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The innate immune system as a window into sponge-microbe interactions Jo Bayes Sandie Degnan School of Biological Sciences UQ.

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Presentation on theme: "The innate immune system as a window into sponge-microbe interactions Jo Bayes Sandie Degnan School of Biological Sciences UQ."— Presentation transcript:

1 The innate immune system as a window into sponge-microbe interactions Jo Bayes Sandie Degnan School of Biological Sciences UQ

2 Questions 1.How does Amphimedon acquire its symbionts? 2.How does the bacterial community of Amphimedon change throughout its lifecycle? 3.How does innate immune gene expression change throughout Amphimedon’s lifecycle?

3 Ancient, abundant, invisible life forms

4 Microbial-metazoan interactions Marine bacteria - freeliving - pathogenic - symbiotic Bacteria 3.5 bya Sponges 650 mya

5 Amphimedon queenslandica Heron Island Indo-Pacific distribution Hermaphroditic spermcast spawner Broods embryos First Poriferan genome Srivastava et al. 2010 Alpha - proteobacteria bacteria sequenced

6 Questions 1.How does Amphimedon acquire its symbionts? 2.How does the bacterial community of Amphimedon change throughout its lifecycle? 3.How does innate immune gene expression change throughout Amphimedon’s lifecycle?

7 Microbial -metazoan interactions Symbiont transmission Vertical - Poribacteria Horizontal

8 Questions 1.How does Amphimedon acquire its symbionts? 2.How does the bacterial community of Amphimedon change throughout its lifecycle? 3.How does innate immune gene expression change throughout Amphimedon’s lifecycle?

9 Microbial communities PC larvaePost larvaeAdultFSW Adult Shark Bay Seawater PC larvae Post larvae Adult FSW Adult Least similar Most similar

10 Questions 1.How does Amphimedon acquire its symbionts? 2.How does the bacterial community of Amphimedon change throughout its lifecycle? 3.How does innate immune gene expression change throughout Amphimedon’s lifecycle?

11 Innate Immunity: A first line recognition system Vertebrates Innate and Adaptive Memory cells Invertebrates Innate Alternative adaptive ?

12 Innate Immunity: A first line recognition system P attern R ecognition R eceptors M icrobial A ssociated M olecular P atterns Gram positiveGram negative

13 Gene familyMAMPs detectedGene(s) in Amphimedon Homologues VertebratesInvertebrates LBLLPS: G –ve bacteria3 NLRPGN: G+ve and -ve2 C-lectinCarbohydrates1 Amphimedon innate immune genes of interest

14 Amphimedon innate immune gene expression

15 Conclusions Symbiotic microbes acquired via vertical and horizontal transmission Symbiotic from transitory community Immune genes induced or developmental? Benthos Feeding Thank you... Sandie Degnan Bernie Degnan (School of Biological Sciences, UQ) Phil Hugenholtz (Australian Centre of Ecogenomics) Degnan lab http://web.me.com/degnanlab1/DegnanLab/H ome.html http://ecogenomic.org/users/jo-bayes


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