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Transcriptional regulatory code of a eukaryotic genome Harbison CT et al. Nature, 2004, Vol. 431, pp. 99-104 Milica Volar 3 March 2005.

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Presentation on theme: "Transcriptional regulatory code of a eukaryotic genome Harbison CT et al. Nature, 2004, Vol. 431, pp. 99-104 Milica Volar 3 March 2005."— Presentation transcript:

1 Transcriptional regulatory code of a eukaryotic genome Harbison CT et al. Nature, 2004, Vol. 431, pp Milica Volar 3 March 2005.

2 Outline of talk Background Yeast’s transcriptional regulatory map Promoter structure Regulator binding behaviour Summary

3 Transcriptional regulators Proteins that bind to specific promoter sequences in genome Regulate genome expression and its maintenance Cell cycle, environment changes, replication, condensation, cohesion, etc.

4 Procedure

5 Genome wide location analysis Crosslink protein to DNA in vivo with formaldehyde Break open cells and shear DNA Immunoprecipitate Reverse-crosslinks, blunt DNA and ligate to unidirectional linkers LM-PCR Labeling Hybridize to array Ren B et al, 2004

6 Procedure They tested the binding by GWLA: 203 (84) DNA transcriptional regulators in rich media -> 11,000 unique interactions between regulators and promoter regions at high confidence (P<0.001)

7 Yeast transcriptional regulatory map

8 3,353 interactions within 1,296 promoter regions

9 Promoter architectures

10

11 Regulator binding behaviour

12

13 Summary Construction of the yeast transcriptional regulatory map Promoter architectures Patterns of regulator binding behaviour


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