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Shear stress affects the intracellular distribution of eNOS: direct demonstration by a novel in vivo technique by Caroline Cheng, Rien van Haperen, Monique.

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Presentation on theme: "Shear stress affects the intracellular distribution of eNOS: direct demonstration by a novel in vivo technique by Caroline Cheng, Rien van Haperen, Monique."— Presentation transcript:

1 Shear stress affects the intracellular distribution of eNOS: direct demonstration by a novel in vivo technique by Caroline Cheng, Rien van Haperen, Monique de Waard, Luc C. A. van Damme, Dennie Tempel, Laurens Hanemaaijer, Gert W. A. van Cappellen, Joop Bos, Cornelis J. Slager, Dirk J. Duncker, Anton F. W. van der Steen, Rini de Crom, and Rob Krams Blood Volume 106(12): December 1, 2005 ©2005 by American Society of Hematology

2 Design of the shear stress modifier: cast model. Cheng C et al. Blood 2005;106: ©2005 by American Society of Hematology

3 Effect of cast placement around carotid arteries on eNOS-GFP fluorescence. Cheng C et al. Blood 2005;106: ©2005 by American Society of Hematology

4 Intracellular localization of eNOS-GFP and Golgi complex in areas of the carotid artery experiencing different shear stress patterns after 2 days of cast placement. Cheng C et al. Blood 2005;106: ©2005 by American Society of Hematology

5 Intracellular localization of eNOS-GFP and PECAM-1 in regions of the carotid artery experiencing different shear stress patterns after 2 days of cast placement. Cheng C et al. Blood 2005;106: ©2005 by American Society of Hematology

6 The correlation between the Golgi complex area versus eNOS-GFP area. Cheng C et al. Blood 2005;106: ©2005 by American Society of Hematology

7 Intracellular localization of eNOS-GFP and phospho-eNOS in areas of the carotid artery experiencing different shear stress patterns after 2 days of cast placement. Cheng C et al. Blood 2005;106: ©2005 by American Society of Hematology


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