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Picture Hamilton Venus Software Intelligent Liquid Sensing.

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Presentation on theme: "Picture Hamilton Venus Software Intelligent Liquid Sensing."— Presentation transcript:

1 Picture Hamilton Venus Software Intelligent Liquid Sensing

2 2 Intelligent Liquid Sensing 1. Capacitive Liquid Level Detection (cLLD) 2. Pressure Liquid Level Detection (pLLD) 3. Anti Droplet Control (ADC) 4. Capacitance Clot Detection 5. Monitored Air Displacement (MAD) 6. Total Aspiration and Dispense Monitoring (TADM)

3 3 Intelligent Liquid Sensing Conditions:  Black tips or needles  Conductive liquids only  Clean or used tips  All channels  96 head - A1 and H12 only  384 Head – A5 and P24 only  Aspirate and or dispense steps Capacitance Liquid Level Detection More Details

4 4  Set submerge depth (mm down from detected liquid)  Select cLLD  Click Customize for more Intelligent Liquid Sensing  Set submerge depth (mm down from detected liquid) cLLD in detail  Select cLLD  Click Customize for more

5 5  Set sensitivity Intelligent Liquid Sensing  Set sensitivity cLLD in detail  Set submerge depth (mm down from detected liquid)  Select cLLD  Click Customize for more

6 6 Intelligent Liquid Sensing 1. Capacitive Liquid Level Detection (cLLD) 2. Pressure Liquid Level Detection (pLLD) 3. Anti Droplet Control (ADC) 4. Capacitance Clot Detection 5. Monitored Air Displacement (MAD) 6. Total Aspiration and Dispense Monitoring (TADM)

7 7 Intelligent Liquid Sensing Pressure Liquid Level Detection More Details Conditions:  Black / clear tips  Any liquid  Clean tips, aspirate only  All channels

8 8  Select pLLD  Click Customize for more  Set submerge depth (mm down from detected liquid)) Intelligent Liquid Sensing  Select pLLD pLLD in detail  Click Customize for more  Set submerge depth (mm down from detected liquid))

9 9  Set sensitivity Intelligent Liquid Sensing  Set sensitivity pLLD in detail  Select pLLD  Click Customize for more  Set submerge depth (mm down from detected liquid))

10 10 Intelligent Liquid Sensing When to use cLLD & pLLD Bubbles on liquid surface  Use pLLD to find the liquid surface  cLLD could be triggered by the bubbles Aqueous layer under organic layer  Use pLLD to find the non- conductive organic layer  Use cLLD to find the conductive aqueous layer

11 11 Intelligent Liquid Sensing 1. Capacitive Liquid Level Detection (cLLD) 2. Pressure Liquid Level Detection (pLLD) 3. Anti Droplet Control (ADC) 4. Capacitance Clot Detection 5. Monitored Air Displacement (MAD) 6. Total Aspiration and Dispense Monitoring (TADM)

12 12 Intelligent Liquid Sensing With ADC – the pressure increase inside the channel is measured and the piston moved to compensate. Anti-Droplet Control Without ADC – high vapour pressure of volatile liquid results in a pressure increase in the tip forcing the liquid out. Plunger position

13 13 Intelligent Liquid Sensing Prevent droplets on tips when pipetting highly volatile liquids This is a standard feature in VENUS Anti-Droplet Control Conditions:  Black / clear tips or needles  Channels only  Pre-wetting tips improves performance More Details

14 14  Turn ADC off immediately after the dispense step  Turn ADC on immediately after the aspirate step  Turn ADC off in the OnAbort submethod too Intelligent Liquid Sensing  Turn ADC off immediately after the dispense step ADC in detail  Turn ADC on immediately after the aspirate step  Add HSLML_STARLib to method  Turn ADC off in the OnAbort submethod too

15 15  Click customise to change advanced settings Intelligent Liquid Sensing ADC in detail If the initial pressure rise is very high, ADC will error. Pre-wetting the tip allows the pressure to reach equilibrium before the aspirate  Pre-wet the tip using a mix on the aspirate step  Click customise to change advanced settings  Click Error Settings to set reaction to ADC error

16 16  Pre-wet the tip using a mix on the aspirate step Intelligent Liquid Sensing ADC in detail If the initial pressure rise is very high, ADC will error. Pre-wet the tip to allow the pressure to reach equilibrium before the aspirate  Pre-wet the tip using a mix on the aspirate step  Click customise to change advanced settings  Click Error Settings to set reaction to ADC error

