Presentation on theme: "SPAGeDi a program for Spatial Pattern Analysis of Genetic Diversity by Olivier J. Hardy and Xavier Vekemans SPAGeDi a program for Spatial Pattern Analysis."— Presentation transcript:
SPAGeDi a program for Spatial Pattern Analysis of Genetic Diversity by Olivier J. Hardy and Xavier Vekemans SPAGeDi a program for Spatial Pattern Analysis of Genetic Diversity by Olivier J. Hardy and Xavier Vekemans Goal: characterise spatial genetic structure of mapped individuals or populations using genotype data of any ploidy level Compute: - inbreeding coef - pairwise relatedness/differentiation coef between indiv/pop averages / distance classes association with distance (regression with lin/log distance) ( isolation by distance, neighbourhood size estimates) - actual variance of relatedness coef Ritland’s approach for marker based estimate of h 2 Tests: - permutations (of genes, individuals, or spatial locations) - jackknife over loci ( SE for multilocus estimates) Option: - restricted analysis within or among categories of ind/pop
Input data Input file with : - format #’s (#ind, #categ, #spat coord, #loci, #digits/allele, ploidy) - distance intervals - for each ind : - name - category (facultative) - spatial coordinates - genotype at each locus Analyses defined on keyboard while running the program : - indiv vs pop level - stat to compute (+ within/among categ) - tests, …
2-genes coef : - "kinship" coef (Loiselle 1995; Ritland 1996) - "relationship" coef (Moran’s I; Lynch & Ritland 1999; Wang 2002) - kinship type coef based on allele size (Streiff et al. 1999) - a r distance measure (Rousset 2000) 4-genes coef : - "fraternity" coef (Lynch & Ritland 1999; Wang 2002) Statistics computed: "relatedness" coef at the individual level also for dominant marker (Hardy 2003)
Estimates of the actual variance of pairwise kinship coef in natural populations
Consistency among kinship coef estimators
Reliable estimates of the actual variance of pairwise relatedness require - large data set (300 – 1000 individuals) - very polymorphic markers and/or many loci SSR AFLP ???