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Morphological structure and water status in bulbs during their transition from dormancy to active growth: Visualization by NMR imaging Rina Kamenetsky.

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Presentation on theme: "Morphological structure and water status in bulbs during their transition from dormancy to active growth: Visualization by NMR imaging Rina Kamenetsky."— Presentation transcript:

1 Morphological structure and water status in bulbs during their transition from dormancy to active growth: Visualization by NMR imaging Rina Kamenetsky Hanita Zemah Annette van der Toorn Haim D. Rabinowitch Henk van As Peter Bendel accepted by J. exp. Bot.

2 Tulip bulbs (Tulipa gesneriana L.) World wide use of tulip as ornamental plant Needs a cold period during dormancy to facilitate normal development and flowering Fundamental knowledge about bulb dormancy and growth is limited

3 Purpose of the project: To determine if significant differences in water status between bulbs stored at 4 o C and at 20 o C can be observed using MRI. Mainly using: Visible morphological changes in the images Proton density determination R 2 measurements (1/T 2 ) ADC measurements (reflecting proton mobility) R 1 measurements (1/T 1 )

4 scales bud basal plate 1 cm Morphological MR image of tulip bulb Resolution 350 x 350 x 700  m

5 e Single parameter images of the tulip bulb Amp of R1Amp of R2Amp of ADC R1 imageR2 imageADC image

6 o C4 o C 20 o C amplitude R2 start 2 weeks 4 weeks 8 weeks 12weeks Time- dependent change in amplitude for two bulbs Time- dependent change in R 2 for two bulbs

7 Planted bulbs (10 days after planting) 4 o C20 o C

8 Conclusions MR parameters such as R 2 and ADC can be used to observe differences between chilled and non-chilled bulbs MRI needs to be combined with histology to understand the observed changes in water status The majority of changes in water status take place between 2 and 8 weeks after storage


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