Presentation on theme: "Honors Biology Chapter 1 Section 4 SC B-1: Students will demonstrate an understanding of how scientific inquiry & technological design can be used to pose."— Presentation transcript:
Honors Biology Chapter 1 Section 4 SC B-1: Students will demonstrate an understanding of how scientific inquiry & technological design can be used to pose questions, seek answers, & develop solutions
Essential Question: How would you compare & contrast a light microscope & an electron microscope?
Metric System used by most scientists based on certain physical standards scaled on multiples of 10 used in SI
Common Metric Units length 1 meter (m) = 100 centimeters(cm) 1 meter (m) = 1000 millimeters (mm) 1000 meters(m) = 1 kilometer (km) mass 1 kilogram (kg) = 1000 grams (g) 1 gram (g) = 1000 milligrams (mg) 1000 kilograms (kg) = 1 metric ton (t)
more metric units volume 1liter (L) = 1000milliliters( mL) 1 liter (L) = 1000 cubic centimeters (cm3) temperature 0◦ C = freezing point of water 100◦ C = boiling point of water
Analyzing Biological Data Data Tables create a table to record information measured in an experiment name the table
Biological Data new field of BIOINFORMATICS Computers used: To store data To make sense of data To collect da ta
Microscopes devices that produce magnified images of structures too small to see with naked eye
Light Microscopes produce images by focusing visible light rays through a specimen limitations < 0.2 µ
Electron Microscope produce magnified images by focusing beams of electrons on specimen
Compound Light Microscope up to 1000x magnification light passes through the specimen uses 2 lenses specimen dead or alive (if very small) specimen may be stained to improve visulaization Stain entire cell Stain selected organelles or macromolecules
Parts of a Microscope Appendix D: Parts& Functions
Electron Microscopes Can produce images 1000 times more detailed than light microscope
Types of Electron Microscopes TEMs (Transmission Electron Microscope) beam of e- goes thru specimen SEMs (Scanning Electron Microscopes) beam scan across surface of specimen
comparing Microscopes Electron Compound Light cannot use living cells Very expensive higher resolution Specimen could be living Price not an issue Size limitations
Cell Cultures Take small sample of cells & place in petri dish with nutrients & allowed to divide.
Cell Cultures might use the culture to: 1. test cell responses under controlled conditions 2. study interactions between cells 3. select certain cells for further study
HeLa Cells Taken from a patient with cervical cancer in 1950’s Cells are still growing!
Bacterial Cultures Made whenever something has been tested for infection
Cell Fractionation Separates cells into parts
Lab Safety Rules Always follow read through lab completely before starting Always follow instructions Ask if you do not understand directions Always wash your hands at end of lab