3Immunospuppression in transplantation Aim of immunosuppression:Prevent acute rejection, while avoiding infection and toxicity.The primary efficacy end-points of immunosuppression:The incidence of clinical or biopsy-proven acute rejection in the first six months or one year. Graft survival and patient survival are also reported.Secondary end-points of immunosuppression:The incidence of side-effects, such as infection, renal dysfunction, diabetes, and lymphoproliferative disease.
4Calcineurin inhibitor (CNI) Cyclosporin: cyclic peptide Tacrolimus: macrolideAnti-metabolitesAzathioprineMycophenolate mofetil (MMF)GlucocorticoidsMethylprednisolonePrednisoloneTOR inhibitorsSirolimus: macrolideEverolimusATG (anti-thymocyte globulin) or interleukin-2 receptor antagonists, such as basiliximab or daclizumab
5The combinations of drugs and variations in the doses, formulations, timing, and weaning schedules are almost endless.
6Adverse Effects of Immunosuppressants 1. the toxicity resulting from over-immunosuppression (eg, infection)2. Drug-specific adverse reactions.
10Side effects of TOR inhibitors The main side effects are: hypercholesterolaemiainfectionacnemouth ulcerspneumonitisgastrointestinal side effectswound dehiscenceNephrotoxicity, neurotoxicity, hypertensionand glucose intolerance are not usually observed.
11Case Report Age: two year-old Sex: female ABO-incompatible heart transplantation at four months of age.Maintenance immunosuppression included:tacrolimus (FK506) 0.8 mg p.o. q12 hmycophenolate mofetil 100 mg p.o. q12 h.prednisolone
12-3 weeks: routine echocardiography and EMBx were normal. Day -2 to day 0: recurrent fever with no focusprogressive anemiarenal dysfunction in a local hospital.Day 1: Transfered to HSCsigns of congestive heart failure.ECG revealed ischemic changes.decreased LVEF with dilatation and severe right ventricular dysfunction. One small arterial vessel exhibited intimal hyperplasia consistent with CAD.Liver transaminases were mildly elevated (AST 69 umol/L, ALT 49 umol/L).INR was 1.64 and albumin was 24 g/L.Hemoglobin was low (72 g/ L)Renal dysfunction progressed.The patient was transfused with packed red blood cells. (Normalized hemoglobin)Methylprednisolone and Thymoglobulin® was discontinued.The patient was re-listed for another heart transplant.
13Day 2 to 6: Tacrolimus was held on day 2 for five days because of a reported trough blood concentration >30 ug/L [EMIT immunoassay, Viva Analyzer (Dade Behring, Deerfield, IL, USA)].The patient required one-fourth of her pre-admission maintenance dose (0.02 mg/kg/day orally) to keep trough concentrations within the target range of 8–10 ug/L.Potential influencing factors such as liver dysfunction, decreased enteral intake (resulting in increased tacrolimus absorption) and drug interaction (concomitant pulse methylprednisolone) resulting in decreased calcineurin inhibitor metabolism and excretion were present.Day 5: Liver transaminases had normalized. Albumin was 27 g/L.
14Day 7: Nadolol and amiodarone were prescribed on day 7 to control arrhythmia. Day 8: Tacrolimus trough concentrations reported day 8 and day 10 were within the target range.Day 10: Conjugated bilirubin was zero and the unconjugated fraction was eight.Day 11: Sirolimus was prescribed on day 11 in an attempt to augment immunosuppression yet spare calcineurin inhibitor exposure given the patient’s renal dysfunction.The initial dose of 1 mg p.o. daily (0.06 mg/kg/day) was low in relation to our centre’s experience with children in this age group.Day 13: Given that tacrolimus was to be discontinued, sirolimus was then increased to 2 mg p.o. daily (0.12 mg/kg/day).
