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PEDRIGREE AND GENETIC ANALISYS OF THE ISTRIAN CATTLE Ivanković A. 1, Ramljak J. 1, Šubara G. 2, Kelava N. 1, Prekalj G. 2 (1) Department of Animal Production.

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Presentation on theme: "PEDRIGREE AND GENETIC ANALISYS OF THE ISTRIAN CATTLE Ivanković A. 1, Ramljak J. 1, Šubara G. 2, Kelava N. 1, Prekalj G. 2 (1) Department of Animal Production."— Presentation transcript:

1 PEDRIGREE AND GENETIC ANALISYS OF THE ISTRIAN CATTLE Ivanković A. 1, Ramljak J. 1, Šubara G. 2, Kelava N. 1, Prekalj G. 2 (1) Department of Animal Production and Technology, Agricultural Faculty, University of Zagreb, Svetošimunska 25, Zagreb, Croatia. (2) AZRRI – Agency for rural development of Istria Ltd. Pazin; Agenzia per lo sviluppo rurale dellIstria Pisino

2 Introduction autochthonous cattle breeds in Croatia number of animals drastically declined systematic upgrading – 138 animals; 2008 – 460 animals institutional measures (subsidies, promotions), established association (AZRRI), economic reaffirmations (brand beef production) Sulle tracce delle Podoliche – On the Tracks of Grey Podolic cattle

3 Aim –analyze of pedigree and genetic variability within Istrian Cattle shaping breeding programmes and preserving strategies Sulle tracce delle Podoliche – On the Tracks of Grey Podolic cattle

4 Geographical location of Istrian Cattle populations Material & Methods Pedigree information data (1994 – 2008.) ENDOG v4.5 program Sulle tracce delle Podoliche – On the Tracks of Grey Podolic cattle Blood samples 51 unrelated animals Fstat v2.9.3, GenePop 3.4., Bottleneck v program 30 microsatellites belong to the panel of the MoDAD marker sets recommended by FAO

5 Generation intervalNumber of herds Pathway Mean SE ActualEffective Father – son HSHS Father – daughter H SS Mother – son H SSS Mother – daughter H SSSS Total Results Sulle tracce delle Podoliche – On the Tracks of Grey Podolic cattle Generation interval and number of herds contributing sires (H S ), grand-sires (H SS ), great-grandsires (H SSS ) in Istrian cattle.

6 Number of alleles (MNA), observed and expected heterozygosity (H o, H E ), polymorphism information content (PIC), fixation coefficient (F IS ) at 30 microsatellites. Mean 0.071*** Media TGLA TGLA TGLA TGLA NCAM MM INRA ** INRA INRA INRA ** INRA INRA ILSTS ILSTS HEL9 Marcatore Locus MNAHOHO HEHE PICF IS BM BM BM ** CSRM CSSM ETH ETH ETH ETH * ETH HAUT HAUT HEL * HEL HEL Marcatore Locus MNAHOHO HEHE PICF IS

7 Estimates of average inbreeding and the average relatedness in Istrian cattle Complete equivalent generations Average F (%) in the whole pedigree Average relatedness (AR) (%) Inbred animals (%) Average F (%) of inbred animals low pedigree completeness mating between relatives high degree of relationships and migration weaker pedigree completeness, more precision mating scheme Sulle tracce delle Podoliche – On the Tracks of Grey Podolic cattle

8 absence of bottleneck using Wilcoxon test (TPM)

9 Conclusions Sulle tracce delle Podoliche – On the Tracks of Grey Podolic cattle Istrian cattle did preserve part of genetic diversity presence of inbreeding, with absence of bottleneck unsystematic pedigree data DNA testing still endangered breed – continuous monitoring of population parameters, – adjusting breeding program preserving Istrian cattle breeds have to have high national and international priority

10 We thank all breeders and Reproduction Center for providing samples as well as Croatian Agriculture Agency. The study of Croatian breeds was partly supported by the Ministry of Science, Education and Sports, Croatia. Acknowledgements:


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