerCrosslinked agarose gel Buffer, gradientpH 9, 0.1-0.25 M NaClpH 3.5, 0.15-0.35M NaCl Loading1 g/l3 g/l Yield60%80% Purity80-85%90-95% SeparationIncomplete separationBase line separation Comparison of IEC
No Questions Allowed Disclaimer: The views presented in this slide are not necessarily those of any or all team members.