15Day 16: Sirolimus was held. The target trough concentration was 10 ug/L. The trough concentration (Ultima Tandem Mass Spectrometer, Waters Ltd., Centennial Park, Elstree, Hertfordshire, UK) was 52.7 ug/L.Tacrolimus was discontinued. Trough concentration was 12.9 ug/LDay 17-30: Sirolimus blood concentrations remained above the target range for an additional 14 days.Day 31: Once the trough concentration normalized, sirolimus was restarted at a smaller dose 0.5 mg p.o. daily (0.03 mg/kg/day).Day 32: The dose was further decreased to 0.3 mg p.o.daily (0.02 mg/kg/day). trough concentration = 17.2 ug/L.Day 37: The trough blood concentration was measured after five days oftherapy and was reported as 13.4 lg/L, still above the target concentration.Day 42: The patient succumbed because of complications of ECMO.
16Triglycerides and cholesterol were not measured during the hospital course; however, blood samples were intermittently reported as lipemic.Amiodarone serum concentrations were not analyzed.
191. Narrow therapeutic index Therapeutic index = toxic dose/minimal effective dosegraft rejectionadverse effectsDesirable pharmacological effect only within narrow ranges of concentration in the blood.
202. Poor dose response relationship Highly variable absorption, distribution and metabolism between and within patients.Poor solubility in waterDrug-drug interactionPolymorphism or defectiveness in key enzymes responsible for metabolizing immunosuppressants .
213. The clinical effect is difficult to measure Graft rejection may be the first indication of therapeutic ineffectiveness.The major adverse effect of the drug is hard to distinguish from a lack of therapeutic effects.
22Common medications taken by transplant recipients ImmunosuppressantsAntidepressantsAntimicrobialsCholesterol-lowering AgentsAntihypertensivesAnti-ulcer Medications
24Pharmacodynamic interactions Drug-drug interactions that modulate the drug's effect in the absence of a corresponding change in plasma concentration.Examples include interactions at the level of receptor binding or secondary messenger systems.For example, an increase in renal toxicity is observed with the co- administration of naproxen and CsA.
25Pharmacokinetic interactions Drug-drug interactions that alter the plasma concentration of 1 or both drugs.Drug-metabolizing enzymesDrug transporters
26Common Enzymes Involved in Immunosuppressant Metabolism Cytochrome P450s (CYPs), ie, 3A4/5,CsA, SIR, and TACUDP-glucuronosyltransferases (UTGs)MPAXanthine oxidaseAZAFlavin mono-oxygenases, sulfotransferases, and N-acetyl transferases???
27UDP-glucuronosyltransferases (UTGs) in MPA Metabolism The UGT superfamily is composed of 2 families: UGT1 and UGT2.Conjugate substrates with the addition of a glucuronyl group.The rate-limiting step in the metabolism of MPA (active form of MMF).
28Xanthine oxidase in AZA Metabolism AZA 6-mercaptopurine inactivation by xanthine oxidaseallopurinol inhibits xanthine oxidase, co-administration of allopurinol and azathioprine can result in increased levels of 6- mercaptopurine.
29CYPsThe most prominent family of drug-metabolizing enzymes.
