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Technical Seminar-2004 Presented by :- Pramoda Padhy (EI200117092) Under the guidance of Mr. Vivek sharma Presented By Pramoda Padhy Roll no-(EI200117092)

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Presentation on theme: "Technical Seminar-2004 Presented by :- Pramoda Padhy (EI200117092) Under the guidance of Mr. Vivek sharma Presented By Pramoda Padhy Roll no-(EI200117092)"— Presentation transcript:

1 Technical Seminar-2004 Presented by :- Pramoda Padhy (EI ) Under the guidance of Mr. Vivek sharma Presented By Pramoda Padhy Roll no-(EI ) NATIONAL INSTITUTE OF SCIENCE & TECHNOLOGY Palur Hills,Berhampur ,Orissa,India BIOSENSORS

2 Technical Seminar-2004 Presented by :- Pramoda Padhy (EI ) INTRODUCTION What Is a Biosensor? Biosensor = bioreceptor + transducer. The bioreceptor is a biomolecule that recognizes the target analyte whereas the transducer converts the recognition event into a measurable signal. Enzyme is a Bioreceptor Requirements for Sensors

3 Technical Seminar-2004 Presented by :- Pramoda Padhy (EI ) Need for Biosensor Diagnostic Market The current climate of prevention the need for detection at increasingly lower limits is increasing in many diverse areas Clinical Testing clinical testing is one of the biggest diagnostic markets clinical testing products market in excess of 4000 million US$ in the 1990s Other Markets The medical arena (Technical Insights Inc.) with veterinary and agricultural applications

4 Technical Seminar-2004 Presented by :- Pramoda Padhy (EI ) Specificity With biosensors, it is possible to measure specific analytes with great accuracy. Speed analyte tracers or catalytic products can be directly and instantaneously measured Simplicity receptor and transducer are integrated into one single sensor& the measurement of target analytes without using reagents is possible Continuous monitoring capability Biosensors regenerate and reuse the immobilized biological recognition element

5 Technical Seminar-2004 Presented by :- Pramoda Padhy (EI ) Applications of Biosensor Health Care –Measurement of Metabolites –Market Potential –Diabetes –Insulin Therapy –Artificial Pancreas Industrial Process Control –Bioreactor Control Military Application Environmental Monitoring –Air and Water Monitoring

6 Technical Seminar-2004 Presented by :- Pramoda Padhy (EI ) Bioreceptor Molecules Enzymes Enzymes act as catalysts for biochemical reactions occurring in the cell Antibody Antibodies constitute about 20% of the total plasma protein and are collectively called immunoglobulin Receptor Protein Receptor proteins having specific affinity for hormones, antibodies, enzymes Other Possibilities

7 Technical Seminar-2004 Presented by :- Pramoda Padhy (EI ) Transducers Conventional Transducers Piezoelectric Transducers Coniductimetry Capacitance Measurement Thermometry Enzyme Thermistor FET as a Transducer

8 Technical Seminar-2004 Presented by :- Pramoda Padhy (EI ) Growth of Biosensor Biosensor Configurations Discriminative Membrane Sensitivity Requirements Immunoassay DNA Probe Evolution of Biosensors

9 Technical Seminar-2004 Presented by :- Pramoda Padhy (EI ) Receptors Receptors at Membrane Biological receptors are protein molecules most of them are bound to the cell membrane Hormone Receptors Many hormones released into the blood do not penetrate the cell membrane but react with specific receptors at the cell surface Smell Receptor taste and olfactory receptors are typical examples of this biospecific recognition process Light Receptor

10 Technical Seminar-2004 Presented by :- Pramoda Padhy (EI ) Types of biosensor Glucose Biosensor Amperometric Biosensor Glucose biosensor using glucose oxidase is used as an example Potentiometer Biosensor Examples of Potentiometric Biosensor Penicillin biosensor Glucose biosensor Urea biosensor

11 Technical Seminar-2004 Presented by :- Pramoda Padhy (EI ) Design Variables Immobilization Methods (1)adsorption (2) entrapment (3) covalent coupling (4) cross-linking Change in Conformation Change in Microenvironment Non-Uniform Distribution Reaction and Diffusion

12 Technical Seminar-2004 Presented by :- Pramoda Padhy (EI ) Future Prospects Data Processing and Pattern Recognition Micro Instrument Molecular Electronics Multi-Disciplinary Nature

13 Technical Seminar-2004 Presented by :- Pramoda Padhy (EI ) CONCLUSION Biosensors consist of bio-recognition systems, typically enzymes or binding proteins, such as antibodies, immobilized onto the surface of physico-chemical transducers Disadvantages They cannot be steam sterilized They react with the product And are oversensitive

14 Technical Seminar-2004 Presented by :- Pramoda Padhy (EI )


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