1. Considerations for Flow Measurement on the Mouse Femoral Artery
Location of Measurement with the Flowprobe
Vessel Diameter
Maintaining Body Temperature
Experimental Set-up
Photos and data courtesy of Michael F. Callahan
Depts. of Orthopaedic Surgery,
Physiology & Pharmacology,
Wake Forest University Health Sciences
Winston-Salem, North Carolina
All information in this file is privileged information and may
not be used without permission of the author.

2. Branches of Femoral Artery Before and After Dissection
Epigastric Nerve,
Artery and Vein
Approximate Location of Knee
Femoral Nerve,
Artery and Vein exiting from abdominal wall
Femoral Nerve, Superficial Femoral Artery and Vein
Transonic
Systems Inc.

3. Femoral artery proximal to the epigastric a
Femoral artery proximal to the epigastric a., isolated from the femoral vein, measures ~ 400 µm diameter with a micrometer. Flow measurements can be made in this location easily.
Transonic
Systems Inc.

4. Femoral Blood Flow in Anesthetized Mouse Recorded with Transonic Systems’ Flowmeter & Nanoprobe
Systems Inc.
Data Courtesy of Michael F. Callahan, Wake Forest Health Sciences, Winston-Salem, NC
MFCallahan, 3/03

5. Effect of Core Temperature on Femoral Blood Flow (22g CD-1, 3-16-03)
0.82 ml/m
37°C
0.64 ml/m
35°C
0.51 ml/m
33°C
0.27 ml/m
Temperature has a profound effect on femoral blood flow and must be monitored.
Data Courtesy of Michael F. Callahan, Wake Forest Health Sciences, Winston-Salem, NC

6. Flow measurements on the femoral artery distal to the epigastric a.
Isolation of the artery from the vein in this position is very challenging. There is less fascia and connective tissue around the sheath of the artery and vein to manipulate the vessel free.
The nerve runs immediately adjacent and is very easy to tear.
Once isolated, avoid pulling up on the artery which would cause vascular spasm or putting pressure on the vein to cause occlusion.
Superficial femoral a.

7. The superficial femoral artery, distal to the epigastric a
The superficial femoral artery, distal to the epigastric a., is isolated from the femoral vein and measures ~ 200 µm diameter on the scale.

8. Transonic 0.5PSB flowprobe on the superficial femoral artery distal to the epigastric a.

9. Sample flow trace from lower on femoral artery near the knee
Sample flow trace from lower on femoral artery near the knee. Since the vessel diameter fills < 60% of the probe lumen, probe position must remain fixed for a stable measurement.
32g male CD-1 with 0.5PSB probe
on superficial femoral artery
Temperature 33o C.
Michael Callahan, Wake Forest Univ. Health Sciences, 2/5/2004

10. Experimental Setup for Femoral Artery Blood Flow Study
Close up of the Transonic nanoprobe handle clamped in a micromanipulator with two axis adjustment to fine tune and stabilize position of probe for measurement.

11. Experimental Setup for Femoral Artery Blood Flow Study
TransonicSystems 400-Series Flowmeter Console
Probe holder with magnetic base, adjustable arm, and micro-positioning adjustment
Heating pad & lamp used with gauze cover to maintain mouse core temperature. Temp. is monitored with an anal probe.

12. Simultaneous Femoral Artery Blood Flow & Pressure Recording

13. Flow recorded from the superficial femoral a
Flow recorded from the superficial femoral a. with occlusion; Pressure recorded from the contra lateral femoral a.

14. Photos and data courtesy of :
Transonic Systems, Inc. gratefully acknowledges the investigators for the presentation of this data on the Transonic website. All information in this file is privileged information and may not be used without permission of the authors.
Photos and data courtesy of :
Michael F. Callahan, PhD
Thomas L. Smith, PhD
Depts. of Orthopaedic Surgery,
Physiology & Pharmacology,
Wake Forest University Health Sciences
Winston-Salem, North Carolina
Edited by Margo Sosa, Sr. Product Manager
Transonic Systems, Inc.
December 2004