31Drug transporters and Immunosuppressant Major transporterP-glycoprotein (P-gp), the protein product of the multidrug resistance 1 gene (MDR1).CsA and TAC (substrates, inhibtors, inducers).SIR (inhibtor, inducer)Other transportersmultidrug resistance gene 2/3,organic anion transporter proteinsmultidrug resistance-related proteins (MRP1-6)Sister of P-glycoprotein (S-Pgp)
34CYP3A and P-gp Substrates (S), Inhibitors (I), Inducers(In), and Activators (A) CYP3A P-gpImmunosuppressants Cyclosporine (S, I) Cyclosporine (S, I, In) Methylprednisolone (I) Methylprednisolone (S) Sirolimus (S, I) Prednisolone (S, In) Tacrolimus (S) Sirolimus (I, In) Tacrolimus (S, I, In)Antibiotics/antifungal agents Clarithromycin (S) Clarithromycin (I) Clotrimazole (I) Clotrimazole (In) Erythromycin (S, I) Erythromycin (S, I, In) Itraconazole (S, I) Itraconazole (S, I) Ketoconazole (S, I) Ketoconazole (I) Sparfloxacin (S)Antihistamines Astemizole (S, I) Astemizole (I) Cimetidine (I) Chlorpromazine (I) Loratidine (S) Cimetidine (S) Terfenadine (PS, I) Fexofenadine (S) Ranitidine (S)
35CYP3A P-gpPsychoactive agents/anticonvulsants Amitriptyline (S) Amitriptyline (S) Carbamazepine (In) Midazolam (S, I) Midazolam (I, In) Nefazodone (S, I) Nefazodone (I) Phenobarbital (In) Nortriptyline (S) Phenobarbital (In) Phenytoin (S, In) Hormonal agents Cortisol (S) Aldosterone (S) Dexamethasone (S, I) Cortisol (I) Estradiol (S, I) Dexamethasone (S, In) Hydrocortisone (S) Estradiol (In, MS) Progesterone (S, I) Hydrocortisone (S, I) Testosterone (S, A) Insulin (In) Progesterone (I) Testosterone (I)Other cytotoxics Colchicine (S) Colchicine (S, In) Mitoxantrone (S, In)
36CYP3A P-gpCardiac agents Amiodarone (S) Amiodarone (I, In) Atorvastatin (S) Atorvastatin (I) Digitoxin (PS) Celiprolol (S) Diltiazem (S, I) Digoxin (S) Felodipine (S) Diltiazem (S, I, In) Fluvastatin (S) Felodipine (I) Lidocaine (S) Lidocaine (I) Lovastatin (S) Nicardipine (I, In) Nicardipine (S, I) Nifedipine (In) Nifedipine (S, I) Nitrendipine (I) Nitrendipine (S, I) Quinidine (S, I) Pravastatin (S) Talinolol (S) Quinidine (S, I) Terfenadine (I) Simvastatin (S) Verapamil (S, I, In) Verapamil (S, I) HIV agents Amprenavir (S, I) Amprenavir (S, In) Indinavir (S, I) Indinavir (S) Nelfinavir (S, I) Nelfinavir (S, I, In) Ritonavir (S, I) Ritonavir (S, I, In) Saquinavir (S, I) Saquinavir (S, I)
37CYP3A P-gpAnticancer drugs Busulfan (S) Actinomycin D (S) Docetaxel (S) Cisplatin (S, In) Doxorubicin (S) Cytarabine (S) Etoposide (S) Daunorubicin (S, In) Paclitaxel (S) Docetaxel (S) Tamoxifen (S) Doxorubicin (S, In) Vinblastine (S, I) Etoposide (S, In) Vincristine (S, I) Fluorouracil (S, In) Vindesine (S) Methotrexate (S, In) Mitomycin (S) Paclitaxel (S) Taxol (S) Tamoxifen (I, In) Vinblastine (S, I, In) Vincristine (S, In) Vindesine (S)Benzodiazepines Alprazolam (A) Clonazepam (S) Midazolam (S)Triazolam (S)
38CYP3A P-gpOpiates Methadone (S, I) Fentanyl (S) Loperamide (S) Methadone (S, I) Morphine (S, In)Others Grapefruit juice (I) Bepridil (I) Losartan (S) Bromocriptine (In) St. John's wort (In) Chlorambucil (S, In) Dipyridamole (I) Disulfiram (I) Grapefruit juice (A) Hydroxyurea (S, In) Losartan (S) Octreotide (S) Phenothiazine (I) Probenecid (In) Quinine (I) Reserpine (I, In) Rifampin (In) St. John's wort (In) Tetrabenazine (I) Topotecan (S) Valinomycin (I) Yohimbine (In)
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