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Flow chart detailing the scoring system for the random spore analysis Colony from SPO plate resuspended in sterile distilled water...... CAN.... CAN +

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Presentation on theme: "Flow chart detailing the scoring system for the random spore analysis Colony from SPO plate resuspended in sterile distilled water...... CAN.... CAN +"— Presentation transcript:

1 Flow chart detailing the scoring system for the random spore analysis Colony from SPO plate resuspended in sterile distilled water CAN.... CAN + G418 CAN + G418+DOX CAN + NAT CAN + NAT+DOX CAN + G418 +NAT+DOX CAN + G418 +NAT Synthetic Sick The colonies on all the plates (G418, G418+DOX, NAT, NAT+DOX, G418+NAT) look normal but those on G418+NAT+DOX are very small. Comparing the 6 plates (G418, G418+DOX, NAT, NAT+DOX, G418+NAT, G418+NAT+DOX) at 65 hrs CAN CAN + G418 CAN + G418+DOX CAN + NAT CAN + NAT+DOX CAN + G418 +NAT+DOX CAN + G418 +NAT Synthetic lethal The colonies on all the plates (G418, G418+DOX, NAT, NAT+DOX, G418+NAT) look normal but there are no colonies on G418+NAT+DOX.... Fig 1 Suppl Big colonies Small colonies (double mutants)

2 For strains that are slow on doxycycline Colony from SPO plate resuspended in sterile distilled water CAN. CAN + G418 CAN + G418+DOX CAN + NAT CAN + NAT+DOX CAN + G418 +NAT+DOX CAN + G418 +NAT No growth on CAN+G418+DOX 65hrs. CAN CAN + G418 CAN + G418+DOX CAN + NAT CAN + NAT+DOX CAN + G418 +NAT+DOX CAN + G418 +NAT Synthetic lethal..... Check plates every 24hrs until well formed colonies seen on CAN+ G418+DOX

3 TSC11 CDC40 ts X tet0 7 -PRP24 SD-His/Arg +canavanine SD-His/Arg +canavanine +G418 SD-His/Arg +canavanine +G418+dox SD-His/Arg +canavanine +G418+nat SD-His/Arg +canavanine +nat SD-His/Arg +canavanine +G418+nat +dox SD-His/Arg +canavanine+ nat+dox SD-His/Arg +canavanine SD-His/Arg +canavanine +G418 SD-His/Arg +canavanine +G418+dox SD-His/Arg +canavanine +G418+nat SD-His/Arg +canavanine +nat SD-His/Arg +canavanine +G418+nat +dox SD-His/Arg +canavanine +nat+dox CDC40 ts X tet0 7 -PRP38

4 PRP38CKS1 CDC40 ts X tet0 7 -PRP16CDC40 ts X tet0 7 -CKS1 SD-His/Arg +canavanine SD-His/Arg +canavanine +G418 SD-His/Arg +canavanine +G418+dox SD-His/Arg +canavanine +G418+nat SD-His/Arg +canavanine +nat SD-His/Arg +canavanine +G418+nat +dox SD-His/Arg +canavanine+ nat+dox SD-His/Arg +canavanine SD-His/Arg +canavanine +G418 SD-His/Arg +canavanine +G418+dox SD-His/Arg +canavanine +G418+nat SD-His/Arg +canavanine +nat SD-His/Arg +canavanine +G418+nat +dox SD-His/Arg +canavanine +nat+dox

5 CDC40 ts X tet0 7 -RTS2CDC40 ts X tet0 7 -RFA2 SD-His/Arg +canavanine SD-His/Arg +canavanine +G418 SD-His/Arg +canavanine +G418+dox SD-His/Arg +canavanine +G418+nat SD-His/Arg +canavanine +nat SD-His/Arg +canavanine +G418+nat +dox SD-His/Arg +canavanine+ nat+dox SD-His/Arg +canavanine SD-His/Arg +canavanine +G418 SD-His/Arg +canavanine +G418+dox SD-His/Arg +canavanine +G418+nat SD-His/Arg +canavanine +nat SD-His/Arg +canavanine +G418+nat +dox SD-His/Arg +canavanine +nat+dox

6 CDC40 ts X tet0 7 -PSF2CDC40 ts X tet0 7 -TSC11 SD-His/Arg +canavanine SD-His/Arg +canavanine +G418 SD-His/Arg +canavanine +G418+dox SD-His/Arg +canavanine +G418+nat SD-His/Arg +canavanine +nat SD-His/Arg +canavanine +G418+nat +dox SD-His/Arg +canavanine+ nat+dox SD-His/Arg +canavanine SD-His/Arg +canavanine +G418 SD-His/Arg +canavanine +G418+dox SD-His/Arg +canavanine +G418+nat SD-His/Arg +canavanine +nat SD-His/Arg +canavanine +G418+nat +dox SD-His/Arg +canavanine +nat+dox

7 CDC40 ts X tet0 7 -HSP60CDC40 ts X tet0 7 -KAP95 SD-His/Arg +canavanine SD-His/Arg +canavanine +G418 SD-His/Arg +canavanine +G418+dox SD-His/Arg +canavanine +G418+nat SD-His/Arg +canavanine +nat SD-His/Arg +canavanine +G418+nat +dox SD-His/Arg +canavanine+ nat+dox SD-His/Arg +canavanine SD-His/Arg +canavanine +G418 SD-His/Arg +canavanine +G418+dox SD-His/Arg +canavanine +G418+nat SD-His/Arg +canavanine +nat SD-His/Arg +canavanine +G418+nat +dox SD-His/Arg +canavanine +nat+dox

8 CDC40 ts X tet0 7 -GPI10CDC40 ts X tet0 7 -GWT1 SD-His/Arg +canavanine SD-His/Arg +canavanine +G418 SD-His/Arg +canavanine +G418+dox SD-His/Arg +canavanine +G418+nat SD-His/Arg +canavanine +nat SD-His/Arg +canavanine +G418+nat +dox SD-His/Arg +canavanine+ nat+dox SD-His/Arg +canavanine SD-His/Arg +canavanine +G418 SD-His/Arg +canavanine +G418+dox SD-His/Arg +canavanine +G418+nat SD-His/Arg +canavanine +nat SD-His/Arg +canavanine +G418+nat +dox SD-His/Arg +canavanine +nat+dox

9 CDC40 ts X tet0 7 -YGL047WCDC40 ts X tet0 7 -FRQ1 SD-His/Arg +canavanine SD-His/Arg +canavanine +G418 SD-His/Arg +canavanine +G418+dox SD-His/Arg +canavanine +G418+nat SD-His/Arg +canavanine +nat SD-His/Arg +canavanine +G418+nat +dox SD-His/Arg +canavanine+ nat+dox SD-His/Arg +canavanine SD-His/Arg +canavanine +G418 SD-His/Arg +canavanine +G418+dox SD-His/Arg +canavanine +G418+nat SD-His/Arg +canavanine +nat SD-His/Arg +canavanine +G418+nat +dox SD-His/Arg +canavanine +nat+dox


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