17 17  Click Error Settings to set reaction to ADC error Intelligent Liquid Sensing ADC in detail If the initial pressure rise is very high, ADC will error. Pre-wet the tip to allow the pressure to reach equilibrium before the aspirate  Pre-wet the tip using a mix on the aspirate step  Click customise to change advanced settings  Click Error Settings to set reaction to ADC error

18 18 Intelligent Liquid Sensing 1. Capacitive Liquid Level Detection (cLLD) 2. Pressure Liquid Level Detection (pLLD) 3. Anti Droplet Control (ADC) 4. Capacitance Clot Detection 5. Monitored Air Displacement (MAD) 6. Total Aspiration and Dispense Monitoring (TADM)

19 19 Intelligent Liquid Sensing The conductive signal after aspiration is measured.  gap between tip and liquid - the capacitance signal will vanish.  clot - it bridges the gap and the signal remains, resulting in an error message Capacitance Clot Detection More Details Conditions:  Black tips or needles  Conductive liquids only  Clean or used tips  All channels

20 20  Turn clot detection monitoring on  Turn clot detection monitoring off after aspirate  Turn clot detection monitoring off in the OnAbort submethod too Intelligent Liquid Sensing  Turn clot detection monitoring on Capacitance Clot Detection in detail  Turn clot detection monitoring off after aspirate  Add HSLML_STARLib to method  Turn clot detection monitoring off in the OnAbort submethod too

21 21  Works only with cLLD  Click Error Settings to set reaction on error Intelligent Liquid Sensing Capacitance clot detection in detail  Works only with cLLD  Click Error Settings to set reaction on error

22 22  The clot retract height is set in the liquid class Intelligent Liquid Sensing  The clot retract height is set in the liquid class Capacitance Clot Detection in detail

23 23 Intelligent Liquid Sensing 1. Capacitive Liquid Level Detection (cLLD) 2. Pressure Liquid Level Detection (pLLD) 3. Anti Droplet Control (ADC) 4. Capacitance Clot Detection 5. Monitored Air Displacement (MAD) 6. Total Aspiration and Dispense Monitoring (TADM)

24 24 Intelligent Liquid Sensing Monitored Air Displacement Tip Blocked Pressure Time Correct Aspiration Pressure Threshold Empty tip Tip filling with liquid under vacuum Tip filled with correct volume of liquid Tip in liquid start aspirating Tip blocked Plunger stops moving, tip continues to fill

25 25 Intelligent Liquid Sensing Monitored Air Displacement Conditions:  Black / clear tips  Clean tips, aspirate only  All channels  Lower limit varies but >10µl  MAD has only been optimised for aqueous liquids MAD can be switched on for individual aspiration steps in a method The pressure threshold can be varied to detect whether each pipetting step was successful More Details

26 26  Turn aspiration monitoring off in the OnAbort submethod too  Turn aspiration monitoring on  Set pressure threshold  Turn aspiration monitoring off after aspirate Intelligent Liquid Sensing  Turn aspiration monitoring on MAD in detail  Set pressure threshold  Turn aspiration monitoring off after aspirate  Add HSLML_STARLib to method  Turn aspiration monitoring off in the OnAbort submethod too

27 27  Works with or without pLLD  Click Error Settings to set reaction if pressure threshold is crossed Intelligent Liquid Sensing MAD in detail  Works with or without pLLD  Click Error Settings to set reaction if pressure threshold is crossed

28 28 Intelligent Liquid Sensing 1. Capacitive Liquid Level Detection (cLLD) 2. Pressure Liquid Level Detection (pLLD) 3. Anti Droplet Control (ADC) 4. Capacitance Clot Detection 5. Monitored Air Displacement (MAD) 6. Total Aspiration and Dispense Monitoring (TADM)

29 29 Intelligent Liquid Sensing Total Aspiration and Dispense Monitoring TADM increases the safety and robustness of pipetting processes by recording and monitoring pressure throughout the pipetting cycle. It’s main use in in clinical settings where a permanent record showing correct pipetting of every sample is needed. Conditions:  Black / clear tips  Clean tips  All channels  96 TADM head  Requires additional TADM software

30 30 Intelligent Liquid Sensing Total Aspiration and Dispense Monitoring Various pipetting errors change the pressure curves in interpretable ways Aspirate Dispense

31 31 Intelligent Liquid Sensing AspirateDispense Total Aspiration and Dispense Monitoring  The user sets both TADM tolerance bands and the required response if they are crossed  Bands are set, using test data, for every liquid, tip type and volume used


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