1. Little Calumet River Near O’Brien Lock and Dam Rotenone sample area = 2.6 miles of channel or 150 surface acres of water Eleven nets (total = 16,800 feet) set May 20, run and reset May 21 and 22, and pulled May 23 Water samples for eDNA analysis collected morning of May 20 DC electrofishing for sport fish for a total of 20 hours on May 12 No Asian carp captured or seen No Asian carp DNA detected

3. JA – 01 Trammel and gill net locations for sampling conducted May 20 – May 23, 2010 by commercial fishermen Ron Brown and John Ahrling. Nets were 300-600 feet long x 5-12 ft. deep with bar mesh sizes ranging from 2.5 -3.5 inches. RB - 01 JA – 02 JA – 03 JA – 04 RB - 02 RB - 03 RB - 04 RB - 05 RB - 06 RB - 07

4. Rotenone Fish Recovery Summary Number of individuals67,224 Number of species38 Hybrid groups2 Total weight recovered (pounds)97,720 Standing stock (lbs./acre)565

5. Commercial Net Sample Totals SpeciesNumberPercent Common carp561 59.3 Freshwater drum174 18.4 Gizzard shad148 15.6 Channel catfish33 3.5 Black buffalo16 1.7 Goldfish5 0.5 Carp x Goldfish hybrid5 0.5 Yellow bullhead2 0.2 Yellow bass1 0.1 White perch1 0.1

6. SpeciesNumberPercent Alewife71.1 Black buffalo20.3 Black bullhead10.2 Bluegill111.7 Bluntnose minnow447 Channel catfish172.7 Common carp21634.1 Emerald shiner619.6 Fathead minnow91.4 Freshwater drum50.8 Gizzard shad7612 Golden shiner10.2 Goldfish447 Green sunfish30.5 SpeciesNumberPercent Rainbow trout10.2 Rock bass30.5 Round goby467.3 Weather loach10.2 White bass20.3 White crappie10.2 White perch243.8 White sucker71.1 Yellow bass20.3 Yellow bullhead20.3 Yellow perch40.6 Total number633100 Electrofishing Sample Totals

7. SpeciesNumberPercent Gizzard shad2229833.2 Common carp982014.6 Ghost shiner694510.3 Emerald shiner43426.5 Alewife34855.2 Freshwater drum31784.7 Bluntnose minnow23743.5 Goldfish22933.4 Round goby21093.1 Channel catfish19592.9 Rock bass12441.8 Pumpkinseed10981.6 White perch10591.6 Bluegill7831.2 Spotfin shiner4640.7 Golden shiner4340.6 Yellow bullhead3660.5 Black crappie3330.5 Fathead minnow3250.5 White crappie3190.5 SpeciesNumberPercent White sucker3190.5 Yellow perch2680.4 Black bullhead2640.4 Largemouth bass2190.3 Spottail shiner2130.3 Black buffalo2050.3 Orangespotted sunfish1740.3 White bass820.1 Goldfish x Common carp hybrid530.1 Green sunfish490.1 Smallmouth bass450.1 Grass carp430.1 Warmouth18<0.1 Smallmouth buffalo12<0.1 Yellow bass10<0.1 Brown bullhead80.0 Flathead catfish8<0.1 Grass pickerel2<0.1 Hybrid sunfish2<0.1 Rotenone Fish Recovery Species List