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Relatieve affiniteit voor de alfa en beta receptor.

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Presentation on theme: "Relatieve affiniteit voor de alfa en beta receptor."— Presentation transcript:

1 Relatieve affiniteit voor de alfa en beta receptor

2 Adapted from Boterberg, 2000 E2E2 

3 GENISTEIN, DAIDZEIN, AND EQUOL AND CELL-CELL ADHESION IN MCF- 7/6 CELLS DMSOEquol Equol + ICI 182,780 Equol + MB2 SLOW AGGREGATION ASSAY

4 FAST AGGREGATION ASSAY

5 Het grote probleem variatie in resultaten van studies bij fyto oestrogenen is de grote variatie in opname van deze stoffen.

6 Agreement Nr. RT-05/02-FYTOES-1 Ghent, January12 th 2009 WORK PACKAGE I O-desmethylangolensinEquol 8-prenylnaringenin DaidzeinIsoxanthohumol Enterodiol (END)Enterolactone (ENL) Secoisolariciresinol  Large interindividual variation

7 Daidzein OHO O OH Dihydrodaidzein (DHD) OHO O O H Equol OHO OH O-desmethylangolensin (O-DMA) OHO O O H H SOY Isoxanthohumol (IX) 8-Prenylnaringenin (8-PN) OMe OH HOP Agreement Nr. RT-05/02-FYTOES-1 Ghent, January12 th 2009

8 MIX Daidzein OHO O OH Equol OHO OH IX OMe 8-PN OH ENL SECO R: H GUT MICROBIOTA Daidzein OHO O OH Equol OHO OH IX OMe 8-PN OH ENL SECO R: H GUT MICROBIOTA

9 Agreement Nr. RT-05/02-FYTOES-1 Ghent, January12 th 2009 WORK PACKAGE I Individual phytoestrogens – SHIME run Individual phytoestrogens – SHIME run

10 Agreement Nr. RT-05/02-FYTOES-1 Ghent, January12 th 2009 WORK PACKAGE I Individual phytoestrogens – SHIME run Individual phytoestrogens – SHIME run Daidzein metabolism (Decroos et al. (2006) J Nutr 136, ) Daidzein metabolism (Decroos et al. (2006) J Nutr 136, ) Weak equol-producing inoculum + EPC4 Weak equol-producing inoculum + EPC4 Standard SHIME feed µM daidzein aglycon eq. Standard SHIME feed µM daidzein aglycon eq. IX metabolism (Possemiers et al. (2006) J Nutr 136, ; Possemiers et al. (2008) J Nutr 138, ) IX metabolism (Possemiers et al. (2006) J Nutr 136, ; Possemiers et al. (2008) J Nutr 138, ) Strong 8-PN-producing inoculum Strong 8-PN-producing inoculum Weak 8-PN-producing inoculum + E. limosum Weak 8-PN-producing inoculum + E. limosum Standard SHIME feed + 70 µM IX Standard SHIME feed + 70 µM IX  Stable EQUOL production in colon transversum  Stable 8-PN production in colon transversum

11 BREAST TISSUE HOMOGENATE

12 MATERIAL & METHODS - BREAST FFQ, BMI, age, antibiotic use,… bloodspot urine Breath methane R WASHOUT TREATMENT GC-FID AESTHETIC BREAST SURGERY Breast biopsies Enzymatic deconjugation Extraction HPLC-UV-MS Enzymatic deconjugation Extraction HPLC-UV-MS Bio-activity biodistribution concentration molecular form


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