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Multienzyme systems A soluble or dissociated multienzyme system with diffusing intermediates A, B, C E1E1 E2E2 E3E3 E4E4 SABCP.

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Presentation on theme: "Multienzyme systems A soluble or dissociated multienzyme system with diffusing intermediates A, B, C E1E1 E2E2 E3E3 E4E4 SABCP."— Presentation transcript:

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2 Multienzyme systems A soluble or dissociated multienzyme system with diffusing intermediates A, B, C E1E1 E2E2 E3E3 E4E4 SABCP

3 Multienzyme systems Multienzyme complex Intermediates in the reaction sequence catalyzed by an enzyme complex are covalently bound and do no diffuse away from the complex E1E1 E2E2 E3E3 E4E4 E5E5 E6E6 E7E7

4 Multienzyme systems Membrane bound enzyme systems Large supramolar structures such as membranes or ribosomes Terminal oxydation chain

5 I. II. III. Cit c IV. NADH + H + Szukcinát Fumarát 4 H + 2 H UQ e- e- e- e- e-e-e-e- 4 H + + O 2 H2OH2OH2OH2O H+H+H+H+ H+H+H+H+ H+H+H+H+ H+H+H+H+ H+H+H+H+ NAD +

6 Kinetical behaviour Each component has an own K m for substrate, cofactor, V max and pH optimum Computer modell Regulatory enzyme Fatty acid synthetase Pyruvate- dehydrogenase.  -ketoglutarate dehydrogenase Branched chain amino acid dehydrogenase Multienzyme systems

7 Pyruvate-dehydrogenase (PDH) Glucose Pyruvate Pyruvate-dehydrogenase enzyme complex glycolysis

8 Piruvát - dehidrogenáz Kofaktorok: NAD + HS-CoAKoenzimek Tiamin-pirofoszfát (TPP) Liponsav FAD Prosztetikus csoportok

9 A piruvát-dehidrogenáz multienzim komplex (PDH – komplex) E 1 : Piruvát dehidrogenáz  2  2 (20-30) E 2 : Dihidrolipoil-transzacetiláz (60) x: alegység (6) E 3 : Dihidrolipoil-dehidrogenáz (6) Protein-kináz Foszfoprotein-foszfatáz E1E1 E1E1 TPP- X E3E3 E2E2 FAD

10 A PDH komplex szabályozása E1E1 E1E1 X E3E3 E2E2 E1E1 E1E1 X E3E3 E2E2 PDH-kináz ATP ADP PDH-foszfatáz H2OH2O HPO ATP NADH + H + Acetil-CoA - Piruvát (máj) + Inzulin (zsírszövet) Ca 2+ ADP, NAD+ HS-CoA koncentráció növekedése esetén aktív inaktív


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