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Copyright 2006 by BioTX Automation, Inc. WellAware Forensic Package for use with…

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Presentation on theme: "Copyright 2006 by BioTX Automation, Inc. WellAware Forensic Package for use with…"— Presentation transcript:

1 Copyright 2006 by BioTX Automation, Inc. WellAware Forensic Package for use with…

2 Copyright 2006 by BioTX Automation, Inc. Settings Menu Used to allow customization for any lab.

3 Copyright 2006 by BioTX Automation, Inc. WellAware Forensic Package Flow Diagram WellAwareComplete ABI 310/3100 Sequencer WellAwareCompact ABI 7000 RT-PCR

4 Copyright 2006 by BioTX Automation, Inc. WellAware Forensic Package 1.Samples that have been extracted and are ready for quantification with an AB 7000 Real Time PCR instrument, are entered into the plate form and then printed. 2.The next step is to export a file for the quantification setup in WellAware format. This file is opened in WellAware which then guides the scientist to load the extracted samples into the AB 7000 Real Time PCR plate. WellAware voice and light cues direct the addition of master mix to the wells. 3.In the Forensic Interface, the scientist exports plate format information for running the AB Once the AB 7000 real time PCR instrument has completed the quantification of samples, the results from the AB 7000 Prism Instrument software are imported back into the Forensic Interface. WellAware Forensic Package How it Works WellAwareComplete ABI 7000 RT-PCR WellAware Forensic Package Plate Form for ABI

5 Copyright 2006 by BioTX Automation, Inc. WellAware Forensic Package 5.The DNA processing screen allows the scientist to add the samples that have now been quantitated to an AB 310 or AB 3100 plate for electrophoresis and detection. This set up is flexible, and the scientist can run a sample multiple times, and can even override the dilution amount if they choose. Additional sample descriptions, for example case number, can be added under the sample header. 6.Once the scientist has the samples in the order they have specified, they can just click on either the AB 310 or AB 3100 tab, depending upon which platform they would like to set up. After printing the sample set-up sheet, a WellAware file can be created by clicking on the Export Data tab and designating whether to pipette by rows or columns. 7.The exported file is opened in the WellAware software and the scientist can now set up their dilution plate for amplification. WellAware calls out how much dilutant (TE or H2O) to add to each well of the dilution plate. It will then ask for the barcoded extracts to be scanned. Once scanned, WellAware calls out the volume of DNA to add to each well of the dilution plate, and lights up the destination well. Once the sample has been pipetted the footswitch is used to proceed to the next sample. 8.The final step in using the Forensic Interface is in the exporting of the AB 310 or AB 3100 file. The information put into the processing screen for the dilution, step 6, is used to generate the file for the AB 310 or AB 3100 Genetic Analyzers. WellAware Forensic Package How it Works ABI 310/3100 Sequencer WellAwareCompact Processing Screen Norm & 310/3100 data

6 Copyright 2006 by BioTX Automation, Inc. WellAware Forensic Package Main Screen Step 1 Step 6 Step 5 Step 2 Step 7 Step 8 Step 4 Step 3

7 Copyright 2006 by BioTX Automation, Inc. Quant Worksheet Step 1

8 Copyright 2006 by BioTX Automation, Inc. WellAware Quant RT-PCR Pipetting Data (Page 1) Step 2

9 Copyright 2006 by BioTX Automation, Inc. WellAware Quant RT-PCR Pipetting Data (Page 2)

10 Copyright 2006 by BioTX Automation, Inc. WellAware Quant Pipetting a b c Step 3

11 Copyright 2006 by BioTX Automation, Inc. ABI 7000 real-time PCR Export Data Step 4

12 Copyright 2006 by BioTX Automation, Inc. ABI 7000 RT-PCR system Normalization Data Raw data from ABI 7000 Calculated normalization values Step 6

13 Copyright 2006 by BioTX Automation, Inc. Normalization Process Step 5 Step 6 Pull down menu Step 7

14 Copyright 2006 by BioTX Automation, Inc. WellAware Normalization Pipetting Data Step 7

15 Copyright 2006 by BioTX Automation, Inc. ABI 310/3100 (Genetic Analyzer) Worksheet Step 7 Step 8

16 Copyright 2006 by BioTX Automation, Inc. Contract Engineering and Software Utilizes companys core technology strengths Software and engineering Customers: MWG, PicoScript, Valco, Sigma-Genosys Blood card punching robot Peptide & DNA Synthesizers Petri dish plating robot Custom PAGE gel rigs

17 Copyright 2006 by BioTX Automation, Inc. BioTXs RADS Robot Tools

18 Copyright 2006 by BioTX Automation, Inc. BioTX Contacts James Frost Mary Freeman Donwick Drive, Suite A6 Conroe, TX


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