1. halosperm® How to Interpret the Results Training Slides
Halotech DNA

2. How to Calculate Sperm DNA Fragmentation Index
After sample processing with Halosperm®, stain the slide with DIFF-QUICK®/Panoptic stain
Check the sample with a bright field microscope, and classify sperm cells as follows:

3. How to use the following training slides
Use the first 5 slides to learn how to classify each sperm cell.
Use the last 3 slides to practice calculating sperm DNA fragmentation on your own.

4. Example 1 Normal= 10 Fragmented= 4 SDF= 28.5% fragmented normal normal

5. Example 2 Normal= 17 Fragmented= 6 SDF= 26.1% normal normal fragmented

6. Example 3 Normal= 19 Fragmented= 1 SDF= 5% normal normal normal normal

7. Example 4 Normal= 15 Fragmented= 3 SDF= 16.6% normal normal normal

8. Example 5 Normal= 10 Fragmented= 3 SDF= 23.1% normal normal normal

9. Normal= 16
Fragmented= 7
SDF=30.4%

10. Normal= 21
Fragmented= 6
Degraded=1
SDF= 25%

11. Normal= 10
Fragmented= 3
SDF= 23.1%