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Curcumin Induces both Apoptosis and Autophagy in Human Papillary Thyroid Carcinoma BCPAP Cells Zhang Li Jiangsu Institute of Nuclear Medicine Wuxi, Jiangsu,

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Presentation on theme: "Curcumin Induces both Apoptosis and Autophagy in Human Papillary Thyroid Carcinoma BCPAP Cells Zhang Li Jiangsu Institute of Nuclear Medicine Wuxi, Jiangsu,"— Presentation transcript:

1 Curcumin Induces both Apoptosis and Autophagy in Human Papillary Thyroid Carcinoma BCPAP Cells Zhang Li Jiangsu Institute of Nuclear Medicine Wuxi, Jiangsu, China Contact: 10 th AOTA Congress BALI, INDONESIA, 2012

2 Curcumin An acitve polyphenol derived from the plant turmeric low toxicity anti-inflammatory antioxidant antiangiogenic antitumour Chemical structure of curcumin CurcuminThyroid cancer ?

3 Apoptosis and Autophagy Apoptosis –type I programmed cell death Autophagy- type II programmed cell death - a self-degradative process - cope with stresses - required in apoptotic-deficient cells Three types of autophgay: -Microautophagy -Chaperone-mediated autophagy -Macroautopahgy

4 Part I Crucumin suppresses BCPAP cells growth

5 AB Effects of curcumin on cell viability

6 Effects of curcumin on cell morphology

7 PI/Hoechst staining A B

8 Part II Curcumin induces apoptosis in BCPAP cells

9 Effects of curcumin on pro-apoptotic and anti-apoptotic proteins A B

10 Effects of curcumin on loss of mitochondrial membrane potential (MMP)

11 Caspase-dependent apoptosis in curcumin-treated cells

12 Part III Curcumin induces autophagy in BCPAP cells

13 Induction of autophagy in curcumin-treated cells A B The FASEB JournalJune 2003vol. 17 no

14 RFP-LC3 Curcumin (  M) 0 50 C Induction of autophagy in curcumin-treated cells

15 Effects of curcumin on the phosphorylation of AKT pathway A B

16 Effects of curcumin on the phosphorylation of MAP kinases

17 Curcumin selectively induces autopagy in tumor cells A B

18 Curcumin seletively induces cancer cell death AB

19 Dynamic distribution of curcumin in BCPAP cells Enlarged 6 h 12 h 24 h

20 Effects of curcumin on DNA conformation A B

21 Conclusions  Curcumin induces cytotoxicity of BCPAP cells.  Induction of apoptosis and autophagy as the likely underlying mechanism that is responsible for the cytotoxicity of curcumin.  Curcumin inhibited AKT pathway and activated mitogen- activated protein kinase (MAPK) signaling thereby inducing autophagy.

22 Bao Jiandong Yu Huixin Zhang Chiyu Wu Jing Zheng Jie Sun Wei China Medical University Guan Haixia Nangjing University Sun Yang Acknowledgements Jiangsu Institute of Nuclear Medicine Grants  National Natural Science Foundation of China (No )  Natural Science Foundation of Jiangsu Province (No. BK )  Science and Research Foundation of Health Bureau of Jiangsu Province (No. H )


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